MitoLipin - Liquid Saturated Phosphatidylcholine (PC) Mix

[Andman]

So i spilled about 1/4 of a bottle on my rats arm by accident. Other than being very thirsty today the only side effect ive noticed is a great mood (very social, talkative etc.)
@haidut anything i should be worried about?

 

[Momado965]

After about a week of taking 200mg, I felt like I was getting uncoupling effects and lost some weight. My waking temps were around 99 but pulse did not change. It also made me able to focus and do much longer stretches of mental effort. Mood improved a lot as well. Similar to the effects of a very high dose methylene blue (60mg). However, with that dose of methylene blue I experienced very pronounced increase in serotonin and it is not something that can be maintained for more than a few days. With MitoLipin (both topical and oral) I felt much more blissful, without the nervousness from the serotonin of high dose methylene blue. I guess the effects I got from MitoLipin are not that surprising since it acts very similar to methylene blue but through a different pathway. Methylene blue is an electron carrier and alternative oxidizing agent. MitoLipin acts like an antagonist of PUFA, so re-saturates the mitochondrial lipids. So, its effects are a bit like depleting yourself of PUFA but you don't have to wait 4 years to accomplish that
I hope that answers your questions.

So you took 40 drops or 80 drops for two weeks?

 

[shine]

Amazing product. Took my first dose today and immediately noticed benefits.
At first I got really calm and had a sensation of pure bliss, I just sat on my bed nodding like a druggie.
Then I noticed twitching muscles in my back and over the course of about 15 minutes my whole back relaxed bit by bit and all the pain and discomfort I had there for months/years vanished. It is unbelievable to me.
My mood is incredibly good now and I feel wide awake, but at the same time I am incredibly relaxed and not jittery.
Didn't expect such a drastic effect, atleast not that quickly.
Thank you @haidut !

 

[Hans]

Amazing product. Took my first dose today and immediately noticed benefits.
At first I got really calm and had a sensation of pure bliss, I just sat on my bed nodding like a druggie.
Then I noticed twitching muscles in my back and over the course of about 15 minutes my whole back relaxed bit by bit and all the pain and discomfort I had there for months/years vanished. It is unbelievable to me.
My mood is incredibly good now and I feel wide awake, but at the same time I am incredibly relaxed and not jittery.
Didn't expect such a drastic effect, atleast not that quickly.
Thank you @haidut !

Sweet. Did you apply it topically or take it orally?

 

[haidut]

So i spilled about 1/4 of a bottle on my rats arm by accident. Other than being very thirsty today the only side effect ive noticed is a great mood (very social, talkative etc.)
@haidut anything i should be worried about?

Not really, nothing in the product is toxic especially when simply spilled on the skin.

 

[haidut]

Amazing product. Took my first dose today and immediately noticed benefits.
At first I got really calm and had a sensation of pure bliss, I just sat on my bed nodding like a druggie.
Then I noticed twitching muscles in my back and over the course of about 15 minutes my whole back relaxed bit by bit and all the pain and discomfort I had there for months/years vanished. It is unbelievable to me.
My mood is incredibly good now and I feel wide awake, but at the same time I am incredibly relaxed and not jittery.
Didn't expect such a drastic effect, atleast not that quickly.
Thank you @haidut !

Awesome, thanks for the great feedback!

 

[shine]

Sweet. Did you apply it topically or take it orally?

Topically, wrists and lower belly area.

 

[Broken man]

Is there chance that phosphatidilcholine can dissolve lipofuscin?

 

[Fractality]

No mixing with water, the PC is so hydrophobic that it will immedietaly precipitate and form a whitish cloud inside the liquid. For the people that choose on their own volition to take orally, taken on its own is best. It has enough fat and vitamin E in it to ensure absorption.

I've never noticed this. It turns into a globule that sits on top of the water surface.

 

[Hazarlar]

8 drops and The Mammal literally "trips". ... Can it be stored in a fridge as my room teperatures may hit +25C in summer?

 

[haidut]

8 drops and The Mammal literally "trips". ... Can it be stored in a fridge as my room teperatures may hit +25C in summer?

No fridge, the saturated PC will make the liquid literally solidify. It is vitamin E and saturated fat basically, so it can least years at room temp but FDA mandates no more than 12 months on all liquid supplements.

 

[haidut]

Is there chance that phosphatidilcholine can dissolve lipofuscin?

Possibly yes, even though there is more research on vitamin E for that purpose (which MitoLipin also contains). Google for "vitamin E lipofuscin" (no quotes) for more info.

 

[LucyL]

Mitogenic effects of phosphatidylcholine nanoparticles on MCF-7 breast cancer cells.
Results suggest that nanoparticles derived from PC dispersion prepared in buffer pH 7.0 may induce physicochemical changes in the plasma membrane of cancer cells which may affect EGFR cellular localization and/or activity, increasing activation of the MEK-ERK1/2 pathway and inducing proliferation.

Moreover, increased levels of phosphocholine and choline-containing compounds have been associated with progression and bad prognosis of tumors. [note this statement had no references]

Results showed that PC nanoparticles prepared in neutral buffer induced the activation of the MEK-ERK1/2 pathway and increased cell viability and proliferation of the MCF-7 breast cancer cell line.

There is this though:

Increased concentration of phosphatidylcholine nanoparticles dispersed in buffer pH 7.0 had significant effects over cell proliferation, EGFR levels, and activation of the MEK1/2-ERK1/2 pathways; however, such effects were not observed for PC nanoparticles dispersed in pH 5.0 buffer. The main differences between both PC preparations is the charge associated with the particles (zeta potential) (Table 1) and the final adopted form [7], while the size of the particles did not show important differences (Table 1). The nanoparticles were in the range of 180–250 nm for all the studied conditions (Table 1). At pH 5.0, small, isolated particles with irregular shape were observed while at pH 7.0 more elongated, locally cylindrical structures were described [7]. As expected, the zeta potential of the particles was positive when using pH 5.0 buffer as diluent and negative when using pH 7.0 buffer (Table 1). This fact can be related to changes in the proportion of the differently charged forms of the zwitterionic phosphocholine polar head within the selected pH range and the conformational organization the molecules acquire as a result.

Zwitterionic?

Considering that the PC nanoparticles preformed in a pH 5.0 buffer showed no significant biological effects over the breast cancer cells, these would be safer than those prepared in a pH 7.0 buffer to deliver antimitotic agents.

So the negative results were linked to the pH of the buffer solution, it would be nice to know how the buffer solutions affected cellular mitigation if the researchers had used a saturated fat based phosphatidycholine, instead of soybean. Or maybe the phosphate buffer solution (pH 7.0) versus an acetate buffer solution (pH 5.0) - why not just use a phosphate solution at two different acidities, or acetate... did they really learn anything about PC at all?

Or perhaps the goal was to make me leery of Mitolipin just because I have BC ?

 

[Amazoniac]

Topical Administration:

• The skin-permeation-enhancing effect of phosphatidylcholine: caffeine as a model active ingredient.
• The hypocholesterolemic and antiatherogenic effects of topically applied phosphatidylcholine in rabbits with heritable hypercholesterolemia.
• Effects of phosphatidylcholine on the topical bioavailability of corticosteroids assessed by the human skin blanching assay.

General/Miscellaneous:

• Cell membranes and apoptosis: role of cardiolipin, phosphatidylcholine, and anticancer lipid analogues.
• Biochemical effects of phosphatidylcholine treatment in rats.
• Rapid stopping of A23187 action by phosphatidylcholine.
• Blockade of alpha-adrenergic receptors by analogues of phosphatidylcholine.
• The effect of mixed phosphatidylcholine liposomes on beef heart cytochrome c oxidase [proceedings].
• Cytochrome c Interaction with Cardiolipin/Phosphatidylcholine Model Membranes: Effect of Cardiolipin Protonation
• Inhibition of mammalian cytotoxic cells by phosphatidylcholine and its analogue
• Studies on cytochrome oxidase. Interactions of the cytochrome oxidase protein with phospholipids and cytochrome c.

Inflammation:

• Anti-inflammatory action of a phosphatidylcholine, phosphatidylethanolamine and N-acylphosphatidylethanolamine-enriched diet in carrageenan-induced...
• Anti-inflammatory effects of phosphatidylcholine.
• Protective effect of phosphatidylcholine on lipopolysaccharide-induced acute inflammation in multiple organ injury.
• Soy phosphatidylcholine inhibited TLR4-mediated MCP-1 expression in vascular cells.

Brain/Memory/Mood/Cognition/Nervous system:

• Protective effects of phosphatidylcholine on oxaliplatin-induced neuropathy in rats.
• Dietary phosphatidylcholine improves maze-learning performance in adult mice.
• Impairment of brain mitochondrial functions by β-hemolytic Group B Streptococcus. Effect of cardiolipin and phosphatidylcholine.
• Protective effects of a phosphatidylcholine-enriched diet in lipopolysaccharide-induced experimental neuroinflammation in the rat.
• Effect of phosphatidylcholine on explicit memory.
• A report on phosphatidylcholine therapy in a Down syndrome child.
• A REPORT ON PHOSPHATIDYLCHOLINE THERAPY IN A DOWN SYNDROME CHILD
• EARLY RESULTS FROM DOUBLE BLIND, PLACEBO CONTROLLED TRIAL OF HIGH DOSE PHOSPHATIDYLCHOLINE IN ALZHEIMER'S DISEASE
• Combination of phosphatidylcholine and pentoxifylline in the therapy of progressive obstructive stroke.

Liver/Gallbladder:

• Prevention of hepatocarcinogenesis with phosphatidylcholine and menaquinone-4: in vitro and in vivo experiments.
• Accumulation of lipid in rat liver was induced by vitamin B6 deficiency and was ameliorated by supplemental phosphatidylcholine in the diet.
• Hepatoprotective effect of phosphatidylcholine against carbon tetrachloride liver damage in mice.
• Hyperreactivity of Blood Leukocytes in Patients with NAFLD to Ex Vivo Lipopolysaccharide Treatment Is Modulated by Metformin and Phosphatidylcholin...
• Effects of phosphatidylcholine pretreatment during acute experimental biliary reflux.
• Dietary phosphatidylcholine alleviates fatty liver induced by orotic acid.
• Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis.
• The effect of phosphatidylcholine on repair processes in liver cells in acute CCl4 damage.
• Protective effect of phosphatidylcholine on restoration of ethanol-injured hepatocytes related with caveolin-1.
• Phosphatidylcholine protects against steatosis in mice but not non-alcoholic steatohepatitis.
• Hydrogenated phosphatidylcholine supplementation reduces hepatic lipid levels in mice fed a high-fat diet.
• Prevention of alcohol-induced hepatic fibrosis by phosphatidylcholine.
• Phosphatidylcholine protects against fibrosis and cirrhosis in the baboon.

Gut/Bowel/GI/Endotoxin:

• Exogenous phosphatidylcholine supplementation improves intestinal barrier defense against Clostridium difficile toxin.
• In vitro evidence that phosphatidylcholine protects against indomethacin/bile acid-induced injury to cells.
• Oral phosphatidylcholine preserves the gastrointestinal mucosal barrier during LPS-induced inflammation.
• Phosphatidylcholine for steroid-refractory chronic ulcerative colitis: a randomized trial.
• First multicenter study of modified release phosphatidylcholine "LT-02" in ulcerative colitis: a randomized, placebo-controlled trial in mesalazine...
• Oral phosphatidylcholine pretreatment decreases ischemia-reperfusion-induced methane generation and the inflammatory response in the small intestine.
• Systemic phosphatidylcholine pretreatment protects canine esophageal mucosa during acute experimental biliary reflux.
• Retarded release phosphatidylcholine benefits patients with chronic active ulcerative colitis.
• Microbial species involved in production of 1,2-sn-diacylglycerol and effects of phosphatidylcholine on human fecal microbiota.
• Protective effect of oral phosphatidylcholine on radiation-induced release of intestinal peptidases in rats.
• Phosphatidylcholine induces an increase in the production of interleukin-6 and improves survival of rats with neonatal sepsis caused by Klebsiella...
• Mucosal protection by phosphatidylcholine.
• Dietary phosphatidylcholine supplementation attenuates inflammatory mucosal damage in a rat model of experimental colitis.
• Aspirin's ability to induce intestinal injury in rats is dependent on bile and can be reversed if pre-associated with phosphatidylcholine.
• Delayed release phosphatidylcholine as new therapeutic drug for ulcerative colitis--a review of three clinical trials.
• Low-dose aspirin-induced ulceration is attenuated by aspirin-phosphatidylcholine: a randomized clinical trial.
• Phosphatidylcholine (lecithin) and the mucus layer: Evidence of therapeutic efficacy in ulcerative colitis?
• Phosphatidylcholine as a constituent in the colonic mucosal barrier--physiological and clinical relevance.
• Association of phosphatidylcholine and NSAIDs as a novel strategy to reduce gastrointestinal toxicity.
• Delayed release phosphatidylcholine in chronic-active ulcerative colitis: a randomized, double-blinded, dose finding study.
• TNF-alpha-induced up-regulation of pro-inflammatory cytokines is reduced by phosphatidylcholine in intestinal epithelial cells.
• Phosphatidylcholine reverses ethanol-induced increase in transepithelial endotoxin permeability and abolishes transepithelial leukocyte activation.
• Gastroprotective capability of exogenous phosphatidylcholine in experimentally induced chronic gastric ulcers in rats.
• Phosphatidylcholine and intraperitoneal adhesions.
• Effect of phosphatidylcholine on postoperative adhesions after small bowel anastomosis in the rat.
• Effects of phosphatidylcholine and phosphatidylinositol on acetic-acid-induced colitis in the rat.

Heart/Kidney:

• Protective role of phosphatidylcholine against cisplatin-induced renal toxicity and oxidative stress in rats.
• Intraperitoneal administration of phosphatidylcholine improves ultrafiltration in continuous ambulatory peritoneal dialysis patients.

Cancer:

• Prevention of hepatocarcinogenesis with phosphatidylcholine and menaquinone-4: in vitro and in vivo experiments.
• Mitogenic effects of phosphatidylcholine nanoparticles on MCF-7 breast cancer cells.
• Phosphatidylcholine induces growth inhibition of hepatic cancer by apoptosis via death ligands.

Obesity/Diabetes/Metabolism:

• Beneficial effects of phosphatidylcholine on high-fat diet-induced obesity, hyperlipidemia and fatty liver in mice.
• Effect of oxidized phosphatidylcholine on biomarkers of oxidative stress in rats.
• Phosphatidylcholine induces apoptosis of 3T3-L1 adipocytes.
• A nuclear-receptor-dependent phosphatidylcholine pathway with antidiabetic effects.

Bones:

• Anti-inflammatory effects of phosphatidylcholine in neutrophil leukocyte-dependent acute arthritis in rats.
• Oral phosphatidylcholine pretreatment alleviates the signs of experimental rheumatoid arthritis.

Anti-viral/anti-bacterial:

• Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.
• Reduction of HIV-1 antigen production by phosphatidylcholine containing formulations via growth inhibition of HIV-1-infected cells.
• Growth inhibition of HIV-1-infected cells and membrane alterations induced by phosphatidylcholine.
• Effect of phosphatidylcholine cholesterol liposomes on the growth of bacterial cultures.

@haidut, I suggest you to go through these again because the majority here has no relevance for what you're proposing. The purported function of phosphatidylcholine here is to be a mere carrier for its saturated fatty acids, yet if you inspect one by one, not only large amounts were often used but also polyunsaturated fats instead of saturated, how to reconcile? It's either the fatty acids or the choline compound being protective.

You're listing for example an experiment with 600 mg/kg of soybean PC injected in rats, which can be the equivalent to 1 g of choline in a human. There are experiments using 800 mg of choline a day, while Mitolipin provides about 28 mg/serving. The person would need to almost empty a entire bottle at once to match the amount. Pick 3 random ones and you'll confirm this.

 

[Momado965]

Can MCT oil be used alone without mb or thyroid? Say at a dose of 10-20 grams atop a low fat diet (very low pufa high sat fat total) used instead of mitolipin to saturate the mitochondria?

 

[Deleted member 5487]

Isn't Phosphatidylcholine what makes up the fat gobuals in unhomogenized cream?

 

[Gone Peating]

Given Ray's estimate that it should take the average person 4 years to deplete their pufa stores by eating minimal pufa, how would this assist?

From the studies you have seen is there a number estimate you would put on PC being able to speed up that process?

 

[accelerator]

I have suffered from pale stools and liver congestion for years. I had thrush and low serum cholesterol. After a few days of 40 drops/day MitoLipin and then continuing with 10 drops/day (topical), my stools are now a normal dark brown. The thrush is gone. My stools are less sticky and require less wiping, as well.

I've underestimated this product for a long time.

How long overall was it before you noticed results? still taking it?

 

[Pointless]

How long overall was it before you noticed results? still taking it?

I still take it. I wasn't expecting that effect at first, though, so I couldn't tell you when it started exactly.

 

[Momado965]

Anyone knows anything about dipalmitoylphosphatidylserine - the saturated form of phosphatidylserine.