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Archive info for old Gonadin version

haidut

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This thread is just a placeholder for the information about the ingredients in the previous version of Gonadin that we had. The new version replaced 2 of the ingredients - phytol and squalene - with flavanone and alpha-naphthoflavone. The methylated fatty acids are still there. The text below is just for information purposes since it does contain some good excerpts about vitamin K, gonadal function and the role of StAR in steroidogenesis.
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As I mentioned in a few threads before, I have always been interested in the so-called "reproductive aging", both in males and females. There is solid evidence that gonadal function declines with age and as such the synthesis and circulating levels of protective steroids like testosterone (T), progesterone (P4), DHEA, and pregnenolone (P5) declines in both men and women. Initially, this decline is buffered to a degree by increased adrenal activity and DHEA synthesis. But with advancing age, the adrenal layers that synthesis DHEA tend to atrophy, leaving cortisol and estrogen to rule unopposed.
Actually, total T levels in men do not decline with age but this appears to be due to decreased clearance, while de-novo synthesis in the Leydig cells does decline quite markedly with aging, in parallel to falling levels of pregnenolone, progesterone and DHEA. In women, after menopause progesterone, pregnenolone and DHEA levels decline just as sharply as they do in males.
Interestingly, this decline in endogenous synthesis does not seem to be due to some kind of damage or atrophy of the gonads. To the contrary, cell extracted from "old" human gonads perform just as well as cells from "young" gonads when placed in optimal laboratory conditions with sufficient amount of precursors and enzyme co-factors. This shows that the decline in gonadal function is...well...functional and not structural. So, I have been searching for substances that can help restore such optimal endogenous environment to gonads. Thyroid hormone and high NAD/NADH ratio definitely seem to play a role, but there are additional pathways that appear to be involved and administering thyroid hormone is not always optimal or even desirable. One of the more interesting studies I stumbled upon (which has been posted on the forum) demonstrated that administration of vitamin K2 (MK-4) to old rats doubled their testosterone levels without any addition of other stimulating agents like thyroid hormone, or NAD, or even precursors like pregnenolone. More importantly, Vitamin K2 did not affect pituitary hormones like LH, which are typically involved in steroidogenesis. Furthermore, vitamin K1 did not have a testosterone boosting effect so this is yet another reason for chosing MK-4 over K1 (as this question keeps coming up on the forum).
Menaquinone-4 enhances testosterone production in rats and testis-derived tumor cells

That vitamin K2 study apparently got the authors thinking what could be the reason behind this effect of boosting testosterone synthesis. Given that the quinone structure is the same in both K1 and K2, the only difference is the side chain. Vitamin K2 contains geranylgeraniol (GGOH) as a side chain, while vitamin K1 does not. So, the same authors decided to do another study using just GGOH as well as a few other related substances and examine their effects on steroidogenesis.
http://www.tandfonline.com/doi/abs/10.1080/09168451.2015.1123612?src=recsys&journalCode=tbbb20

As expected, GGOH did boost testosterone levels and in a concentration about the same as the one used in the first study with MK-4 - i.e. 10μM/L - 30μM/L. It also increased the synthesis of progesterone, which is an important direct precursor to steroid synthesis in the gonads, including synthesis of T. So, the testosterone-boosting effects of vitamin K2 (MK-4) appear to be due mostly to that side chain and not the quinone structure, which matches the results from the first study. Moreover, another similar terpene known as Phytol (POH) was even more effective than GGOH in boosting synthesis of both testosterone and progesterone in Leydig cells when used in concentrations of 30μM/L (see attached screenshots).

Furthermore, the same authors published a new study in which they not only reiterated their belief that phytol and GGOH would raise T levels but also did an in vivo study to confirm their results.
A novel function of geranylgeraniol in regulating testosterone production. - PubMed - NCBI
"...Testosterone and progesterone levels in I-10 cell culture mediums markedly increased in the presence of phytol and GGPP, but not in the presence of GOH. Meanwhile, FOH enhanced progesterone, but not testosterone levels. These results indicated that phytol and GGOH have similar effects on testosterone and progesterone production although, unexpectedly, GOH did not affect steroid production in I-10 cells. Recent researches have revealed the biological activity of compounds derived from the isoprenoid/cholesterol synthesis pathway. These isoprenoids, including FOH, GOH, and phytol, regulate various biological processes [47–49]. The results indicated that not only GGOH, but other isoprenoid derivatives, can enhance testosterone and progesterone levels, although the mechanisms by which they exert these effects are yet to be clarified."

"...We also found that dietary supplementation of MK-4 enhanced plasma testosterone levels in rats, without any alternation of plasma LH levels [45]. Based on the results of our cell-based experiments [32], we conducted further experiments on the effects of GGOH on testosterone production in animals. Eight-week-old Wistar male rats were purchased from SLC Japan (Shizuoka, Japan) and fed either GGOH supplemented (48.3 mg/kg of diet) or control diet for 10 days. Growth performance did not differ between both diets, but plasma testosterone levels in GGOH supplemented group were found to be elevated compared to that of control group, indicating that dietary supplementation of GGOH significantly elevates plasma testosterone levels (unpublished data, Figure 4). These findings provide novel mechanistic insights into the process of testosterone production and may be useful for the development of therapeutic strategies to counter age-associated declines in testosterone levels in men. In summary, the novel role of GGOH in steroidogenesis may bring new possibilities and could be useful in the development of therapeutics for the treatment of men with LOH."

Since the in vitro tests in the previous studies used the same concentration of phytol and GGOH (100 uM/L), we can use the new in vivo study to estimate the HED dose for phytol. The in vivo study used GGOH in a dose of 48.3 mg/kg of diet in rats, which is 4.83 mg/kg of bodyweight, which is an HED of 0.8 mg/kg. Since phytol has slightly higher molar mass than GGOH the HED for phytol would be 0.816 mg/kg and thus the 100mg a dose of Gonadin provides should be more than enough to replicate this study. As you can see from the attached image, T levels doubled and did so in just 10 days of supplementing!

Phytol - Wikipedia
Phytol is quite an interesting substances as it is a precursor to both vitamin E and K and is used as raw material by some organisms who can synthesize these vitamins endogenously. More importantly, both of these vitamins have been found to raise T levels in animal (and some human) studies, which seems to be due to the presence of that side chain (derived from phytol) attached to the quinone ring. In addition, it was also discovered that phytol is a potent aromatase inhibitor with an IC50 of just 1μM/L, which is quite easily achievable with the amounts present is our product. In fact, in this study phytol (code-named SA-20 in the study and attached screenshots) was as effective as formestane (abbreviated FOR in the study and attached screenshots) in inhibiting estradiol synthesis when used in concentration of 1μM/L (see attached image). Formestane is a steroidal aromatase inhibitor similar to but more potent than exemestane. Perhaps even more importantly, phytol reduced mRNA expression of aromatase itself for up to 24 hours, which suggests that it acts as a long lasting "suicide aromatase inhibitor" just like formestane. In concentrations of 10μM/L, phytol was again as effective as formestane in decreasing actual aromatase expression. As mentioned above, the 100mg dose of phytol per serving (8 drops) of Gonadin should achieve 30μM/L concentrations, so the methods of the study on aromatase should be quite easily replicated. Overall, the aromatase inhibition effects of phytol could very well be responsible at least partly for the observed increase in testosterone levels, in addition to the stimulation of StAR. I am not aware of any other substance that can both inhibit aromatase (and long-lasting at that) AND promote endogenous testosterone/progesterone synthesis.

Two natural products, trans-phytol and (22E)-ergosta-6,9,22-triene-3β,5α,8α-triol, inhibit the biosynthesis of estrogen in human ovarian granulosa ... - PubMed - NCBI
"...Two compounds trans-phytol (SA-20) and (22E)-ergosta-6,9,22-triene-3β,5α,8α-triol (SA-48)-were found to potently inhibit estrogen biosynthesis (IC50: 1μM and 0.5μM, respectively). Both compounds decreased aromatase mRNA and protein expression levels in KGN cells, but had no effect on the aromatase catalytic activity in aromatase-overexpressing HEK293A cells and recombinant expressed aromatase. The two compounds decreased the expression of aromatase promoter I.3/II. Neither compound affected intracellular cyclic AMP (cAMP) levels, but they inhibited the phosphorylation or protein expression of cAMP response element-binding protein (CREB). The effects of these two compounds on extracellular regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinases (MAPKs), and AKT/phosphoinositide 3-kinase (PI3K) pathway were examined."
"...In the present study out of only 5 terpenoids and 6 steroids examined, one acyclic diterpenoid (SA-20), and one functionalized ergostane steroid, SA-48, potently inhibited estrogen biosynthesis in KGN cells. Currently, these two compounds are the most potent terpenoid and steroid reported to inhibit aromatase in a cell-based assay. Phytol (SA-20) is an acyclic diterpenoid categorized as a branched-chain fatty alcohol (3,7,11,15-tetramethyldexadec 2-en-1-ol). It is found abundantly in nature as part of the chlorophyll molecule, and a relatively high amount of free phytol is present in dairy products (Brown et al., 1993). The finding in this study that phytol is a potent aromatase inhibitor gives new insight on our understanding of the beneficial effects of vegetables and fruit on human health."
"...We found that SA-20 (phytol) and SA-48 exhibited their inhbitory effect in KGN cells until 12-24 h, indicating that they modulate aromatase at the transcriptional level. This is further supported by the findings that they inhibited aromatase mRNA and protein expression."
"...In normal breast cells, aromatase expression is primarily derived from the tissue-specific promoter I.4 for transcription, whereas in cells from patients with breast cancer, the expression is primarily derived from the utilization of promoter I.3/II. As a result, estrogen biosynthesis sqitches from regulation by a promoter controlled primarily by glucocorticoids and cytokines to regulation by a promoter controlled through cAMP-mediated pathways by prostaglandin E2 (PGE2), a powerful stimulator of adenylate cyclase (Zhao et al., 1996). Thus, the inhbition of promoter II-driven aromatase expression by means of anti inflammatory cyclooxygenase inhibitors to reduce PGE2 is attracting attention for the tissue specific treatment of breast cancer (Davies et al., 2012)."
"...Aromatase transcription is primarily controlled by promoter 1.3/II in ovarian granulosa cells. Thus, we examined whether SA-20 and SA-48 exert their inhibitory effects on aromatase transcription through these two promoters. SA-20 and SA-48 at 50uM decreased 20-30% of the promoter I.3 (Fig. 7A). However, at the same concentration (50uM), SA-20 and SA-48 decreased 60%-70% of the promoter II (Fig. 7B). These results indicate that the inhibition of aromatase transcription by SA-20 and SA-48 is mediated through promoter I.3/II, with promoter II playing a more prominent role."

In summary, phytol seems to be a very versatile substance with a wide array of beneficial effects. As one study aptly summarized these effects include:
Phytol in a pharma-medico-stance. - PubMed - NCBI
"...In the pharma-medico viewpoint, PYT and its derivatives have been evident to have antimicrobial, cytotoxic, antitumorous, antimutagenic, anti-teratogenic, antibiotic-chemotherapeutic, antidiabetic, lipid lowering, antispasmodic, anticonvulsant, antinociceptive, antioxidant, anti-inflammatory, anxiolytic, antidepressant, immunoadjuvancy, hair growth facilitator, hair fall defense and antidandruff activities. Otherwise, the important biometebolite of PYT is phytanic acid (PA). Evidence shows PA to have cytotoxic, anticancer, antidiabetic, lipid lowering and aniteratogenic activities. In addition, it may be considered as an important biomarker for some diseases such as Refsum's Disease (RD), Sjögren Larsson syndrome (SLS), rhizomelic chondrodysplasia punctata (RZCP), chronic polyneuropathy (CP), Zellweger's disease hyperpipecolic academia (ZDHA) and related diseases. Thus, phytol may be considered as a new drug candidate."

Finally, phytol is one of the few known chemicals that raise NAD levels in vivo and thus improve the oxidative state of the organism.
http://www.tandfonline.com/doi/pdf/10.1271/bbb.130029
"...In conclusion, we found that phytol increased the blood NAD level via ACMSD protein suppression and mRNA expression in rat liver. It is possible that this mechanism resulted from the activation of PPAR as well of other transcription factors. We will carry out additional studies on the regulation of ACMSD gene expression by phytol to elucidate this issue."


As the study on POH/GGOH observed, other related terpenes like farnesol or geranylgeranyl diphosphate (GGPP) were not effective in stimulating steroid synthesis, even though farnesol did boost progesterone synthesis but was much weaker compared to GGOH or POH.
"...We previously reported that menaquinone-4, one of vitamin K2, stimulates testosterone production in I-10 cells via regulation of PKA activity13) and presumed that unsaturated side chain of menaquinone-4, geranylgeranyl group, may be important to express this activity. And we also found that both menaquinone-4 and GGOH have anti-inflammatory activity in lipopolysaccaride-induced inflammation model.5,15) Here, we firstly demonstrated that GGOH stimulates steroidogenesis via activation of cAMP/PKA pathway. Testosterone production is regulated by a complex signaling cascade, with cholesterol transported into mitochondria to initiate steroidogenesis in Leydig cells. Progesterone is an important hormone that is converted to several steroids–including testosterone–and is secreted by I-10 cells.16,17) GGOH stimulated both testosterone and progesterone productions (Fig. 1). Therefore, we measured progesterone levels as a precursor of testosterone in further experiments in Figs. 3(B), 5, and 6(C) to clarify detailed mechanism of GGOH in steroidogenesis."

As the study describes, endogenous synthesis of steroids by the gonads (and actually peripheral cells as well) is rate-limited by the expression and activity of the so-called Steroid Acute Regulatory Protein (StAR).
Steroidogenic acute regulatory protein - Wikipedia

The higher the levels of this protein, the more steroids are synthesize from cholesterol and (maybe even more importantly) from other precursors as well. Cholesterol is not the only precursor to steroid synthesis and we are just beginning to understand the role of these other precursors. But as far as the levels of StAR, GGOH (and POH) increased those level more than 6-fold. I am not aware of any other substance with such dramatic effects on upregulating StAR. Finally, contrary to what other studies have suggested and what some forum members have asked for, PDE levels did not correlate with steroid synthesis. So, a PDE inhibitor will probably not be as effective as a steroid booster compared to GGOH or POH.
"...On the other hand, we did not find any evidence of a role for PDE in steroidogenesis. StAR functions in the rate-limiting step of steroid production in Leydig cells and is required for the delivery of cholesterol to the inner mitochondrial membrane.26) We showed that GGOH treatment increased the RNA and protein levels of StAR, which acts downstream in the cAMP/PKA pathway. Taken together, our results demonstrate that GGOH enhances testosterone and progesterone production in I-10 cells via induction of cAMP/PKA signaling. These findings provide novel mechanistic insight into the process of steroidogenesis and may be useful for the development of therapeutic strategies to counter age-associated declines in testosterone levels in men."

As you can see, the authors feel confident that the effects of POH and GGOH may be useful as therapy for declining levels of endogenous steroidogenesis in males. While the study was focused on males, there is evidence that the same steroidogenic process and rate-limiting steps are present in females. Thus, POH or GGOH should have similar beneficial effects in females which is supported by the fact that POH/GGOH boosted progesterone synthesis as much as testosterone. So, I am hoping that GGOH/POH could help both andropause and menopause.

Finally, Gonadin also contains diosgenin. It is most commonly extracted from the yam plant.
Diosgenin - Wikipedia

For decades, diosgenin has been widely used as a precursor for commercial steroid synthesis, especially of progesterone, cortisol and androgens. To my knowledge, nobody had specifically looked at its possible role as a steroid precursor when administered directly to mammals. As it turns out, a recent study found that administration of diosgenin dramatically increased DHEA and DHT levels in rodents and the increase of the steroids was seen in both serum and tissues. Serum levels of DHEA rose by a factor of more than 3 and serum DHT levels rose by about a factor of 2.

As a result of the increase in DHT, the blood glucose levels of the diabetic rats plummeted. This was confirmed by giving a 5-AR inhibitor to the rats given diosgenin, which prevented the blood glucose drop. What's even more interesting is that the effects were rather quick - the increase in DHEA and DHT happened only ~2 hours after giving a single relatively low dose of diosgenin (HED 0.5mg/kg). The structure of diosgenin is very similar to DHEA, and given DHEA's role as an efficient DHT precursor in humans, the study authors suspect diogenin is having the same effect. However, unlike DHEA, diosgenin is NOT known to convert into estrogen or otherwise raise estrogen levels.

Acute administration of diosgenin or dioscorea improves hyperglycemia with increases muscular steroidogenesis in STZ-induced type 1 diabetic rats. - PubMed - NCBI
"...Blood glucose level was significantly decreased 90–180 min after diosgenin injection, relative to the control group and diosgenin and 5-reductase inhibitor group. However, injection of diosgenin with the 5-reductase inhibitor suppressed the diosgenin-induced decrease in blood glucose level (Fig. 1A). Likewise, dioscorea injection also significantly decreased blood glucose level at 90–180 min after injection, compared to the control group, and at 150–180 min after injection compared to the 5-reductase inhibitor group, whereas injection of dioscorea with the 5- reductase inhibitor blocked the dioscorea-induced decrease in blood glucose level (Fig. 2A)."

"...Serum DHEA concentration was significantly increased 120 min after diosgenin injection, and 150 min after dioscorea injection (Fig. 2A). Serum DHT concentration was also significantly increased 150 min after diosgenin or dioscorea injection (Fig. 2B). Muscular DHEA concentrations were significantly greater in rats subjected to diosgenin injection and diosgenin injection with 5- reductase inhibitor than in the control rats (Fig. 3A). Muscular DHT concentrations were significantly greater in the diosgenin injection rats than in control rats. However, the DHT concentrations were significantly lower in the 5-reductase inhibitor rats than in the diosgenin injection rats (Fig. 3A). The muscular concentrations of DHEA and DHT in the muscle were confirmed, which corrected by per g tissue. The data was not different from the corrected value by mcg protein".

"...The results of this study showed that diosgenin and dioscorea administration induced a significant increase in serum DHEA level after 120 min; DHEA and DHT levels peaked after 150 min. In rats with type 1 diabetes mellitus, diosgenin and dioscorea administration decreased blood glucose level by about 28% and 21%, respectively. In our previous study, a 29% decrease in blood glucose level was seen 90 min after DHEA injection, compared to the baseline level [2]. The effect of diosgenin or dioscorea administration may be delayed relative to the effects of DHEA administration, because diosgenin and dioscorea must be converted to DHEA in vivo. In this study, administration of either diosgenin or dioscorea in conjunction with a 5-reductase inhibitor blocked the decrease of blood glucose level."

".... These activations are similar to DHEA administration [2]. Thus, diosgenin and dioscorea induced a decrease in blood glucose level, and activated the muscular GLUT4 signaling pathway, to the same degree as DHEA administration. We speculate that administration of diosgenin or dioscorea should also replenish sex steroid hormones and improve hyperglycemia in vivo. Although diosgenin and dioscorea administration increased serum sex steroid hormone levels in STZ-induced diabetes rats, it is still unclear where in the body the diosgenin and dioscorea are converted to DHEA. This issue should be addressed in a future study."

Interestingly, a human study showed that administration of just 8mg diosgenin improved cognitive function. This corroborates the DHEA- and DHT-raising effects of diosgenin as both of these androgens are known to improve memory and cognitive function.
Diosgenin-Rich Yam Extract Enhances Cognitive Function: A Placebo-Controlled, Randomized, Double-Blind, Crossover Study of Healthy Adults

Thus, in light of the evidence for beneficial effects of phytol, the fatty acid esters, and diosgenin I decided to release the product Gonadin. Its primary purpose is endogenous steroid optimization, however, it may be able to do much more judging from the studies in the "References" section below. The dose of phytol in Gonadin is based on the optimal phytol concentration (30 μM/L) from the in vitro study and the vitamin K2 study, as well as the subsequent in-vivo study with geranylgeraniol by the same authors. Thus, about 100mg is required to replicate the designs of all those studies. Like any unsaturated compounds, phytol has a potential for side effects. Animal studies have shown that at very high doses (10-15 times higher than doses in Gonadin), phytol can cause liver enlargement and its toxicity profile resembles that of vitamin A. So, it is probably best to stick to the 100mg phytol daily unless there is a very good reason for going with higher doses. To further lower the potential risk of such side effects related to phytol's unsaturated structure, Gonadin contains vitamin E. This addition is similar to the common practice of adding vitamin E to vitamin A (also unsaturated) supplements to both prevent peroxidation of the vitamin A and protect from its possible toxicities.

Gonadin is a chemical for optimizing endogenous steroid synthesis. One of its ingredients - phytol - has been shown in scientific studied to both promote the synthesis of steroids from endogenous precursors like cholesterol, as well as potentially inhibit aromatase and thus lower estrogen synthesis. Phytol has also been shown to possess a variety of other beneficial effects in animal (and some human) studies including restoring mitochondrial function in aged organisms, lowering cholesterol levels and triglycerides, protection from radiation-induced injury and immunosuppression, improvement of bile acid synthesis, antimicrobial, cytotoxic, antitumorous, antimutagenic, anti-teratogenic, antibiotic-chemotherapeutic, antidiabetic, lipid lowering, antispasmodic, anticonvulsant, antinociceptive, antioxidant, anti-inflammatory, anxiolytic, antidepressant, immunoadjuvancy, hair growth facilitator, hair fall defense and antidandruff activities, etc. Its other 3 ingredients - methyl palmitate, methyl oleate, and diosgenin - have been shown to increase androgens levels in both serum and muscle. Thus, the combination of the four (4) ingredients may help increase endogenous synthesis of androgens (and possibly progesterone as well) while simultaneously decreasing estrogen.

References:

1. Miscellaneous
Absorption and metabolic fate of dietary 3H-squalene in the rat. - PubMed - NCBI (squalene)
Dietary squalene increases tissue sterols and fecal bile acids in the rat. - PubMed - NCBI (squalene)
Metabolism of squalene in human fat cells. Demonstration of a two-pool system. - PubMed - NCBI (squalene)
Fate of intravenously administered squalene in the rat. - PubMed - NCBI (squalene)
Effects of prolactin, progesterone, and 17beta-hydroxy-5alpha-androstan-3-one on squalene production by the preputial gland of the immature female ... - PubMed - NCBI (squalene)
Squalene inhibits sodium arsenite-induced sister chromatid exchanges and micronuclei in Chinese hamster ovary-K1 cells. - PubMed - NCBI (squalene)
Biological importance and applications of squalene and squalane. - PubMed - NCBI (squalene)
Squalene as novel food factor. - PubMed - NCBI (squalene)
Squalene: A natural triterpene for use in disease management and therapy. - PubMed - NCBI (squalene)
The protective role of squalene in alcohol damage in the chick embryo retina. - PubMed - NCBI (squalene)
The inhibitory effects of squalene-derived triterpenes on protein phosphatase PP2A. - PubMed - NCBI (squalene)
Fate of intravenously administered squalene and plant sterols in human subjects. - PubMed - NCBI (squalene)
Feeding with supplemental squalene enhances the productive performance in boars. - PubMed - NCBI (squalene)
Squalene promotes the formation of non-bilayer structures in phospholipid model membranes. - PubMed - NCBI (squalene)
Postabsorptive metabolism of dietary squalene. - PubMed - NCBI (squalene)
Studies on the conversion of squalene to sterol with rat liver enzymes. - PubMed - NCBI (squalene)
Testicular sterols. VI. Incorporation of mevalonate into squalene and sterols by cell-free preparations of testicular tissue. - PubMed - NCBI (squalene)
Evaluation of Antioxidant Activity of Phytol Using Non- and Pre-Clinical Models. - PubMed - NCBI (phytol)
Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa. - PubMed - NCBI (phytol)
In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium. - PubMed - NCBI (phytol)
Phytol in a pharma-medico-stance. - PubMed - NCBI (phytol) (hair-growth effects)
In vitro anti-quorum sensing activity of phytol. - PubMed - NCBI (phytol)
Phytol derivatives as drug resistance reversal agents. - PubMed - NCBI (phytol)
Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical disease Schistosomiasis mansoni. - PubMed - NCBI (phytol)
Phytol metabolites are circulating dietary factors that activate the nuclear receptor RXR. - PubMed - NCBI (phytol)
Effects of dietary phytol and phytanic acid in animals. - PubMed - NCBI (phytol)
Effects of phytol, a branched, long-chain aliphatic alcohol, on biochemical values and on hepatic peroxisomal enzymes of rats. - PubMed - NCBI (phytol)


2. Metabolism/Mitochondria/Diabetes
Protective effect of dietary squalene supplementation on mitochondrial function in liver of aged rats. - PubMed - NCBI (squalene)
[SQUALENE: PHYSIOLOGICAL AND PHARMACOLOGICAL PROPERTIES]. - PubMed - NCBI (squalene)
Phytol/Phytanic acid and insulin resistance: potential role of phytanic acid proven by docking simulation and modulation of biochemical alterations. - PubMed - NCBI (phytol)
Phytanic acid, but not pristanic acid, mediates the positive effects of phytol derivatives on brown adipocyte differentiation. - PubMed - NCBI (phytol)
Effects of phytol, a branched, long-chain aliphatic alcohol, on biochemical values and on hepatic peroxisomal enzymes of rats. - PubMed - NCBI (phytol)
Phytol increases adipocyte number and glucose tolerance through activation of PI3K/Akt signaling pathway in mice fed high-fat and high-fructose diet. - PubMed - NCBI (phytol)

2. CVD/Cholesterol
Metabolic variables of cholesterol during squalene feeding in humans: comparison with cholestyramine treatment. - PubMed - NCBI (squalene)
Sterol synthesis from biliary squalene in the jejunal mucosa of the rat in vivo. - PubMed - NCBI (squalene)
The effect of the administration of squalene and other hydrocarbons on cholesterol metabolism in the rat (squalene)
Lipidaemic effects of tocotrienols, tocopherols and squalene: studies in the hamster. - PubMed - NCBI (squalene)
Effectiveness and safety of low-dose pravastatin and squalene, alone and in combination, in elderly patients with hypercholesterolemia. - PubMed - NCBI (squalene)
Dietary squalene increases high density lipoprotein-cholesterol and paraoxonase 1 and decreases oxidative stress in mice. - PubMed - NCBI (squalene)
Squalene ameliorates atherosclerotic lesions through the reduction of CD36 scavenger receptor expression in macrophages. - PubMed - NCBI (squalene)
Squalene in a sex-dependent manner modulates atherosclerotic lesion which correlates with hepatic fat content in apoE-knockout male mice. - PubMed - NCBI (squalene)
Cardioprotective effect of squalene on lipid profile in isoprenaline-induced myocardial infarction in rats. - PubMed - NCBI (squalene)
Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats. - PubMed - NCBI (squalene)
Amaranth squalene reduces serum and liver lipid levels in rats fed a cholesterol diet. - PubMed - NCBI (squalene)
Further in vitro evaluation of antiradical and antimicrobial activities of phytol. - PubMed - NCBI (phytol)


3. Inflammation
Dietary squalene supplementation improves DSS-induced acute colitis by downregulating p38 MAPK and NFkB signaling pathways. - PubMed - NCBI (squalene)
Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. - PubMed - NCBI (phytol)
Antinociceptive and Antioxidant Activities of Phytol In Vivo and In Vitro Models. - PubMed - NCBI (phytol)
Anti-scratching behavioral effect of the essential oil and phytol isolated from Artemisia princeps Pamp. in mice. - PubMed - NCBI (phytol)


4. Chemoprevention
Potentiation by squalene of the cytotoxicity of anticancer agents against cultured mammalian cells and murine tumor. - PubMed - NCBI (squalene)
Antitumor activity of squalene-treated cell-wall skeleton of Nocardia rubra in mice. - PubMed - NCBI (squalene)
Chemopreventive effect of squalene on colon cancer. - PubMed - NCBI (squalene)
The preventive and therapeutic potential of the squalene-containing compound, Roidex, on tumor promotion and regression. - PubMed - NCBI (squalene)
Inhibition by squalene of the tumor-promoting activity of 12-O-tetradecanoylphorbol-13-acetate in mouse-skin carcinogenesis. - PubMed - NCBI (squalene)
Modulation of doxorubicin-induced genotoxicity by squalene in Balb/c mice. - PubMed - NCBI (squalene)
Squalene: potential chemopreventive agent. - PubMed - NCBI (squalene)
The possible role of squalene as a protective agent in sebum. - PubMed - NCBI (squalene)
An insight into the cytotoxic activity of phytol at in vitro conditions. - PubMed - NCBI (phytol)
Diol- and triol-types of phytol induce apoptosis in lymphoid leukemia Molt 4B cells. - PubMed - NCBI (phytol)
Phytol induces programmed cell death in human lymphoid leukemia Molt 4B cells. - PubMed - NCBI (phytol)


5. Skin health
The possible role of squalene as a protective agent in sebum. - PubMed - NCBI (squalene)
High-dose squalene ingestion increases type I procollagen and decreases ultraviolet-induced DNA damage in human skin in vivo but is associated with... - PubMed - NCBI (squalene)
Squalene as a target molecule in skin hyperpigmentation caused by singlet oxygen. - PubMed - NCBI (squalene)
Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. - PubMed - NCBI (squalene)


6. Radiation Protection
Radioprotection of mice by dietary squalene. - PubMed - NCBI (squalene)


7. Neurotransmitters/Neuroprotection
Effects of squalene/squalane on dopamine levels, antioxidant enzyme activity, and fatty acid composition in the striatum of Parkinson's disease mou... - PubMed - NCBI (squalene)
Phytol a Natural Diterpenoid with Pharmacological Applications on Central Nervous System: A Review. - PubMed - NCBI (phytol)
Anxiolytic-like effects of phytol: possible involvement of GABAergic transmission. - PubMed - NCBI (phytol)
Anticonvulsant effect of phytol in a pilocarpine model in mice. - PubMed - NCBI (phytol)
 

Mr.Bollox

Member
Joined
Mar 16, 2021
Messages
3,171
Good stuff, any thoughts on why Ray doesnt like squalene, is it due to it being unsaturated or something? he mentioned it easily oxidizes
also wikipedia claims alpha-Naphthoflavone "has been shown to cause abnormal testicular development in young chickens" is this due to the powerful anti aromatase effects.
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
18,170
Location
USA / Europe
Good stuff, any thoughts on why Ray doesnt like squalene, is it due to it being unsaturated or something? he mentioned it easily oxidizes
also wikipedia claims alpha-Naphthoflavone "has been shown to cause abnormal testicular development in young chickens" is this due to the powerful anti aromatase effects.

Yeah, I think the easy oxidation is what he dislikes, and he also quoted some studies where they used squalene as a vaccine adjuvant, but that is not really relevant as it was injected and (I hope, at least) nobody is injecting Gonadin.
The Wikipedia page is simply lying. The study has no discussion of "abnormal development" as a result of supplementation. All it found is earlier maturation of sperm producing cells, massive increase in testicle size (the equivalent of a human getting balls the size of avocados) and as well as high T levels in the birds treated with ANF. No change in any other organ or signs of toxicity. See Figure 1 and Figure 4 of in the link below. Also, the dose used in that study was massive - equivalent to several grams per day for a human.
"...The drug vehicle consisted of a mixture of 20% gelatine (v/V), 20% olive oil, 30% glycerol and 30% H2O administered orally (syringe) at 1 ml/bird. Birds in MG3 and FG3 were fed the standard diet 5 days/wk and the standard diet + drug vehicle + α-naphthoflavone (60 mg/kg body weight) 2 days/wk."
"...With the exception of the testes of males supplemented with α-naphthoflavone, the oral administration of this flavonoid for 6 wks a�er hatching had no apparent effect on body or tissue growth until 12 wks of age, irrespective of the genetic sex. Testicular development in chickens is at first controlled by the photoperiod but factors such as genetic origin or feed allocation may also exert an influence (see Brillard, 2003 for review). Puberty, revealed by the presence of luminal spermatozoa in the seminiferous epithelium, can be observed as early as at 12 wks of age in males first subjected to a long photoperiod (e.g. from 16L:8D, de Reviers, 1971). By contrast, puberty is delayed by 4–6 wks in chicken males subjected to decreasing photoperiods (e.g. from 16L:8D to 8L:16D) (de Reviers, 1996). In the present study, neither the genetic origin (males from a fast growth rate broiler-type) nor feed allocation (ad libitum) can be considered as having exerted a retarding effect on gonad development. In addition, the photoperiod environment of these males was probably non-stimulatory according to the absence of spermiogenesis in the testes of males from the two control groups. It can therefore be postulated that the occurrence of qualitatively normal figures of spermiogenesis in 4 out of the 5 males treated with α-naphthoflavone was a direct consequence of the treatment rather than originating from interindividual variability of these males in response to the photoperiod."

Check out the original post (first page). I just updated it with the new information.
 

Mr.Bollox

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Yeah, I think the easy oxidation is what he dislikes, and he also quoted some studies where they used squalene as a vaccine adjuvant, but that is not really relevant as it was injected and (I hope, at least) nobody is injecting Gonadin.
The Wikipedia page is simply lying. The study has no discussion of "abnormal development" as a result of supplementation. All it found is earlier maturation of sperm producing cells, massive increase in testicle size (the equivalent of a human getting balls the size of avocados) and as well as high T levels in the birds treated with ANF. No change in any other organ or signs of toxicity. See Figure 1 and Figure 4 of in the link below. Also, the dose used in that study was massive - equivalent to several grams per day for a human.
"...The drug vehicle consisted of a mixture of 20% gelatine (v/V), 20% olive oil, 30% glycerol and 30% H2O administered orally (syringe) at 1 ml/bird. Birds in MG3 and FG3 were fed the standard diet 5 days/wk and the standard diet + drug vehicle + α-naphthoflavone (60 mg/kg body weight) 2 days/wk."
"...With the exception of the testes of males supplemented with α-naphthoflavone, the oral administration of this flavonoid for 6 wks a�er hatching had no apparent effect on body or tissue growth until 12 wks of age, irrespective of the genetic sex. Testicular development in chickens is at first controlled by the photoperiod but factors such as genetic origin or feed allocation may also exert an influence (see Brillard, 2003 for review). Puberty, revealed by the presence of luminal spermatozoa in the seminiferous epithelium, can be observed as early as at 12 wks of age in males first subjected to a long photoperiod (e.g. from 16L:8D, de Reviers, 1971). By contrast, puberty is delayed by 4–6 wks in chicken males subjected to decreasing photoperiods (e.g. from 16L:8D to 8L:16D) (de Reviers, 1996). In the present study, neither the genetic origin (males from a fast growth rate broiler-type) nor feed allocation (ad libitum) can be considered as having exerted a retarding effect on gonad development. In addition, the photoperiod environment of these males was probably non-stimulatory according to the absence of spermiogenesis in the testes of males from the two control groups. It can therefore be postulated that the occurrence of qualitatively normal figures of spermiogenesis in 4 out of the 5 males treated with α-naphthoflavone was a direct consequence of the treatment rather than originating from interindividual variability of these males in response to the photoperiod."

Check out the original post (first page). I just updated it with the new information.
i asked him about squalene once and he cited some studies showing involvement in blackheads, and said the claims of the cosmetic industry are as false as supplement industry. cosmetic products maybe use it as a moisturizer

but on other websites they mention squalene being an ancient molecule, say it has anti cancer properties
ah wow, they said abnormal but it seems it was abnormally good/virile
 

haidut

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i asked him about squalene once and he cited some studies showing involvement in blackheads, and said the claims of the cosmetic industry are as false as supplement industry. cosmetic products maybe use it as a moisturizer

but on other websites they mention squalene being an ancient molecule, say it has anti cancer properties
ah wow, they said abnormal but it seems it was abnormally good/virile

Yeah, I think he is just wary of anything the pharma industry has been using in its drugs, and squalene is one such chemical. It is naturally present in olive oil though, and if people are consuming 1tbsp olive oil daily as he mentioned in some podcasts, they would be ingesting several hundred milligrams of squalene - more than what was in the previous Gonadin formulation.
 

Mr.Bollox

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Yeah, I think he is just wary of anything the pharma industry has been using in its drugs, and squalene is one such chemical. It is naturally present in olive oil though, and if people are consuming 1tbsp olive oil daily as he mentioned in some podcasts, they would be ingesting several hundred milligrams of squalene - more than what was in the previous Gonadin formulation.
i heard humans produce it and it is a cholesterol precursor? if thats the case it should only be an issue if its oxidized in the supplement? Ray mentioned some concerns with oxidized cholesterol in things like dried milk powder, freeze dried liver, do you know what the issue is there?
also shark liver oil is like 30%+ squalene, 30%+ alkylglycerols, i think its one of the richest sources, not sure how much PUFA is in a gram of shark liver oil
 

haidut

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i heard humans produce it and it is a cholesterol precursor? if thats the case it should only be an issue if its oxidized in the supplement? Ray mentioned some concerns with oxidized cholesterol in things like dried milk powder, freeze dried liver, do you know what the issue is there?
also shark liver oil is like 30%+ squalene, 30%+ alkylglycerols, i think its one of the richest sources, not sure how much PUFA is in a gram of shark liver oil

Yes, we produce it as well in much higher quantities and is is present in sebum all over skin. Oxidation is the main problem, as well as when it is injected directly into the blood as they did with vaccines. Otherwise, in small amounts and applied orally/topically, I don't think it is an issue, especially when used together with vitamin E.
 
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