haidut

Member
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe
This study shows that depletion of NAD is a direct cause of the so-called hepatocellular carcinoma (HCC), which is the most common type of liver cancer. People with HCC often develop metastases in the pancreas and in advanced stages, the condition is indistinguishable from pancreatic cancer. Remarkably, not only did treatment with niacinamide completely prevent the development of HCC but it also made the established HCC tumors disappear. Niacinamide also fully protected from pancreatic cancer development. Unfortunately, the study did not look at whether niacinamide would also be able to cure established tumors like it did for HCC. The result of this study remind of another one where niacinamide completely prevented cancer metastases.
Niacinamide Fully Prevents Breast Cancer Metastasis

As far as doses - the HED was quite reasonable. The stated dose was 500 mg/kg of diet daily, which translates to 5.5mg/kg bodyweight daily for a human. Duration of treatment was 12 weeks.


https://www.sciencedaily.com/releases/2014/11/141120123234.htm
"...The inverse relationship between NAD+ and cancer awakened the curiosity of the researchers: could an increase in NAD+ have beneficial effects on the disease? When the scientists supplemented the diet in genetically modified mice with nicotinamide riboside, a derivative of vitamin B3 that increases intracellular levels of NAD+, they did not observe tumour development. Surprisingly, when they gave this diet to mice that had already developed the disease, the size of the tumours was reduced and they eventually disappeared."

http://www.cell.com/cancer-cell/fulltext/S1535-6108(14)00392-4
"...To investigate whether restoring NAD+ pools would prevent dysplastic nodules and tumor formation, 3-week-old hURI-tetOFFhep mice were supplied with a nicotinamide riboside (NR) diet. NR significantly increased hepatic NAD+concentrations (Figure S5A) without affecting liver-to-body weight ratio (Figure S5B). We detected dysplastic lesions and DNA damage in all mutants on chow, but not in those on NR, which also had reduced fibrosis, p53 abundance, and Ser-18 phosphorylation (Figures 5A–5D, S5C, and S5D). Prolonged NR treatment prevented tumor development and reduced ALT levels (Figures 5E–5G). Similarly liver tumors were prevented in 30-week-old homozygous mutants with higher URI levels (Figure S5E). Thus, restoring NAD+ pools protects from hURI-induced DNA damage, preneoplastic lesions, and tumor development. Surprisingly, 12-week-old homozygous mutants with full blown tumors then on 48 weeks of NR regimen showed significant tumor regression (Figures S5F and S5G), and their livers had high levels of cleaved caspase 3 (Figure S5H), suggesting that boosting NAD+ levels may be cytotoxic for tumor cells."

"...Next, we explored whether other oncogenes had similar effects. Ela-1-myc mice, unlike K-RasG12V mice, develop pancreatic adenocarcinomas with high levels of DNA damage, while pancreatic tumors initiated by K-RasG12V show no signs of replicative stress (Figures S5I and S5J). TOD2 and AFMID were clearly downregulated in Ela-1-myc, but not in K-RasG12Vpancreas (Figure S5K). In 3-week-old Ela-1-myc mice, 4 weeks of NR diet did not affect acinar-to-ductal metaplasia (ADM), but 12 weeks of NR diet decreased ADM and carcinomas formation compared to chow fed mice (Figures S5L–S5O). Importantly pancreatic NAD+ levels were significantly reduced in Ela-1-myc mutants on chow diet, but restored to almost control levels on NR diet (Figure S5P). Thus, oncogene-induced DNA damage has a common bearing on NAD+ levels."
 
Last edited:

Nick Nieves

Member
Joined
Aug 19, 2015
Messages
13
How different is nicotinamide riboside from niacinamide? Is it just more effective at raising NAD+ at lower doses?

Also, are you planning on releasing a pure niacinamide supplement?
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe
How different is nicotinamide riboside from niacinamide? Is it just more effective at raising NAD+ at lower doses?

Also, are you planning on releasing a pure niacinamide supplement?

Both niacinamide and NR have to go through the same intermediate called nicotinamide mononucleotide in order to raise NAD levels (see attached image). They have comparable effectiveness in raising NAD, but there is evidence that NR may actually be worse than niacinamide since not only does NR raise NAD levels it also increases NAD consumption through PARP (see study below). This is not desirable as the net effect on long term NAD levels may be nil or even negative. In addition, NR is an activator of sirtuins (SIRT) while niacinamide is a SIRT inhibitor and it also inhibits NAD consumption. The inhbition of NAD consumption together with inhbition of sirtuins is currently in clinical trials for treating cancer.
Nicotinamide riboside is uniquely and orally bioavailable in mice and humans : Nature Communications
"...In addition to its role as a coenzyme, NAD+ is the consumed substrate of enzymes such as poly-ADPribose polymerases (PARPs), sirtuins and cyclic ADPribose synthetases1. In redox reactions, the biosynthetic structures of NAD+, NADH, NADP+ and NADPH are preserved. In contrast, PARP3, sirtuin4 and cyclic ADPribose synthetase5activities hydrolyze the linkage between the nicotinamide (Nam) and the ADPribosyl moieties of NAD+ to signal DNA damage, alter gene expression, control post-translational modifications and regulate calcium signalling."

"...Nam without conversion to NAD+. Interestingly, of three NAD+precursor vitamins provided in bolus at equivalent oral doses, Nam provided the least increase in ADPR (Fig. 5j). Whereas the area under the curve (AUC) of the Nam-driven rise in hepatic NAD+ indicated a ∼50% advantage of Nam over NA (Fig. 5b), there was a >50% deficit in Nam-driven ADPR accumulation versus NA (Fig. 5j). This is consistent with the idea that high-dose NA, though not an ideal hepatic NAD+precursor, is effective as a cholesterol agent whereas Nam is not44because high-dose Nam inhibits sirtuins1. Notably, NR is active as a cholesterol-lowering agent in overfed mice30."
 

Attachments

  • nam.jpg
    nam.jpg
    13.8 KB · Views: 76
Last edited:

superhuman

Member
Joined
May 31, 2013
Messages
1,124
Interesting. I thought esp high dose niacinamide would increase liver enzymes and cause problems?
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe
Interesting. I thought esp high dose niacinamide would increase liver enzymes and cause problems?

You'd have to take more than 3g daily for months to get this effect and even then it is not clear that the niacinamide was toxic as it only elevated liver enzymez to less than 2x the upper limit and there was no evidence of tissue pathology. Broda Barnes said that this elevation of liver enzymes to less than 2x upper limit is actually a sign of liver working well and not a sign of liver injury. The dose used in this study was <500mg daily for most people and in those doses niacinamide was shown to reverse liver damage.
Low Dose Niacinamide Prevents NAFLD / Cirrhosis
 

superhuman

Member
Joined
May 31, 2013
Messages
1,124
You'd have to take more than 3g daily for months to get this effect and even then it is not clear that the niacinamide was toxic as it only elevated liver enzymez to less than 2x the upper limit and there was no evidence of tissue pathology. Broda Barnes said that this elevation of liver enzymes to less than 2x upper limit is actually a sign of liver working well and not a sign of liver injury. The dose used in this study was <500mg daily for most people and in those doses niacinamide was shown to reverse liver damage.
Low Dose Niacinamide Prevents NAFLD / Cirrhosis

Yeah thats great. Im currently only doing 50mg 3x a day so maybe i should try and increase it since i have some signs of a slower or lower then optimal liver and kidney function
 

chispas

Member
Joined
Dec 4, 2014
Messages
354
You'd have to take more than 3g daily for months to get this effect and even then it is not clear that the niacinamide was toxic as it only elevated liver enzymez to less than 2x the upper limit and there was no evidence of tissue pathology. Broda Barnes said that this elevation of liver enzymes to less than 2x upper limit is actually a sign of liver working well and not a sign of liver injury. The dose used in this study was <500mg daily for most people and in those doses niacinamide was shown to reverse liver damage.
Low Dose Niacinamide Prevents NAFLD / Cirrhosis

This is exactly the protocol I suggested to a friend after being told that she needed surgery for NAFL disease. She had ultrasounds done that demonstrated the extent of the problem. After six months of taking 500mg B3 a day (the pain had vanished within two weeks of commencing the dose) she had a follow up scan, which showed nothing but a healthy liver, and this was reflected in her blood tests, which normalised. The specialist was angry that she used B3, and said it was quackery. The specialist had put her on a diet low in sugar, and recommended she stop drinking Coke. He must have been applying the "try to kill the patient" treatment!

She still takes B3 today as a preventative because she consumes a lot of oily fish as part of her traditional islander diet. She continues to have no problems, and no longer eats a low sugar diet.
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe
This is exactly the protocol I suggested to a friend after being told that she needed surgery for NAFL disease. She had ultrasounds done that demonstrated the extent of the problem. After six months of taking 500mg B3 a day (the pain had vanished within two weeks of commencing the dose) she had a follow up scan, which showed nothing but a healthy liver, and this was reflected in her blood tests, which normalised. The specialist was angry that she used B3, and said it was quackery. The specialist had put her on a diet low in sugar, and recommended she stop drinking Coke. He must have been applying the "try to kill the patient" treatment!

She still takes B3 today as a preventative because she consumes a lot of oily fish as part of her traditional islander diet. She continues to have no problems, and no longer eats a low sugar diet.

If B3 was quackery than what was the disappearance of the NALF? Divine intervention?
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe

chispas

Member
Joined
Dec 4, 2014
Messages
354
If B3 was quackery than what was the disappearance of the NALF? Divine intervention?

You see, the disappearance of the NAFL correlated precisely to the disappearance of a repayment the specialist hoped to make towards his luxury car. There's no way those two phenomena are linked, so quackery must be responsible!
 

MigFon

Member
Joined
Oct 31, 2015
Messages
53
I've had an 11 mm nodule disappear from my liver last year, either HCC or a metastasis from testicular cancer in 2015. 4 g of niacinamide daily was part of the protocol.
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe
Does niacinamide have a crystalline structure? Whenever I spill the powder it reminds me of fine fiber glass

I think it does. When I dissolved in DMSO and leave out in the cold it forms crystals inside the bottle. Other supplements simply precipitate as a cloudy residue, but niacinamide does not.
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe
What's the ideal dosage to skyrocket that GABA?

What does GABA have to do with the study above? Niacinamide does not raise GABA as far as I know, it acts as GABAa agonist.
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,944
Location
USA / Europe
I've had an 11 mm nodule disappear from my liver last year, either HCC or a metastasis from testicular cancer in 2015. 4 g of niacinamide daily was part of the protocol.

Amazing! What else were you taking? Vitamin K and caffeine maybe?
 

MigFon

Member
Joined
Oct 31, 2015
Messages
53
Amazing! What else were you taking? Vitamin K and caffeine maybe?
I took about 4g of Aspirin, 4 g niacinamide, 30drops of lapodin 3x per day, 1 grain of tyromax, 15 mg of kuinone 3x per day, 40 drops mitolipin, 20 mg progesterone 2x per day, Vitamins A, D, E, some Methylene Blue and some Turkey Tail Mushroom supplement, and I think that was about it.

As far as caffeine is concerned, I brewed some really strong coffee and tried to drink as much as I could (up to 300mL, but it varied a lot from day to day), however, coffee/caffeine supplements really upset my intestines and I think my glycogen stores suck, so I was really limited on the amount an how often I could drink.

Perhaps some of the supplementation doesn't make much sense, but as soon as I found out about the nodule I tried to soak up as much information from this forum as possible, and this protocol was the best I could come up with on such short notice.

I don't know what did it, but something seemed to have worked.

I would like to thank you personally because your posts were invaluable, as well as the idealabs supplements.

Thank you very much Sir.
 
Last edited:

High_Prob

Member
Joined
Mar 23, 2016
Messages
326
What does GABA have to do with the study above? Niacinamide does not raise GABA as far as I know, it acts as GABAa agonist.

I am confused: Isn't agonizing Gaba the same as increasing it? (I promise this will not become a Gaba thread, this just threw me off)
 
Similar threads
Thread starter Title Forum Replies Date
haidut Niacinamide (vitamin B3) May Cure HIV (human Study) Scientific Studies 10
D 100mg allithiamine (+niacinamide&magnesium) makes me hot and sweating Supplements, Pharmaceutical Drugs 4
J Huge improvements with niacinamide Water-Soluble Vitamins 0
J Niacinamide Ask For Help or Advice 14
haidut Niacinamide increases aerobic capacity, muscle endurance in humans Scientific Studies 1
M Strange effect from niacinamide Supplements, Pharmaceutical Drugs 11
F What was your niacinamide experience? Ask For Help or Advice 2
B Berberine Vs. Niacinamide Ask For Help or Advice 5
O Microdosing Niacinamide Supplements, Pharmaceutical Drugs 3
forterpride Niacinamide dosing for diabetes Ask For Help or Advice 48
wealthofwisdom Niacinamide & food timing? Ask For Help or Advice 9
E Coke + niacinamide + glycine Ask For Help or Advice 3
ddjd Taurine causes nerve pain issues. Niacinamide heals. What's the mechanism?? Ask For Help or Advice 18
C Whats the verdict on Niacin/Niacinamide? Supplements, Pharmaceutical Drugs 31
IROM Alpecin shampoo: Caffeine, Taurine and Niacinamide (are corporations on to Peating?) Hair & Nails 9
youngsinatra High Dose Niacinamide Self-Experiment. Experiments 32
haidut Glaucoma likely a metabolic disease, treatable by pyruvate and/or niacinamide Scientific Studies 2
haidut Niacinamide may treat ALS by improving energetic state Scientific Studies 0
haidut Niacinamide may treat depression by improving energetic state Scientific Studies 8
S Aspirin, niacinamide and eye bags Supplements, Pharmaceutical Drugs 7
J Niacinamide Ask For Help or Advice 15
SOMO Niacinamide + Vit C = Niacin? Ask For Help or Advice 0
Razvan Niacinamide Help Ask For Help or Advice 1
pauljacob Can I Break Open A Niacinamide, Pregnenolone Capsule? Supplements, Pharmaceutical Drugs 4
charlie Vitamin B3 (Niacinamide) Shows Promise For Glaucoma Patients In Clinical Trial Scientific Studies 2
snacks Niacinamide And Dandruff Skin 2
ddjd Niacinamide Great For Sleep. Niacin Terrible. Why? Ask For Help or Advice 10
Hgreen56 Niacinamide Inhibits Fatloss And Niacin Improve It? Supplements, Pharmaceutical Drugs 10
ampersand Ataxia, Iron, Methylene Blue, Niacinamide Supplements, Pharmaceutical Drugs 2
haidut Niacinamide May Treat Fibrotic Eye Disease And Prevent Vision Loss Scientific Studies 5
C Aspirin/Niacinamide/Pyrucet Supplements, Pharmaceutical Drugs 7
meatbag Has Anyone Ever Taken 5g+ Niacinamide In One Sitting? Experiments 20
Orangeyouglad Any Ideas On Tolerating Niacinamide? Supplements, Pharmaceutical Drugs 5
haidut Low-dose (but Not High) Niacinamide Reverses Reproductive Decline In Old Females Scientific Studies 6
W Niacinamide And Depression B3/Niacinamide 19
G Niacinamide Long Term Use Cortisol, Serotonin, Histamine 16
Ficini Niacinamide Topical Vs Oral Clarification B3/Niacinamide 2
haidut Dietary Niacinamide (B3) Deficiency May Be A Cause Of Schizophrenia Scientific Studies 7
SOMO Niacinamide Causing Skin Issues For Anyone Else? Supplements, Pharmaceutical Drugs 4
Batbat Niacinamide Inhibits Hair Growth B3/Niacinamide 7
haidut Niacinamide May Restore Female Fertility In Old Age Scientific Studies 17
Jewbermensch Niagen Worth It Over Niacinamide? Supplements, Pharmaceutical Drugs 22
haidut Niacinamide May Improve Symptoms Of ALS Scientific Studies 1
E Issues After Taking High Dose Niacinamide Supplements, Pharmaceutical Drugs 20
Inaut I Think I’ve Finally Figured Out How To Use Niacinamide B3/Niacinamide 90
T How Much Aspirin And Niacinamide? Female 44 Yrs Fatigue And Heavy Menstruation Supplements, Pharmaceutical Drugs 5
haidut Niacinamide May Help Treat/reverse Heart Disease Scientific Studies 1
A Niacin NOT The Same As Niacinamide, Acts Differently Regarding Lipolysis? Vitamins 13
Amazigh Niacinamide + Methyl Donor To Avoid Histamine Increase? Supplements, Pharmaceutical Drugs 6
olive Nicotinamide (niacinamide) Induces Liver Regeneration & Improves Liver Function By Activating SIRT1 Scientific Studies 52

Similar threads

Top