http://www.sciencedirect.com.proxy1.athensams.net/science/article/pii/S0010782402005152
>>Methylene blue was the second most potent sperm-immobilizing agent from the series of compounds studied. As a vasography stain, its deleterious effect on sperm motility has been reported in comparison to other contrast dyes such as indigo carmine and Renografin® [11]. The fact that methylene blue is commonly used as a vasography dye attests to its safety as a potential vas irrigant.<<
>>Spermatozoa are highly susceptible to reactive oxygen species (ROS) [26], [27] and [28], and methylene blue has the capability to produce ROS by transferring electron equivalents from reducing substances to molecular oxygen [29]. In this process, it appears to form unstable, highly reactive organoradical intermediates [30], which might be directly responsible for its sperm-immobilizing activity [31].<<
>>Methylene blue was the second most potent sperm-immobilizing agent from the series of compounds studied. As a vasography stain, its deleterious effect on sperm motility has been reported in comparison to other contrast dyes such as indigo carmine and Renografin® [11]. The fact that methylene blue is commonly used as a vasography dye attests to its safety as a potential vas irrigant.<<
>>Spermatozoa are highly susceptible to reactive oxygen species (ROS) [26], [27] and [28], and methylene blue has the capability to produce ROS by transferring electron equivalents from reducing substances to molecular oxygen [29]. In this process, it appears to form unstable, highly reactive organoradical intermediates [30], which might be directly responsible for its sperm-immobilizing activity [31].<<