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This study shows that combing light therapy, methylene blue and potassium iodide is very effective against infections and pathogenic microbes.
Antimicrobial photodynamic therapy mediated by methylene blue and potassium iodide to treat urinary tract infection in a female rat model
"Antimicrobial photodynamic therapy (aPDT), a specific form of PDT in general, is the term used to describe the combination of non-toxic dyes called photosensitzers (PS) and light that in the presence of oxygen produces highly reactive oxygen species (ROS) such as singlet oxygen (1O2, Type II photochemical mechanism) and hydroxyl radicals (HO·, Type I photochemical mechanism)20. These ROS can damage biomolecules (proteins, lipids, nucleic acids) in a wide range of microorganisms regardless of structure or drug resistance and produce rapid killing of many logs of cells. If light is delivered soon after introduction of PS into infected tissue, significant selectivity for microbial cells over host cells is achieved21. We have reported that aPDT mediated by a range of different PS can be strongly potentiated by addition of the non-toxic inorganic salt, potassium iodide"
So potassium iodide is not needed by will strongly amplify the effect.
"We originally hypothesized that the mechanism of action involved one-electron transfer to iodide anion to produce iodine radicals (Type I), but subsequent studies showed that iodide underwent an addition reaction to singlet oxygen (Type II) to produce reactive iodine species and hydrogen peroxide22 also producing the stable antimicrobial substance, iodine/tri-iodide. We also demonstrated that iodide potentiation of aPDT could be demonstrated in vivo"
"We also showed that MB combined with KI, excited with red laser was effective treatment in a mouse model of oral candidiasis"
0.5 mL of MB solution was instilled into the bladder via catheter for 15 mins before light irradiation and then after MB instillation, bladders were then instilled with a 0.5 mL aliquot of 100 mM KI solution.
For irradiation, the 660 nm laser was coupled into a fused plastic fiber (core diameter 500 µm) with a glass cylindrical diffusing tip (diameter 0.98 mm, length 10 mm). The tube was also inserted into the rat, so it wasn't red light on the skin.
So although the solutions and red light was given specifically to that area internally, I think higher oral doses of MB and KI would also work to saturate the tissue before red light exposure. 5-15mg methylene blue would probably be enough for this effect if taken over a few days to saturate the tissue.
I think direct red light/sunlight to that area would also work if MB and KI is taken before exposure, with about 30 minutes exposure to be adequate.
This strategy can probably be used to kill any bacteria or infection in the body. As it creates ROS, maybe this would be a novel way to kill tumor and cancer cells as well.
@Obi-wan
Anti-viral/inflammatory treatment also inhibits iron retention and can allow the body to use iron correctly and to eliminate it as needed.
Antimicrobial photodynamic therapy mediated by methylene blue and potassium iodide to treat urinary tract infection in a female rat model
"Antimicrobial photodynamic therapy (aPDT), a specific form of PDT in general, is the term used to describe the combination of non-toxic dyes called photosensitzers (PS) and light that in the presence of oxygen produces highly reactive oxygen species (ROS) such as singlet oxygen (1O2, Type II photochemical mechanism) and hydroxyl radicals (HO·, Type I photochemical mechanism)20. These ROS can damage biomolecules (proteins, lipids, nucleic acids) in a wide range of microorganisms regardless of structure or drug resistance and produce rapid killing of many logs of cells. If light is delivered soon after introduction of PS into infected tissue, significant selectivity for microbial cells over host cells is achieved21. We have reported that aPDT mediated by a range of different PS can be strongly potentiated by addition of the non-toxic inorganic salt, potassium iodide"
So potassium iodide is not needed by will strongly amplify the effect.
"We originally hypothesized that the mechanism of action involved one-electron transfer to iodide anion to produce iodine radicals (Type I), but subsequent studies showed that iodide underwent an addition reaction to singlet oxygen (Type II) to produce reactive iodine species and hydrogen peroxide22 also producing the stable antimicrobial substance, iodine/tri-iodide. We also demonstrated that iodide potentiation of aPDT could be demonstrated in vivo"
"We also showed that MB combined with KI, excited with red laser was effective treatment in a mouse model of oral candidiasis"
0.5 mL of MB solution was instilled into the bladder via catheter for 15 mins before light irradiation and then after MB instillation, bladders were then instilled with a 0.5 mL aliquot of 100 mM KI solution.
For irradiation, the 660 nm laser was coupled into a fused plastic fiber (core diameter 500 µm) with a glass cylindrical diffusing tip (diameter 0.98 mm, length 10 mm). The tube was also inserted into the rat, so it wasn't red light on the skin.
So although the solutions and red light was given specifically to that area internally, I think higher oral doses of MB and KI would also work to saturate the tissue before red light exposure. 5-15mg methylene blue would probably be enough for this effect if taken over a few days to saturate the tissue.
I think direct red light/sunlight to that area would also work if MB and KI is taken before exposure, with about 30 minutes exposure to be adequate.
This strategy can probably be used to kill any bacteria or infection in the body. As it creates ROS, maybe this would be a novel way to kill tumor and cancer cells as well.
@Obi-wan
Anti-viral/inflammatory treatment also inhibits iron retention and can allow the body to use iron correctly and to eliminate it as needed.
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