tankasnowgod
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- Jan 25, 2014
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I have been taking cyproheptadine again as a self experiment, in higher doses that when I previously used it. Usually, I am taking 4mg with every meal. After a few days, I started to notice I would often get very warm after a meal, especially in the face and chest area near my thyroid. It doesn't happen all the time, but it's happened frequently enough that I was starting to wonder if cyproheptadine had any uncoupling effects. After some searching, I did find one in vitro study (only a page long) that suggests just that-
I ran the numbers through Sigma Aldrich's Molarity calculator, and it seems like a human might be able to hit the low end of the range with a 5mg dose (or lower if it's already in the body from chronic use). My calculations could be off, and since I was using the lowest molarity (3.3 μM), I didn't use a HED, just what was in the study. Also, I know there can be many issues estimating an in vivo dose from an in vitro dose, and I don't know how to control for that. If anyone would like to check my work, please do.
The Effect of Cyproheptadine on Oxidative Phosphorylation in Rat Liver Mitochondria
I Cameron, S J Jones, A Markham, R M Morgan, F Whitfield; The Effect of Cyproheptadine on Oxidative Phosphorylation in Rat Liver Mitochondria, Journal of P
academic.oup.com
Cyproheptadine 3.33-100μM was found to produce a concentration-dependent stimulation of State 4 respiration (substrate and oxygen in excess, ADP absent) during the NAD+-linked oxidation of 5mM glutamate plus 5mM malate by tightly coupled rat hepatic mitochondria, with the rate of respiration increasing from 22.6 f 1.0 to 47.4 f 4.0 ng atoms 02 consumed min-lmg of protein-l (n=4) , resulting in an EC50 value of 28.2pM.
Cyproheptadine was shown to cause a concentration-dependent stimulation of mitochondrial ATPase (E.C.3.6.1.4) activity over the same concentration range that produced a stimulation of State 4 respiration; with the rate of activity increasing from 4.87 f 0.39 to 10.84 f 1.34 nmoles inorganic PO4 released min-lmg of protein-l (n=4). A similar but significantly (pt0.05) more potent effect was observed with DNP (1-1OOpl) with the rate increasing from 4.90 f 0.53 to 16.05 f 0.87 nmoles inorganic PO4 released min-lmg of protein-' (n=4).
I ran the numbers through Sigma Aldrich's Molarity calculator, and it seems like a human might be able to hit the low end of the range with a 5mg dose (or lower if it's already in the body from chronic use). My calculations could be off, and since I was using the lowest molarity (3.3 μM), I didn't use a HED, just what was in the study. Also, I know there can be many issues estimating an in vivo dose from an in vitro dose, and I don't know how to control for that. If anyone would like to check my work, please do.