• Due to excessive bot signups along with nefarious actors we are limiting forum registration. Keep checking back for the register link to appear. Please do not send emails or have someone post to the forum asking for a signup link. Until the current climate changes we do not see a change of this policy. To join the forum you must have a compelling reason. Letting us know what skills/knowledge you will bring to the community along with the intent of your stay here will help in getting you approved.

Restoring oxidative metabolism has curative effects on cancer (leukemia)


Mar 18, 2013
USA / Europe
A great study, which directly demonstrates not only the OXPHOS deficiency characteristic of all "cancer" cells, but also proving that restoration of OXPHOS is highly therapeutic (and likely curative) for leukemia. Just as importantly, the study demonstrates that cancer cells have, in fact, higher respiration rats than normal cells, however the mitochondria of cancer cells consume rather than produce ATP. The source of that ATP is likely the upregulated glycolysis (Warburg Effect), which is another hallmark of all cancer cells. So, in a sense, cancer cells have a very high rate of "futile" metabolism and they consume ATP in order to grow and multiply, instead of contributing to the healthy (OXPHOS vs. glycolysis) process of producing energy. Cancer, it seems, is an energetic parasite of sorts. While the study was done on leukemia cells, the authors discuss that the same metabolic phenotype has been demonstrated in every other type of cancer cell studied, and as such their findings are likely applicable to all other cancers, hematological or solid alike. The study used several chemicals as interventions to restore OXPHOS, and one of them was the naturally occurring chemical curcumin. However, since the OXPHOS deficiency (which is a requirement for cancer cell survival/growth) was found to be due to inhibition of electron transport chain (ETC) Complex II/III/IV (one of more of them) this means that all other remedies that target those portions of the ETC will likely also be therapeutic/curative. Many quinone molecules are known to restore the function of those ETC segments, which provides a plausible explanation of why the tetracycline family of antibiotics (also quinones) has been known to induce immediate remission in virtually all blood cancers studied. If those antibiotics are not available then other quinones such as methylene blue, vitamin K, emodin, CoQ10, etc may also be able to provide the same effects.
Finally, while the study did not look at the cause of reduced ETC/OXPHOS functionality in cancer cells, I'd venture a guess that is due to the increased fatty acid oxidation (FAO) in cancer, which lowers the mitochondrial NAD/NADH ratio, which ultimately inhibits the flow of electrons along the entire ETC. Thus, simply reducing FAO should also have broad therapeutic (and even curative) effects, as has already been demonstrated by other studies posted on my blog in the past.

In Vitro Evaluation of the Antiviral Activity of Methylene Blue Alone or in Combination against SARS-CoV-2 - PubMed
"...Currently there is great interest in targeting mitochondrial oxidative phosphorylation (OXPHOS) in cancer. However, notwithstanding the targeting of mutant dehydrogenases, nearly all hopeful 'mito-therapeutics' cannot discriminate cancerous from non-cancerous OXPHOS and thus suffer from a limited therapeutic index. Using acute myeloid leukemia (AML) as a model, herein, we leveraged an in-house diagnostic biochemical workflow to identify 'actionable' bioenergetic vulnerabilities intrinsic to cancerous mitochondria. Consistent with prior reports, AML growth and proliferation was associated with a hyper-metabolic phenotype which included increases in basal and maximal respiration. However, despite having nearly 2-fold more mitochondria per cell, clonally expanding hematopoietic stem cells, leukemic blasts, as well as chemoresistant AML were all consistently hallmarked by intrinsic OXPHOS limitations. Remarkably, by performing experiments across a physiological span of ATP free energy, we provide direct evidence that leukemic mitochondria are particularly poised to consume ATP. Relevant to AML biology, acute restoration of oxidative ATP synthesis proved highly cytotoxic to leukemic blasts, suggesting that active OXPHOS repression supports aggressive disease dissemination in AML. Together, these findings argue against ATP being the primary output of leukemic mitochondria and provide proof-of-principle that restoring, rather than disrupting, OXPHOS may represent an untapped therapeutic avenue for combatting hematological malignancy and chemoresistance."

"...Increased mitochondrial oxidative metabolism, an established metabolic hallmark of leukemia (Byrd et al., 2013; Kuntz et al., 2017; Lee et al., 2015; Sriskanthadevan et al., 2015; Suganuma et al., 2010), has been historically interpreted to reflect an increased reliance on mitochondrial ATP production. However, fractional OXPHOS kinetics had not been empirically evaluated in leukemia at the onset of this project. Thus, it remained to be determined whether higher basal respiration in leukemia reflected accelerated demand for ATP regeneration or intrinsic OXPHOS insufficiency. Both conditions would be expected to similarly restrict cellular ATP/ADP equilibrium displacement (i.e. ΔGATP charge) and thus could potentially result in identical respiratory profiles in intact cells. For example, a small network of mitochondria each respiring near maximal capacity could in theory produce an identical ‘basal’ oxygen consumption rate to that of a comparatively larger mitochondrial network in which forward respiratory flux was constrained across each mitochondrial unit. Our findings provide definitive support for the latter scenario in AML, as application of our diagnostic biochemical workflow revealed that intrinsic limitations in fractional OXPHOS characterize an expansive mitochondrial network in human leukemia. In fact, a substantial portion of the AML mitochondrial network is incapable of contributing to oxidative ATP production, as leukemic mitochondria primarily consume, rather than produce, ATP across a physiological ΔGATP span. Intrinsic OXPHOS limitations in AML appear to derive from a unique biochemical mechanism whereby extra-mitochondrial ATP gains access to the matrix space, where it then directly inhibits electron transport flux in a ΔGATP-dependent manner. Such inhibition is independent of ATP synthase (i.e. CV) and presumably localized to the respiratory complexes downstream of the ubiquinone pool (i.e. CIII, Cyt C, CIV). Given that evidence for this effect was also observed in bone marrow-derived CD34+ stem cells, allosteric and/or post-translation regulation of ETS flux is likely a primary mode of OXPHOS regulation in hematopoietic progenitors that is maintained during leukemogenesis. Importantly, reversal of this effect was strongly cytotoxic to AML, indicating that direct OXPHOS regulation by ΔGATP confers a survival advantage during hematopoietic clonal cell expansion. Although additional work will be required to fully elucidate the mechanism(s) by which ATP uptake directly inhibits OXPHOS flux in AML, our preliminary findings leveraging gamitrinib and curcumin provide proof-of-principle that such regulation can indeed be targeted therapeutically."


May 16, 2016
What about Mildronate in the context of the study? Seems it might work quite well too.


Feb 3, 2020
Frankfurt, Germany
I am still trying to come up with a stack that covers (nearly) all complexes and sub-steps of oxidative energy metabolism.

I personally use a blend of thiamin HCL, riboflavin, niacinamide, biotin, ubiquinone, magnesium, vitamin E, topical D3/K2 + dietary calcium, nutrient-dense animal proteins, collagen, salt, carbohydrates and coffee.

I think methylene blue, cascara, tetracyclines are good candidates too.

Anything you’d potentially add or change?
Similar threads
Thread starter Title Forum Replies Date
milkboi 1g of Dopamine Restoring 9-Me-BC for sale (Europe) Supplements, Pharmaceutical Drugs 4
haidut Restoring Glucose Metabolism May Treat Crohn's Disease (CD) Scientific Studies 8
haidut Restoring Metabolism Prevents / Reverses "permanent" Nerve Damage Scientific Studies 29
haidut Restoring Energy Production In Immune Cells Is Therapeutic In Cancer Scientific Studies 2
Atma Return To The Brain Of Eden: Restoring The Connection Between Neurochemistry And Consciousness The Ray Peat Forum Lounge 5
shine Choline Supplementation Alleviates Fluoride-induced Testicular Toxicity By Restoring The NGF And MEK Scientific Studies 1
haidut Aspirin May Treat Tuberculosis By Improving / Restoring Innate Immunity Scientific Studies 3
lvysaur Restoring The Small Intestinal Membrane Cures Diabetes Articles & Scientific Studies 37
H Intermittent Fasting Has Positive Effect On Restoring Diurnal Cycles Of Gut Microbiome Scientific Studies 4
Mito Reversing Wrinkled Skin And Hair Loss In Mice By Restoring Mitochondrial Function Scientific Studies 31
Ashoka Quitting Smoking And Restoring Lung Function? Ask For Help or Advice 4
T Email Peat Quote RE Restoring Metabolic Health Email Advice 5
cb4 Restoring Tooth Enamel Oral Health 29
natedawggh TAURINE The Key To Restoring Metabolic Function? Taurine 144
Velve921 Restoring Levels Of Magnesium Magnesium 49
aliml Role of Human NADPH Quinone Oxidoreductase (NQO1) in Oxygen-Mediated Cellular Injury and Oxidative DNA Damage in Human Pulmonary Cells Articles & Scientific Studies 4
Broken man Methylene Blue decreases mitochondrial oxidative stress and alleviates cardiac dysfunction in diabetes Scientific Studies 14
haidut Cartilage/bone health depends on oxidative metabolism Scientific Studies 8
S Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation Diet 134
md_a Dietary Calcium and Dairy Products Modulate Oxidative and Inflammatory Stress in Mice and Humans Scientific Studies 0
UG Krishnamurti Doxycycline impairs oxidative metabolism and causes eczema and bad skin Supplements, Pharmaceutical Drugs 22
MyUsernameHere Role of oxidative stress / ROS in androgenic alopecia - main mechanism? Hair & Nails 1
R Echinacoside (from cistanche) attenuates ethanol-induced oxidative stress in rat model Scientific Studies 0
Broken man Taurine prevents tamoxifen-induced mitochondrial oxidative damage in mice Scientific Studies 4
haidut Low, Not High, Metabolism Creates Oxidative Stress Scientific Studies 6
Hgreen56 Eat No More That 1 Tsp Fat With A Meal To Prevent Inhibition Of The Oxidative Burning Of Sugar? Fats 1
H Oxidative Respiration On Low Energy Activities Metabolism 12
Grapelander Long-Term L-Serine Administration Reduces Food Intake And Improves Oxidative Stress And Sirt1/NFκB S Scientific Studies 2
Drareg Blood Clots & Strokes In COVID-19; ACE-2 Receptor; Oxidative Stress Viruses, Parasites, Fungus, Vaccines 12
Hans Erectile Dysfunction Part 3: Nitric Oxide, Oxidative Stress And Inflammation Articles & Scientific Studies 0
Lokzo Riboflavin And Pyridoxine Restore Dopamine Levels And Reduce Oxidative Stress In Brain Of Rats Scientific Studies 0
Broken man Diosgenin Protects Against The Oxidative Damaging Effects Of Polyunsaturated Fatty Acids Scientific Studies 1
W Chronic Fatigue Syndrome: Mitchondria, Lactate, Oxidative Stress, Anaerobic Scientific Studies 33
charlie Niacin - Intestinal Permeability And Oxidative Stress In Patients With Alcoholic Pellagra Scientific Studies 6
ecstatichamster Are There Studies Showing How To Inhibit Glycolysis And Encouraging Oxidative Phosphorylation Scientific Studies 14
S Study Claims That Coconut Oil Increase Oxidative Stress Compared To Sesame Oil (High PUFA) Diet 7
A For Those Suffering With Starchs And Oxidative Metabolism In General Ask For Help or Advice 11
RWilly Impact Of Gestational Bisphenol A On Oxidative Stress And Free Fatty Acids Scientific Studies 0
A Chlorine Dioxide And Oxidative Stress Supplements 7
A What Does Oxidative Metabolism Feel Like? Ask For Help or Advice 1
haidut Inosine Powerfully Stimulates Mitochondriogenesis, Oxidative Metabolism & Cell Differentiation Scientific Studies 18
L Minocycline Treatment Increases Resistance To Oxidative Stress And Extends Lifespan In Drosophila Vi Scientific Studies 1
haidut Palmitic Acid (palmitate) Strongly Increases Oxidative Metabolism Scientific Studies 37
J Cannabidiol Protects Neurons From Oxidative Stress Scientific Studies 0
M High Levels Of Oxidative Stress Correlates With Early Onset MPB Health 0
Drareg The Emerging Nexus Of Active DNA Demethylation And Mitochondrial Oxidative Metabolism In Post-Mitoti Scientific Studies 0
Drareg Vitamin K Prevents Oxidative Cell Death By Inhibiting Activation Of 12-lipoxygenase In Developing Ol Scientific Studies 0
Drareg Allopregnanolone Attenuates Aβ25-35-induced Neurotoxicity In PC12 Cells By Reducing Oxidative Stress Scientific Studies 4
Drareg Oxidative Stress In NPC1 Deficient Cells: Protective Effect Of Allopregnanolone Scientific Studies 0
Drareg Effect Of Dihydrotestosterone On Mouse Embryonic Stem Cells Exposed To H2O2-induced Oxidative Stress Scientific Studies 0

Similar threads