Travis
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The endogenous virus theory sounded crazy to me at first. The early videos of Stefan Lanka seemed rational, but when he started talking about viruses as natural cellular messengers I was skeptical.
But this has actually been proven in the lab. It happens. Respectable scientists have induced viruses with radiation and chemicals which are identical to established ones in every respect. They have even been shown to replicate.
Here are some quotes from the article called "Induction of Avian Tumor Viruses in Normal Cells by Physical and
Chemical Carcinogens" published in the journal Virology in 1971.
Weiss, Robin A., et al. "Induction of avian tumor viruses in normal cells by physical and chemical carcinogens." Virology 46.3 (1971): 920-938.
But this has actually been proven in the lab. It happens. Respectable scientists have induced viruses with radiation and chemicals which are identical to established ones in every respect. They have even been shown to replicate.
Here are some quotes from the article called "Induction of Avian Tumor Viruses in Normal Cells by Physical and
Chemical Carcinogens" published in the journal Virology in 1971.
They were shown to be released by by both radiation and 20-methylcholanthrene.The incidence of induction in gs⁻ cells (10 out of 62) was less than that in gs⁺ cells (15 out of 42), though this difference is of doubtful significance. If it is assumed that induction of virus release occurs as an independent event in each irradiated cell...
He did everything: gel electrophoresis of the proteins, gel electrophoresis of the RNA, radioactive nucleotide incorporation assay, antibody binding, and he checked the ability of these induced viruses to be inhibited by other previously-classified viruses (established avian viruses).Biological Characterization of Induced viruses: In the experiments described thus far the induced viruses were identified merely as infectious agents which replicate in pheasant cells and act, like helper viruses for defective RSV [Rous sarcoma virus]. In order to characterize them further, several induced viruses were chosen for studies of their biological and biochemical relationship to known avian tumor viruses.–Weiss
The chemically-induced viruses replicate. These are real viruses.All the ILVs [induced leukosis viruses] proliferated well in gs⁻ pheasant cells, reaching titers of 10⁵to 10⁷ infectious units. –Weiss
The induced viruses (ILVs) band at 1.15, right next to Rous sarcoma virus and HIV.These results indicate that RSV, RAV-0, 7-ILV-1, and I-ILV all behave identically in sucrose density gradient sedimentation. It is concluded that the induced viruses are indistinguishable by this technique from RNA tumor viruses.–Weiss
Baltimore and Temin won a Nobel Prize for this.The DNA polymerase activity first described by Temin and Mizutani (1970) and Baltimore has since been shown to be a property of almost all RNA tumor virions. –Weiss
The chemical and radiation-induced viruses replicated and incorporated tritiated thymidine, an activity that was inhibited by rifampicin. This is a DNA replication inhibitor:...however, additional studies indicated that ILVs respond to added calf thymus DNA, and inhibition with rifampycin derivatives as well approximately 8-fold stimulation of TTP-³H incorporation after the addition of 5μg per reaction of calf thymus DNA. These results indicate that the induced leukosis viruses possess a DNA polymerase activity comparable to that of RAV-0 and other typical RNA tumor viruses. –Weiss
And this wasn't the first time this was done:Rifampicin inhibits bacterial DNA-dependent RNA synthesis by inhibiting bacterial DNA-dependent RNA polymerase. –Wikipedia
They are identical in every respect:Several laboratories have reported the induction of murine RNA tumor viruses following X-irradiation in vivo (Lieberman and Kaplan, 1959, Timmermans et al., 1969), chemical carcinogen in vivo (Ball and McCartcr, 1971) or long-term cultivation in vitro (Hall et al., 1967; Aaronson et al., 1969). However, it has been difficult to characterize the biological properties of these viruses and equally difficult to determine their true origin. Rowe et al. (1971) recently reported convincing evidence of the activation of murine leukemia virus in AKR mouse cells. –Weiss
This article was written in 1971 and he cites Peter Duesberg multiple times. He has four Duesberg articles in the footnotes. Peter Duesberg was certainly a leader in his field, even back then.The characterization of the ILVs reported here amply demonstrates that, these viruses are members of the group known as avian RNA tumor viruses. The virus particles cosediment with known avian tumor viruses and possess the RNA-dependent DNA polymerase, the polypeptides as well as the 70 S RNA typical of avian tumor viruses. They act as helper viruses for RSV by phenotypic mixing. With the exception of C-ILV, they belong to the avian tumor virus subgroup E, as determined by host-range, interference, and antigenicity, although further work is required on variability and possible diversification following extensive propagation of ILV stocks in various cells. –Weiss
Gels contained 2.2% acrylamide in .11% N-N'-methylene bisacrylamide in electrophoresis buffer (Duesberg, 1970). –Weiss
Initiation of virus production is probably a stochastic event whose frequency is greatly enhanced by treatment with ionizing radiations and chemical carcinogens. –Weiss
Weiss, Robin A., et al. "Induction of avian tumor viruses in normal cells by physical and chemical carcinogens." Virology 46.3 (1971): 920-938.
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