Lab Animals Kept Under Stressful Conditions, Thus Affecting Study Results

haidut

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Mar 18, 2013
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17,441
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Several months ago, I did a post on just how stressful the IP administration method is for lab animals. That method is considered the de-facto standard for steroid studies, yet it appears it is so stressful that it can easily produce invalid results due to the its strong effects on the HPA axis and overall hormonal profile. Now, a new study claims that the ambient temperature at which most study rodents are kept is far from optimal and may affect study results, especially ones on cancer and diabetes (which is probably 80%+ of all rodent studies). As an interesting side note, the study noted that mice kept at lower temperatures had faster growing tumors and insulin/glucose dysfunction. This corroborates the ideas that higher metabolism and thus higher body temps are beneficial for health, at least when it comes to cancer. As such, those studies below pour cold water (pun intended) on the claims of benefit from induced "cold thermogenesis" and turning of white fat into brown fat. This process heavily depends on adrenaline and, as one of the study below demonstrates, such stressful phenotype drives both tumor growth and its resistance to therapies. In addition, the process of turning white fat into brown is known to drive cachexia in cancer, likely as a result of elevated lipolysis (which, of course, depends on adrenaline/cortisol).

Baseline tumor growth and immune control in laboratory mice are significantly influenced by subthermoneutral housing temperature - PubMed
https://www.cell.com/pb-assets/journals/trends/cancer/TRECAN59.pdf
UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality - PubMed
Metabolic adaptation of mice in a cool environment - PubMed
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β 2 -adrenergic receptor activation | Nature Communications
Lab mice have a chill, and that may be messing up study results

"...Lab mice are often the first testing ground for human drugs, from vaccines to cancer treatments. But an oft-overlooked detail of their accommodations in laboratories is raising concerns about the results of some mouse studies: the mice have a chill. “It’s still not widely appreciated that the housing temperature that you keep mice at affects the biology and the physiology of the mouse … enough that it can change the [research] outcome,” said Bonnie Hylander, a scientist at the Roswell Park Cancer Institute in Buffalo, N.Y."

"...That was something Hylander realized a few years ago, when the lab in which she was working was studying the impact of temperature on mouse physiology. One of their experiments found that tumors grow more quickly in cold mice than warm mice, and ever since then, Hylander has been looking into the issue."

"...In a study on inflammation, researchers found that mice at cooler temperature had lower insulin and higher glucose levels. Other research has revealed that temperature can impact how plaque builds up in mice’s arteries and can modify how effective some cancer therapies are."
 

LucyL

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Joined
Oct 21, 2013
Messages
709
I've heard Peat say that cold extremities can cause stress hormones to rise. Somebody should look at chronic cold environments of humans - what does setting the thermostat to 68 all winter do? More evidence that global cooling is far more dangerous than global warming.
 

Summer

Member
Joined
Sep 10, 2019
Messages
312
This really reinforces my new habit of dressing warm if I can’t bring up my temps naturally. Yeesh
 

LA

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Joined
Jul 25, 2020
Messages
65
Several months ago, I did a post on just how stressful the IP administration method is for lab animals. That method is considered the de-facto standard for steroid studies, yet it appears it is so stressful that it can easily produce invalid results due to the its strong effects on the HPA axis and overall hormonal profile. Now, a new study claims that the ambient temperature at which most study rodents are kept is far from optimal and may affect study results, especially ones on cancer and diabetes (which is probably 80%+ of all rodent studies). As an interesting side note, the study noted that mice kept at lower temperatures had faster growing tumors and insulin/glucose dysfunction. This corroborates the ideas that higher metabolism and thus higher body temps are beneficial for health, at least when it comes to cancer. As such, those studies below pour cold water (pun intended) on the claims of benefit from induced "cold thermogenesis" and turning of white fat into brown fat. This process heavily depends on adrenaline and, as one of the study below demonstrates, such stressful phenotype drives both tumor growth and its resistance to therapies. In addition, the process of turning white fat into brown is known to drive cachexia in cancer, likely as a result of elevated lipolysis (which, of course, depends on adrenaline/cortisol).

Baseline tumor growth and immune control in laboratory mice are significantly influenced by subthermoneutral housing temperature - PubMed
https://www.cell.com/pb-assets/journals/trends/cancer/TRECAN59.pdf
UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality - PubMed
Metabolic adaptation of mice in a cool environment - PubMed
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β 2 -adrenergic receptor activation | Nature Communications
Lab mice have a chill, and that may be messing up study results

"...Lab mice are often the first testing ground for human drugs, from vaccines to cancer treatments. But an oft-overlooked detail of their accommodations in laboratories is raising concerns about the results of some mouse studies: the mice have a chill. “It’s still not widely appreciated that the housing temperature that you keep mice at affects the biology and the physiology of the mouse … enough that it can change the [research] outcome,” said Bonnie Hylander, a scientist at the Roswell Park Cancer Institute in Buffalo, N.Y."

"...That was something Hylander realized a few years ago, when the lab in which she was working was studying the impact of temperature on mouse physiology. One of their experiments found that tumors grow more quickly in cold mice than warm mice, and ever since then, Hylander has been looking into the issue."

"...In a study on inflammation, researchers found that mice at cooler temperature had lower insulin and higher glucose levels. Other research has revealed that temperature can impact how plaque builds up in mice’s arteries and can modify how effective some cancer therapies are."

Bump
:thumbsup:
 
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