haidut

Member
Joined
Mar 18, 2013
Messages
17,782
Location
USA / Europe
Peat said in one of his interviews that vitamin B2, together with vitamin B1, selenium, vitamin K and CoQ10 are the most important substances for optimal mitochondrial function. I have been researching vitamin B2 and continue to be amazed at how underestimated this nutrient is both in mainstream medicine and in the blogosphere. As many people on the forum know, endotoxin is one of the most important factors involved in suppressing oxidative metabolism and suppressing its production or effects has a host of beneficial effects on overall health and even maximum lifespan. While drugs like methylene blue, mianserin, cyproheptadine, emodin, etc all have their place in the treatment of endotoxemia, vitamin B2 is unique among this group of substances due to its lack of any known side effects. In fact, its safety has earned it a status of food coloring agent that can be used in pretty much any dose the food vendor desires.
These studies below show that vitamin B2, and especially its activated form riboflavin '5 phosphate (R5P), is remarkably effective in inhibiting the systemic effects of endotoxin, including the development of sepsis, as well as other bacterial infections with gram-negative and gram-positive pathogens EVEN after sepsis had already started. In addition, R5P was highly effective in lowering excessive NO production triggered by endotoxin and it seems that adding the amino acid valine greatly enhances the effectiveness of R5P and allows for lower doses to be used to achieve the same effects. Given that riboflavin is a cofactor of the enzyme MAO-A, and that enzyme is inhibited by methylene bue, it looks like the combination of riboflavin and methylene blue can be highly synergistic for issues related to endotoxin and excessive NO synthesis. While the studies did not look into the effects of R5P on serotonin, the role of R5P on MAO-A functionality suggests that some of its beneficial effects on endotoxemia, sepsis and bacterial infections can be at least partly due to its effects of increasing serotonin degradation.
The human equivalent doses were high and given via the IV route. However, oral R5P is very well absorbed and has 90%+ bioavailability, so oral route is also possible. The minimum effective human equivalent oral doses for the combination of R5P and valine were approximately 0.2mg/kg and 25mg/kg respectively. This assumes bioavailability of 90% and 70% for R5P and valine.

Enhancement of resistance to bacterial infection in mice by vitamin B2. - PubMed - NCBI
"...We found that the intramuscular injection of vitamin B2 enhanced host resistance to E. coli infection in a dose-dependent manner (6.25 mg/kg-100 mg/kg). Furthermore, VB2 exhibited the protective activity against Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Actinobacillus pleuropneumoniae. The mechanism of action of VB2 for enhancing resistance in mice may be, at least in part, its ability to stimulate the multiplication of neutrophils and monocytes, and to activate macrophages."

Potentiation by amino acid of the therapeutic effect of highly purified vitamin B2 in mice with lipopolysaccharide-induced shock. - PubMed - NCBI
"...Although aminolevane solution showed no improvement in the lipopolysaccharide-induced mortality, the therapeutic effect of vitamin B2 on endotoxin-induced shock was significantly enhanced by this amino acid supplement. Among the amino acid constituents of aminolevane solution, valine was the most effective in combination therapy of vitamin B2. Vitamin B2 diminished the elevations of interleukin-6 and lactic acid, and ameliorated the increased glucose consumption in lipopolysaccharide-stimulated mouse peritoneal macrophages. The rate of glucose utilization in peritoneal macrophages has been used as an index of lipopolysaccharide-induced activation (Ryan et al., 1979). We speculated that vitamin B2 could play a role in normalizing the energy production of macrophages. Lactic acidosis was reported to increase TNF-a production by rat peritoneal macrophages (Jensen et al., 1990). Further, TNF-a-induced excessive interleukin-6 production is well-known to occur, so lactic acidosis might have induced the excessive produc- tion of interleukin-6 observed in our experiment. The survivors had significantly lower initial lactate levels than nonsurvivors, and were able to significantly decrease their lactate levels over the course of their treatment (Morgan and Machiedo, 2002)."

Highly purified vitamin B2 presents a promising therapeutic strategy for sepsis and septic shock. - PubMed - NCBI
"...Vitamin B2 was administered with a bolus injection 6 h after LPS injection. The survival rate of the control group was 10% (2 of 20). At doses of vitamin B2 of 2.5, 5, 10, and 20 mg/kg of body weight, survival levels were 35% (7 of 20), 65% (13 of 20 [P < 0.05]), 90% (18 of 20 [P < 0.05]), and 95% (19 of 20 [P < 0.05]), respectively (Fig. (Fig.1A).1A). Moreover, treatment with vitamin B2 at 20 mg/kg 6 h after LPS injection not only lowered levels of excessive plasma proinflammatory cytokines, including tumor necrosis factor alpha (Fig. (Fig.2A),2A), interleukin-1 beta (IL-1β) (Fig. (Fig.2B),2B), IL-6 (Fig. (Fig.2C),2C), gamma interferon (Fig. (Fig.2D),2D), monocyte chemotactic protein 1 (Fig. (Fig.2E),2E), and macrophage inflammatory protein 2 (Fig. (Fig.2F),2F), but also decreased NO levels (Fig. (Fig.2G2G)"

"...At 6 h after injection of SEB-d-galactosamine, vitamin B2 was administered with a bolus injection. The survival rate of the control group was 0% (0 of 30). The survival rates of the groups treated with vitamin B2 at 2.5, 5, 10, and 20 mg/kg were 3% (1 of 30), 10% (3 of 30), 27% (8 of 30 [P < 0.05]), and 43% (13 of 30 [P < 0.05]), respectively (Fig. (Fig.1B1B). Vitamin B2 was given 24 h before E. coli inoculation (2.07 × 108 CFU). The survival rate of the control group was 8% (2 of 25). The survival rates of the groups treated with vitamin B2 at 2.5, 5, 10, and 20 mg/kg were 20% (5 of 25), 60% (15 of 25 [P < 0.05]), 76% (19 of 25 [P < 0.05]), and 88% (22 of 25 [P < 0.05]), respectively (Fig. (Fig.1C).1C). In addition, vitamin B2 administered at 20 mg/kg 24 h before E. coli inoculation significantly reduced levels of the bacteria in the blood (Fig. (Fig.33)"

"...Vitamin B2 was given with a bolus injection immediately and 1 and 2 days after S. aureus inoculation (1.69 × 108 CFU). The survival rate of the control group was 0% (0 of 30). The survival rates of the groups treated with vitamin B2 at 2.5, 5, 10, and 20 mg/kg were 0% (0 of 29), 30% (9 of 30 [P < 0.05]), 53% (16 of 30 [P < 0.05]), and 50% (15 of 30 [P < 0.05]), respectively (Fig. (Fig.1D1D). Furthermore, we investigated the therapeutic effect of vitamin B2 in long-term intravenous treatment by infusion using an infusion pump (Natsume, Tokyo, Japan). Infusion of vitamin B2 for 6 h (E. coli infection) or 12 h (S. aureus infection) was begun 1 h after inoculation with bacteria. In the E. coli infection experiments, the survival rate of the control group was 0% (0 of 30). Survival rates of the groups treated with vitamin B2 at 5, 10, 20, and 40 mg/kg/6 h were 20% (2 of 10), 30% (3 of 10 [P < 0.05]), 55% (11 of 20 [P < 0.05]), and 70% (7 of 10 [P < 0.05]), respectively (Fig. (Fig.4A).4A). In the S. aureus infection experiments, the survival rate of the control group was 0% (0 of 10) and the survival rates of the groups treated with vitamin B2 at 20, 40, and 80 mg/kg/12 h were 20% (2 of 10), 50% (5 of 10 [P < 0.05]), and 70% (7 of 10 [P < 0.05]), respectively (Fig. (Fig.4B4B)."

"...Infusion of vitamin B2 with or without APC for 6 h was commenced 6 h after LPS or SEB injection. The survival rate of the control group was 0% (0 of 10) for LPS-treated mice. The survival rates of the groups treated with vitamin B2 (10 mg/kg/6 h), APC (75 units/kg/6 h), and vitamin B2 plus APC were 50% (5 of 10 [P < 0.05]), 30% (3 of 10), and 90% (9 of 10 [P < 0.05]), respectively (Fig. (Fig.4C).4C). In SEB-treated mice, the survival rates of the control group and the groups treated with vitamin B2 (20 mg/kg/6 h), APC (300 units/kg/6 h), and vitamin B2 plus APC were 0% (0 of 10), 60% (6 of 10 [P < 0.05]), 40% (4 of 10), and 90% (9 of 10 [P < 0.05]), respectively (Fig. (Fig.4D4D)."

"...Treatment with vitamin B2 lowered the levels of cytokines (4, 10, 11) and NO (12) that are implicated in the pathogenesis of sepsis and likely enhanced the eradication of bacteria. Thus, vitamin B2 may provide an approach for immunotherapy in the treatment of many inflammatory diseases (including systemic inflammatory response syndrome, cachexia, chronic rheumatism, and nephritis) without excessive suppression of the host defense system. Simultaneous administration of vitamin B2 with APC reduced the mortality of endotoxin- and exotoxin-induced shock, suggesting that therapy with vitamin B2 combined with agents having other properties could be an effective approach to treat sepsis. In conclusion, treatment with highly purified vitamin B2 (riboflavin 5′-sodium phosphate; purity > 97%) reduced levels of mortality in mice with septic shock and may be useful for the treatment of patients with sepsis."
 

Tarmander

Member
Joined
Apr 30, 2015
Messages
3,562
Nice post. I actually was just thinking about this, we must be on the same wavelength. I have had some issues with restless leg, which I attributed to endotoxin. My basic protocol, from reading your previous posts, was 1mg MB, 400mg Riboflavin, and a "crap ton" of coconut oil. Works FANTASTICALLY.

I have had trouble sourcing R5P by itself. I found it in capsules, but the R5P is basically in such a small amount, you are taking a bunch of fillers (10mg R5P, 990mg cellulose bollix). Despite this, I tried the capsule version (Douglas Labs I believe), but I got a sore throat, and bloating which I associate with endotoxin. I switched to a plain Riboflavin powder and these issues went away. So what I really need is a powder version of R5P.
 

milk_lover

Member
Joined
Aug 15, 2015
Messages
1,773
I have a role for myself.. taking the small B vitamins (B1,B2,B3) daily and taking the large B vitamins (B6, B7) biweekly. This has worked best for me. God I get excited when I see a new post by haidut, I always learn new stuff. Thanks for all your effort, man!
 

DaveFoster

Member
Joined
Jul 23, 2015
Messages
4,920
Location
Spokane, Washington
I have a role for myself.. taking the small B vitamins (B1,B2,B3) daily and taking the large B vitamins (B6, B7) biweekly. This has worked best for me. God I get excited when I see a new post by haidut, I always learn new stuff. Thanks for all your effort, man!
Why not B7 daily as well?
 

Elephanto

Member
Joined
May 21, 2015
Messages
820
B2 is one of the only vitamin consistently correlated with prostate cancer. I find it interesting that it's a coenzyme for Mao-a since I already posted a study showing mao-a to promote prostate cancer growth, but I didn't know about the link with B2.

One-carbon metabolism and prostate cancer risk: prospective investigation of seven circulating B vitamins and metabolites. - PubMed - NCBI
Choline and B2 are significantly associated with prostate cancer

Riboflavin at high doses enhances lung cancer cell proliferation, invasion, and migration. - PubMed - NCBI
High dose B2 promotes lung cancer
 

jyb

Member
Joined
Nov 9, 2012
Messages
2,776
Location
UK
Anyone actually tried B2? I never really noticed much from supplementing the B vits (except possibly B3), but I haven't tried them all.
 

lindsay

Member
Joined
Jul 1, 2013
Messages
973
Location
United States
I bought a B2 supplement lately. It looks like Turmeric powder. I have heard from a friend, however, that she tried supplementing B2 last summer because she heard it helps with deterring mosquitos. It gave her a terrible migraine and nausea. So I think taking small doses is super important. Didn't RP say something in one of his interviews like 10 to 15 mg per day for someone who is deficient?
 

800mRepeats

Member
Joined
Jul 13, 2015
Messages
158
Nice post. I actually was just thinking about this, we must be on the same wavelength. I have had some issues with restless leg, which I attributed to endotoxin. My basic protocol, from reading your previous posts, was 1mg MB, 400mg Riboflavin, and a "crap ton" of coconut oil. Works FANTASTICALLY.

I have had trouble sourcing R5P by itself. I found it in capsules, but the R5P is basically in such a small amount, you are taking a bunch of fillers (10mg R5P, 990mg cellulose bollix). Despite this, I tried the capsule version (Douglas Labs I believe), but I got a sore throat, and bloating which I associate with endotoxin. I switched to a plain Riboflavin powder and these issues went away. So what I really need is a powder version of R5P.

Is Pure Bulk an option for you?
Riboflavin 5 Phosphate - Pure Bulk Supplements
 

lindsay

Member
Joined
Jul 1, 2013
Messages
973
Location
United States
Anyone actually tried B2?

I have taken it in small doses and in my espresso (which apparently has a decent amount of Riboflavin and a lot more Niacin than regular coffee - probably one of the reasons it's good for the liver). I haven't really noticed much of a difference, but I'm also not super fond of MB, though I've only used a drop of it when my tummy is upset. Ginger oil is way more powerful IMO. In fact, I was trying to find studies today because I read in quite a few places that ginger inhibits serotonin uptake in the gut (aka, serotonin "receptors"), which is why it's so powerful against nausea. I love the stuff.
 
Last edited:
Joined
Nov 21, 2015
Messages
8,723
Damn Purebulk's R5P is not cheap. Close to $0.50/gram!
But normally you don't need more than 10 milligrams or so. And if u are sick then the cost isn't that important because 50 cents a gram would mean it costs a few bucks to recover instead of costly and potentially harmful antibiotics
 

Giraffe

Member
Joined
Jun 20, 2015
Messages
3,375
B2 is one of the only vitamin consistently correlated with prostate cancer. I find it interesting that it's a coenzyme for Mao-a since I already posted a study showing mao-a to promote prostate cancer growth, but I didn't know about the link with B2.

One-carbon metabolism and prostate cancer risk: prospective investigation of seven circulating B vitamins and metabolites. - PubMed - NCBI
Choline and B2 are significantly associated with prostate cancer

Riboflavin at high doses enhances lung cancer cell proliferation, invasion, and migration. - PubMed - NCBI
High dose B2 promotes lung cancer
The cell culture study found that "Riboflavin at 200 and 400 μmol/L significantly enhanced cell growth in test lung cancer cell lines." This is 5,000 times the highest concentration they found is the blood plasma in that observational human study.

Regarding that observational study, it is very mysterious how they came to their conclusions. I couldn't find a single hint.
 

robknob

Member
Joined
Feb 1, 2016
Messages
384
Location
St Louis
Cronometer has egg whites at about 1mg riboflavin per cup, and with dairy I can get about 6-7 mg from food a day. The b2 supplement I have doesn't have any noticeable effect on me, and it seems to pass through my urine within about 18 hours. I don't think it gets absorbed at all...
 

jaa

Member
Joined
Dec 1, 2012
Messages
1,035
But normally you don't need more than 10 milligrams or so. And if u are sick then the cost isn't that important because 50 cents a gram would mean it costs a few bucks to recover instead of costly and potentially harmful antibiotics

True. My internal calculator wasn't working well last night :D.
 

milk_lover

Member
Joined
Aug 15, 2015
Messages
1,773
Why not B7 daily as well?
I tend to take my B vitamins with lunch that barely has enough sugar (coke or two with a lot of meat, which might not be that healthy) and If I take huge amounts of biotin (5 mg) I get hypoglycemia symptoms. It's very powerful in reducing blood sugar. When I had the 300 mcg pills, I was taking biotin almost everyday.
 

haidut

Member
Thread starter
Joined
Mar 18, 2013
Messages
17,782
Location
USA / Europe
Thank you for this great post. B vits are dear to your heart, aren't they?

Yep, I think for people without access to thyroid supplement the B vitamins is one of the few safe alternatives. Given the recent blockbuster performance of biotin in human trials of MS it would not be long before FDA requires a prescription for all of them.
 
Similar threads
Thread starter Title Forum Replies Date
haidut Vitamin B3 Remarkably Therapeutic For Brain Cancer (glioblastoma) Scientific Studies 2
G What vitamin deficiencies lead to acne? Ask For Help or Advice 4
haidut Vitamin D improves ovarian reserves in women with infertility Scientific Studies 1
haidut Vitamin E 100-fold more potent than remdesivir for SARS-CoV-2 / coronaviruses Scientific Studies 52
F Pasteurized squeezed orange juice mineral/vitamin content? Ask For Help or Advice 1
G How do animals make vitamin B12 Vitamins 1
Lenny Vitamin K and aspirin, how to proceed? Ask For Help or Advice 8
C Do you still get vitamin D on a fully cloudy sky? D 17
M Help me reduce my vitamin D levels Ask For Help or Advice 21
S Vitamin E dose for lowering estrogen and prolactin? Supplements 14
youngsinatra Vitamin A + Iron Supplementation As Effective As TRT In Puberty-Delayed Individuals Male Issues 1
md_a Vitamin D, Calcium and mineral metabolism with Dr Ray Peat and Kate Deering Interviews 6
haidut High PTH, low vitamin D implicated in bipolar disorder (BD) Scientific Studies 2
haidut Vitamin B6, lowering stress, may treat schizophrenia Scientific Studies 3
haidut Vitamin D (topical) safe and effective for removing scars Scientific Studies 2
haidut Vitamin D deficiency impairs muscles by lowering energy production Scientific Studies 2
Pistachio Vitamin D3 perpetual thread (for-and-against testmonials) Supplements 41
S Feeling worse with sun instead of vitamin D supp? Ask For Help or Advice 42
md_a High Levels of Active 1,25-Dihydroxyvitamin D Despite Low Levels of the 25-Hydroxyvitamin D Precursor - Implications of Dysregulated Vitamin D for Dia Articles & Scientific Studies 0
Pina Vitamin K1 (phylloquinone) induces vascular endothelial dysfunction Vitamins 0
L Vitamin D from sun making me feel worse? Ask For Help or Advice 13
M Do ice cream, cheese, yogurt , cream and other dairy products come from vitamin fortified milk? Dairy 41
J How many drops of Vitamin D oil should you be applying to your skin per day MAXIMUM? Vitamins 6
livesimply Amazing article: Vitamin C and Coronavirus: Not a Vaccine, Just a Humble Cure Viruses, Parasites, Fungus, Vaccines 12
theotokos Cold sores after taking vitamin E? Supplements, Pharmaceutical Drugs 1
yerrag Is There Something Wrong About How We Get Vitamin D From Sunlight? I Suspect We Do It In a Very Unnatural Way Vitamins 43
J Feeling incredibly Dried-Out after breakfast (includes liver): Vitamin A Toxicity? Diet 22
T Vitamin K2 5 mk4 5 mg ruined my face Ask For Help or Advice 9
T Thorne Research vitamin K2 liquid topically does nothing to me Ask For Help or Advice 6
xeliex You have to test your vitamin D! D 7
md_a Chronic vitamin D deficiency induces lung fibrosis through activation of the renin-angiotensin system Scientific Studies 0
T Vitamin D heals scar tissue...possible treatment for the dreaded Peyronies Disease? Ask For Help or Advice 6
T Does coffee interfere with vitamin E oral absorption? Ask For Help or Advice 2
CHEESEHEAD69 Is it effective to pop open a Vitamin D softgel capsule and apply the d3 oil that was inside topically? Ask For Help or Advice 4
D Podcast with Jim Stephenson Jr. on vitamin/hormone D Supplements, Pharmaceutical Drugs 1
T Experiences with Jarrow Formulas vitamin D3 2500 IU? Ask For Help or Advice 0
GorillaHead Vitamin B5 supplementation may cause adrenal over activity? Ask For Help or Advice 2
Doludolu Very LOW Iron , Sufficient Ferritin, Excesivelly high vitamin B12 - Anemia or what is going on? Blood Work, Labs 9
T Now Foods Vitamin E 400 mixed tocopherols experiences and reviews Ask For Help or Advice 6
T Will Thorne research liquid vitamin K2 absorb through the skin? Ask For Help or Advice 8
bzmazu More vitamin K2, more testosterone K 1
ddjd High vitamin D levels essential for tolerating T3?? Ask For Help or Advice 0
L Best vitamin c for a child? Ask For Help or Advice 6
Mito The Great Vitamin D Debate with Georgi Dinkov, Danny Roddy, Kate Deering & Matt Blackburn D 41
M Does a lot of copper increase the need of vitamin C? Ask For Help or Advice 10
Mauritio Vitamin B1 / Thiamine deficiency increases serotonin in the brain Scientific Studies 32
P Study: Vitamin A theory of vaccine injury Scientific Studies 26
haidut Vitamin D can force cancer cells to differentiate back into normal Scientific Studies 1
haidut Oral testosterone + vitamin E may treat NAFLD, NASH, cirrhosis in humans Scientific Studies 6
haidut Glucose + vitamin B3 combo synergistic for treating mitochondrial disease Scientific Studies 1

Similar threads

Top