Sefton10
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- Oct 19, 2019
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Excellent information, thank you for such a thorough break down!Oh yeah. Cool. Thanks.
thymoquinone in seeds (in mg)
0.0098 - 0.376 % of weight
I found this volume to weight conversion for black cumin seeds:
AMAZING HERBS, BLACK CUMIN SEED,...28525 volume to weight conversion
Calculate weight of AMAZING HERBS, BLACK CUMIN SEED, UPC: 665231862164 per volume, it weighs 254 g/metric cup (8.5 oz/US cup).www.aqua-calc.com
The volume measurement can be changed to tablespoon.
So the weight of one tablespoon of black cumin seeds is 15 grams or 0.53 ounce.
Thymoquinone is 0.0098 - 0.376 % of weight.
15 g x 0.000098 = 0.00147 g = 1.47 mg
15 g x 0.00376 = 0.0564 g = 56.4 mg
So, because that's a big range, I'm gonna look at what the data points excluding the extremes are, and get an average calculated without those extremes.
(page 5)
View attachment 24818
So after excluding the maximum (0.376%) and minimum (0.010%), then there are pretty much two groups: around 0.100% and around 0.015% percent.
So the average of the ~0.100% group: (0.101 + 0.107 + 0.085 + 0.107 + 0.167) / 5
= 0.1134%
The average of the ~0.015% group:
(0.015 + 0.021 + 0.014) / 3
= 0.0167%
So amount of thymoquinone in a tablespoon (15 g) of seeds using those percentages...
15 g x 0.00134 = 0.0201 g = 20 mg
15 g x 0.000167 = 0.0025 g = 2.5 mg
2.5 - 20 mg.
:\
I guess hope you get seeds that are about 0.1% thymoquinone instead of 0.01%.
So assuming you get the 0.01% TQ seeds, you could eat 10 tablespoons and assume you're getting as much TQ as eating 1 tablespoon of the 0.1% TQ seeds. But if that's a wrong assumption and you're eating the 0.1% TQ seeds, then you'd be eating ~200 mg of TQ. You could split your bets and use five tablespoons, and so be eating either ~10-15 mg or ~100 mg.
_____
Next, the dosages of thymoquinone in the oil...
View attachment 24819
Removing the minimum and maximum makes the range 0.142% - 0.486%.
I got an 8 oz bottle of black cumin seed oil, and it says on the label serving size of 1 teaspoon (5 ml) has 4.6 grams weight of oil in it. Compare that to the seeds weighing 15 grams per tablespoon. So to compare the dosages of seeds and oil I'll calculate the amount of thymoquinone in 3 of the teaspoon servings (3 tsp=1 tbsp), 14.2 grams, of the oil.
14.2 g x .00142 = .0201 g = 20 mg
14.2 g x .00486 = .069 g = 69 mg
So 20 - 69 mg thymoquinone per 3 teaspoons (1 tablespoon) of the oil.
Compared to 2.5 - 20 mg in 1 tablespoon (and about the same weight) of the seeds.
__________
So how much is safe?
The Effect of Route of Administration in Thymoquinone Toxicity in Male and Female Rats
The maximum tolerated dose for intraperitoneal injection and oral ingestion of thymoquinone was determined in male and female Wistar rats. A range of dose levels of thymoquinone: 20, 30 and 40 mg/kg body weight for intraperitoneal injection and 200, 300 ...www.ncbi.nlm.nih.gov
"The maximum tolerated dose for intraperitoneal injection and oral ingestion of thymoquinone was determined in male and female Wistar rats. A range of dose levels of thymoquinone: ... 200, 300 and 500 mg/kg body weight for oral ingestion... tested for acute toxicity in rats... The results showed that the maximum tolerated dose for... oral ingestion was 250 mg/kg in both male and female rats. There were different signs of toxicity shown in rats which received intraperitoneal injection from those that received oral ingestion of thymoquinone... Rats which received oral ingestion of thymoquinone showed transient toxicity signs. Two deaths were reported at dose of 500 mg/kg as a result of bowel obstruction complications."
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"Several toxicological studies indicated that oral administration of TQ in the range of 10 to 100 mg/kg has no toxic or lethal effects in mice.45-49"
Therapeutic Implications of Black Seed and Its Constituent Thymoquinone in the Prevention of Cancer through Inactivation and Activation of Molecular Pathways
The cancer is probably the most dreaded disease in both men and women and also major health problem worldwide. Despite its high prevalence, the exact molecular mechanisms of the development and progression are not fully understood. The current chemotherapy/radiotherapy regime used to treat...www.hindawi.com
"The oral and intraperitoneal lethal dose (LD50) in animal models such as rats and mice has been successfully assigned by earlier researcher [143, 144]. The safety, tolerability, and less toxicity of N. sativa at higher doses are established via human clinical trials. A study showed that TQ at the concentrations of 0 to 10μM was not cytotoxic to fibroblast-like synoviocytes [145]. Earlier study showed that Nigella sativa did not show any toxicity effect on liver and supplementations of Nigella sativa reduce the alanine aminotransferase (ALT) level and aspartate aminotransferase (AST) level treated rats compared to the control doses of rats [146]. Another study reported that 0.6 mg/kg/day oral dose of TQ is suitable for humans [145] and 0.05 mg/kg/day of Nigella sativa extract for postmenopausal women [147]. Another report showed that the LD50 of oral administration of TQ was 2.4 gm/kg (1.52–3.77, 95% CL). Numerous studies in the support of N. sativa have shown that no evidence of N. sativa fixed oil toxicity was observed, when administered in different doses up to 10 mL/kg body wt., p.o. [148]. N. sativa and its seed powder did not show any toxic effects and were safe at very high doses (28 gm/kg orally) and its oil was also safe at dose (28.8 ml/kg) in rabbits and rats respectively [149, 150]."
"Another study reported that 0.6 mg/kg/day oral dose of TQ is suitable for humans"
200 lb person = 90.7 kg
0.6 mg/kg x 90.7 kg = 54.4 mg per day safe dose for humans (apparently?)
The range of thymoquinone in 1 tablespoon of oil is 20 - 69 mg. The range in 1 tablespoon of seeds is 2.5 - 20 mg.
I'll try to find info about dosages of thymoquinone that are effective for neuroprotection next.
It seems the oil is the standout if thymoquinone is the aim. I have a bottle that is supposed to be 3.7%, which is higher than standard oils I think. The downside of the oil is obviously the PUFA content if going overboard, but a tsp a day seems it would be worth the trade off.
This Twitter thread contains a lot of studies on the benefits of black cumin seeds.
View: https://twitter.com/grimhood/status/1382729395987615745?s=21