zarrin77
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Sodium bicarbonate increases glucose uptake and mitochondrial biogenesis in C2C12 myotubes potentially via the transcriptional co-activator PGC-1α - The Physiological Society
Bicarbonate is one of the most effective sports supplements for athletes out right now, and works better than beta-leaning for pH regulation and performance.
Soluble adenylyl cyclase (sAC) is a *cytoplasmic* enzyme that is activated by bicarbonate to increase cAMP production. This is a novel way to stimulate cAMP outside of the froskolin-induced mechanism affecting the “transmembrane” AC. (BTW, caffeine is a PDE inhibitor, which stops the breakdown of cAMP, possible synergism?)
Anyway....
“NaHCO3 (sodium bicarbonate) treatment increased the expression of PGC-1α (302%), GLUT4 (266%) and cytochrome-c (291%) whilst increasing the protein content of GLUT4 (37%), MCT1 and the respiratory chain subunits COX-2 (63%) and COX-4 (42%). Both basal and insulin stimulated glucose uptake were increased following NaHCO3 treatment (68% and 73% respectively) as was basal cell oxygen consumption (10%). Finally NaHCO3 increased the abundance of membrane bound GLUT4 (68%) and MCT1 (73%). Taken together, these findings suggest that NaHCO3 induced chronic alkalosis promotes up-regulation of PGC-1α and its downstream targets COX-2, COX-4, Cytochrome-c and MCT1. Further, NaHCO3 also increases basal and insulin stimulated glucose uptake via increased GLUT4 expression, content and membrane localisation.(sic)”
This study helps show the molecular mechanism by which bicarbonate helps not only improve performance, but also muscle adaptations from exercise.
This is specific to bicarbonate (or CO2) most likely, as sAC is activated specifically by bicarbonate, not pH.
More references:
- pH sensing via bicarbonate-regulated “soluble” adenylyl cyclase (sAC)
- Role of the bicarbonate-responsive soluble adenylyl cyclase in pH sensing and metabolic regulation
- Sodium bicarbonate ingestion augments the increase in PGC-1α mRNA expression during recovery from intense interval exercise in human skeletal muscle
- The PGC-1α-related Coactivator Promotes Mitochondrial and Myogenic Adaptations in C2C12 Myotubes - PubMed
Bicarbonate is one of the most effective sports supplements for athletes out right now, and works better than beta-leaning for pH regulation and performance.
Soluble adenylyl cyclase (sAC) is a *cytoplasmic* enzyme that is activated by bicarbonate to increase cAMP production. This is a novel way to stimulate cAMP outside of the froskolin-induced mechanism affecting the “transmembrane” AC. (BTW, caffeine is a PDE inhibitor, which stops the breakdown of cAMP, possible synergism?)
Anyway....
“NaHCO3 (sodium bicarbonate) treatment increased the expression of PGC-1α (302%), GLUT4 (266%) and cytochrome-c (291%) whilst increasing the protein content of GLUT4 (37%), MCT1 and the respiratory chain subunits COX-2 (63%) and COX-4 (42%). Both basal and insulin stimulated glucose uptake were increased following NaHCO3 treatment (68% and 73% respectively) as was basal cell oxygen consumption (10%). Finally NaHCO3 increased the abundance of membrane bound GLUT4 (68%) and MCT1 (73%). Taken together, these findings suggest that NaHCO3 induced chronic alkalosis promotes up-regulation of PGC-1α and its downstream targets COX-2, COX-4, Cytochrome-c and MCT1. Further, NaHCO3 also increases basal and insulin stimulated glucose uptake via increased GLUT4 expression, content and membrane localisation.(sic)”
This study helps show the molecular mechanism by which bicarbonate helps not only improve performance, but also muscle adaptations from exercise.
This is specific to bicarbonate (or CO2) most likely, as sAC is activated specifically by bicarbonate, not pH.
More references:
- pH sensing via bicarbonate-regulated “soluble” adenylyl cyclase (sAC)
- Role of the bicarbonate-responsive soluble adenylyl cyclase in pH sensing and metabolic regulation
- Sodium bicarbonate ingestion augments the increase in PGC-1α mRNA expression during recovery from intense interval exercise in human skeletal muscle
- The PGC-1α-related Coactivator Promotes Mitochondrial and Myogenic Adaptations in C2C12 Myotubes - PubMed
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