Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host

[area51puy]

Another important signalling molecule implicated in host–microbe crosstalk is serotonin, an important human neurotransmitter. Serotonin is sensed by P. aeruginosa evidenced by Knecht and colleagues (2016) who have shown its ability to act in regulating P. aeruginosa virulence in a mouse model of infection. Here, an observed upregulation of biofilm formation and virulence factor production is described (Knecht et al. 2016). Interestingly, the endogenous opioid dynorphin—a κ-agonist and mediator of pain suppression—has been implicated in an enhancement of P. aeruginosa’s virulence through its interaction with one of the QS systems, PQS (Valentino and Van Bockstaele 2015). Histamine, another neurotransmitter which also plays an important role in allergic reactions is elevated in mice infected with P. aeruginosa through neutrophil-mediated histamine production, reflecting multiple potential crossovers between microbial endocrinology and host immune responses

 

[area51puy]

Influence of acetylsalicylic acid (aspirin) on biofilm production by Candida species - PubMed

Candida spp. are important causative agents of infections associated with biofilm formation. Management of biofilm-related infections is extremely difficult and therefore new therapeutic solutions are needed. This study for the first time explored the possible effect of aspirin on Candida spp...

pubmed.ncbi.nlm.nih.gov

 

[area51puy]

Acetoacetate and ethyl acetoacetate as novel inhibitors of bacterial biofilm - PubMed

Bacterial biofilms are communities of bacteria that form on surfaces and are extremely difficult to remove by conventional physical or chemical techniques, antibiotics or the human immune system. Despite advanced technologies, biofilm still contributes to 60 to 80% of human bacterial infections...

pubmed.ncbi.nlm.nih.gov

 

[area51puy]

Ethyl Pyruvate: An Anti-Microbial Agent that Selectively Targets Pathobionts and Biofilms - PubMed

The microbiota has a strong influence on health and disease in humans. A causative shift favoring pathobionts is strongly linked to diseases. Therefore, anti-microbial agents selectively targeting potential pathogens as well as their biofilms are urgently demanded. Here we demonstrate the impact...

pubmed.ncbi.nlm.nih.gov

 

[area51puy]

Vitamin E for Prevention of Biofilm-caused Healthcare-associated Infections

The healthcare-associated infections (HCAIs) occur in patients both in nosocomial environments and in community. More often HCAIs are associated to the use of medical devices and bacterial biofilm development on these equipments. Due to the clinical and ...

www.ncbi.nlm.nih.gov

 

[area51puy]

Naringenin as an antibacterial reagent controlling of biofilm formation and fatty acid metabolism in MRSA

MRSA is Methicillin-resistant Staphylococcus aureus and they are widespread and making trouble in treatment in communities and surgical areas. MRSA have been adapted to antibiotics so that they can block the access of antibiotics physically or chemically deactivate it or modify the precursor of...

www.biorxiv.org

 

[area51puy]

Naringin sensitizes the antibiofilm effect of ciprofloxacin and tetracycline against Pseudomonas aeruginosa biofilm

The study aims to explore the combinatorial effect of naringin with antibiotics, ciprofloxacin and tetracycline on Pseudomonas aeruginosa biofilms. Th…

www.sciencedirect.com

 

[area51puy]

The flavanone naringenin reduces the production of quorum sensing-controlled virulence factors in Pseudomonas aeruginosa PAO1

Preliminary screening of the Malagasy plant Combretum albiflorum for compounds attenuating the production of quorum sensing (QS)-controlled virulence factors in bacteria led to the identification of active fractions containing flavonoids. In the present study, several flavonoids belonging to the...

www.microbiologyresearch.org

The flavanone naringenin reduces the production of quorum sensing-controlled virulence factors in Pseudomonas aeruginosa PAO1
Olivier M. Vandeputte,1,23 Martin Kien

 

[area51puy]

New Insight into Vitamins E and K1 as Anti-Quorum-Sensing Agents against Pseudomonas aeruginosa - PubMed

Today, antivirulence compounds that attenuate bacterial pathogenicity and have no interference with bacterial viability or growth are introduced as the next generation of antibacterial agents. However, the development of such compounds that can be used by humans is restricted by various factors...

pubmed.ncbi.nlm.nih.gov

vitamin E and K1, as anti-quorum sensing agents against Pseudomonas aeruginosa; one of the hazardous pathogens to human life responsible for several diseases. Both vitamins showed significant anti-biofilm activity (62% and 40.3% reduction by vitamin E and K1, respectively) and the expression of virulence factors including pyocyanin, pyoverdine, and protease was significantly inhibited, especially in the presence of vitamin E. Co-treatment of constructed biofilms with these vitamins plus tobramycin significantly reduced the number of bacterial cells sheltered inside the impermeable matrix (71.6% and 69% by a combination of tobramycin and vitamin E or K1, respectively). The in silico studies besides the similarities of chemical structures, reinforce the possibility that both vitamins act through inhibition of the PqsR protein.This is the first report about the anti-virulent and anti-pathogenic activity of vitamin E and K1 against P. aeruginosa and confirms their potential for further research against other multi-drug resistant bacteria.

 

[area51puy]

Quinine Enhances Photo-Inactivation of Gram-Negative Bacteria

AbstractBackground. Antimicrobial resistance is a significant concern to public health, and there is a pressing need to develop novel antimicrobial therape

academic.oup.com

In this study, we investigated the capacity for quinine hydrochloride (Q-HCL) to enhance the antimicrobial effects of antimicrobial blue light ([aBL] 405 nm wavelength) against multidrug-resistant (MDR) Gram-negative bacteria in vitro and in vivo.
Results
Our findings demonstrated the significant improvement in the inactivation of MDR Pseudomonas aeruginosa and Acinetobacter baumannii (planktonic cells and biofilms) when aBL was illuminated during Q-HCL exposure. Furthermore, the addition of Q-HCL significantly potentiated the antimicrobial effects of aBL in a mouse skin abrasion infection model. In addition, combined exposure of aBL and Q-HCL did not result in any significant apoptosis when exposed to uninfected mouse skin.
Conclusions
In conclusion, aBL in combination with Q-HCL may offer a novel approach for the treatment of infections caused by MDR bacteria.

 

[area51puy]

Cortisol enhances the biofilm formation of Streptococcus mutans | Request PDF

Request PDF | Cortisol enhances the biofilm formation of Streptococcus mutans | Cortisol, a hormone produced by adrenal cortex and released in response to psychological stress, is found in different body fluids, such as serum... | Find, read and cite all the research you need on ResearchGate

www.researchgate.net

 

[area51puy]

Steroid hormones as interkingdom signaling molecules: Innate immune function and microbial colonization modulation

Steroid hormones e.g., estrogen, progesterone, testosterone and dehydroepiandosterone, act as inter-kingdom quorum chemical signaling compounds. All steroids examined exhibit a steroid concentration specific bi-functionality. At one end of the spectrum, the steroids enhance expression of...

www.aimspress.com

Steroid hormones e.g., estrogen, progesterone, testosterone and dehydroepiandosterone, act as inter-kingdom quorum chemical signaling compounds. All steroids examined exhibit a steroid concentration specific bi-functionality. At one end of the spectrum, the steroids enhance expression of virulence-associated behaviors, most specifically, increased rate of replication and adherence to surfaces. In contrast, the hormones also function as innate immune system compounds providing first-line protection against essential pathogen behaviors e.g., biofilm formation, which plays a role in initiation of the vast majority of infectious processes, especially chronic infections. Mechanistically, this protection is mediated by both direct effects of steroids on microbes, as well as indirect actions which result in expression of nitric oxide at levels reported to inhibit proper biofilm formation and cause return of sessile cells to a planktonic state.

 

[supercoolguy]

What The Fook!?

 

[area51puy]

 

[area51puy]

all the studies I posted were mostly of stuff recommended from Ray Peat or Haidut and how they inhibit biofilms and some stuff like how cortisol ,serotonin and estrogen can proliferate the growth of biofilms.

so some of the benefits of the stuff they recommended maybe can be contributed to the breakdown and inhibition of biofilms so antibiotics and your immune system can get rid of bacteria that lead to endotoxins and other health problems like ibs and crohns and some autoimmune diseases.

I think biofilms in intestines, mouth and nose are not seen as the problem they are. And they are contributing to many health problems.

biofilms are stubborn and that is what allows bacteria avoid our immune system antibiotics.

 

[area51puy]

While single bacteria may be susceptible to antibiotics, the films can be 1,000 times more resistant and most can only be removed surgically.

 

[Broken man]

@area51puy you are beast!!

 

[Broken man]

Influence of acetylsalicylic acid (aspirin) on biofilm production by Candida species - PubMed

Candida spp. are important causative agents of infections associated with biofilm formation. Management of biofilm-related infections is extremely difficult and therefore new therapeutic solutions are needed. This study for the first time explored the possible effect of aspirin on Candida spp...

pubmed.ncbi.nlm.nih.gov

For aspirin to reach candida, I think phenol salicylate Is needed.

 

[area51puy]

Artemisia princeps Inhibits Biofilm Formation and Virulence-Factor Expression of Antibiotic-Resistant Bacteria - PubMed

<p>In this study, we used ethanol extract of A. princeps and investigated its antibacterial effects against MRSA. Ethanol extract of A. princeps significantly inhibited MRSA growth and organic acid production during glucose metabolism at concentrations greater than 1 mg/mL (P < 0.05). MRSA...

pubmed.ncbi.nlm.nih.gov

 

[area51puy]

Inhibition of streptococcal biofilm by hydrogen water

Abstract Objectives The accumulation of oral bacterial biofilm is the main etiological factor of oral diseases. Recently, electrolyzed hydrogen-rich water (H-water) has been shown to act as an effe…

pocketdentistry.com