Role Of G6PDH Inhibition In The Antiproliferative Effects Of DHEA

[Drareg]

Danny Roddy wrote an interesting article on G6PDH, it's a crucial enzyme it seems, mainstream were saying DHEA inhibits G6PDH but this study implies at low doses it does not have this effect, it seems researches have been behaving the same way with DHEA in studies using high doses so it could be estrogen?
It's does dependent going by the studies below.

@haidut what is 10microM in human terms ? Peat recommended amount I'm guessing.


Role of glucose-6-phosphate dehydrogenase inhibition in the antiproliferative effects of dehydroepiandrosterone on human breast cancer cells.

Epidemiological and experimental studies suggest that dehydroepiandrosterone (DHEA) exerts a protective effect against breast cancer. It has been proposed that the non-competitive inhibition of glucose-6-phosphate dehydrogenase (G6PD) contributes to DHEA antitumor action. We evaluated the effects of DHEA on G6PD activity and on the in vitro proliferation of two human breast cancer cell lines, MCF-7 (steroid receptor positive) and MDA-MB-231 (steroid receptor negative), in a serum-free assay. tDHEA inhibition of G6PD was only found to occur at concentrations above 10 microM; at these high concentrations, the growth curve was parallel to the enzyme inhibition curve in both cell lines. In contrast, at concentrations in the in vivo breast tissue concentration range, neither cell growth nor enzyme activity was inhibited. The results failed to confirm DHEA's putative anti-tumor action on breast cancer through G6PD inhibition, as the enzyme blockade only becomes apparent at pharmacological concentrations of the steroid

In the following study DHT is shown to increase G6PDH, I can't copy and paste for some reason, if you scroll down you will see the insights they get to DHT effects on G6PDH in human sperm.

Human sperm anatomy and endocrinology in varicocele: Role of androgen receptor

 

[haidut]

Danny Roddy wrote an interesting article on G6PDH, it's a crucial enzyme it seems, mainstream were saying DHEA inhibits G6PDH but this study implies at low doses it does not have this effect, it seems researches have been behaving the same way with DHEA in studies using high doses so it could be estrogen?
It's does dependent going by the studies below.

@haidut what is 10microM in human terms ? Peat recommended amount I'm guessing.


Role of glucose-6-phosphate dehydrogenase inhibition in the antiproliferative effects of dehydroepiandrosterone on human breast cancer cells.

Epidemiological and experimental studies suggest that dehydroepiandrosterone (DHEA) exerts a protective effect against breast cancer. It has been proposed that the non-competitive inhibition of glucose-6-phosphate dehydrogenase (G6PD) contributes to DHEA antitumor action. We evaluated the effects of DHEA on G6PD activity and on the in vitro proliferation of two human breast cancer cell lines, MCF-7 (steroid receptor positive) and MDA-MB-231 (steroid receptor negative), in a serum-free assay. tDHEA inhibition of G6PD was only found to occur at concentrations above 10 microM; at these high concentrations, the growth curve was parallel to the enzyme inhibition curve in both cell lines. In contrast, at concentrations in the in vivo breast tissue concentration range, neither cell growth nor enzyme activity was inhibited. The results failed to confirm DHEA's putative anti-tumor action on breast cancer through G6PD inhibition, as the enzyme blockade only becomes apparent at pharmacological concentrations of the steroid

In the following study DHT is shown to increase G6PDH, I can't copy and paste for some reason, if you scroll down you will see the insights they get to DHT effects on G6PDH in human sperm.

Human sperm anatomy and endocrinology in varicocele: Role of androgen receptor

About 5mg DHEA achieves 1microM, so assuming linear scaling this means 50mg DHEA. Given that this is a bit much for a single dose, I think using the 5mg x 3 daily over a week would also achieve the needed concentration much more safely.