Progesterone receptors and serotonin levels in colon epithelial cells from females with slow transit constipation (P4 lowers SERT)


May 2, 2020


link to study

Background: Females with slow transit constipation (STC) exhibit progesterone receptor (P4R) overexpression in colon muscle that impair their contractility. These studies examined whether these patients have an overexpression of P4R in epithelial cells and whether P4 affects the SERT-5-HT pathway.

Methods: Tissues were obtained from surgical specimens of seven females with STC and six controls. Feasibility studies were performed in biopsies from six patients with STC and three controls. P4R, SERT and TPH-1 mRNA and protein expression and 5-HT by ELISA were determined. Contraction was studied in normal muscle cells pretreated with P4 or buffer. Progesterone effects on SERT and 5-HT levels were studied in normal human mucosa in vitro and in wild and SERT knockout mice in vivo.

Key results: P4R was overexpressed in epithelial cells in STC compared with controls. The levels of SERT were lower and 5-HT higher in STC. In epithelial cells P4 treatment decreased SERT and increased mucosal 5-HT without affecting TPH-1. Progesterone impaired the contraction of normal muscle cells induced by Ach and 5-HT. Progesterone decreased SERT and increased 5-HT levels in the colon of wild mice in vivo but had no effect on the high basal levels of 5-HT in SERT knockout mice.

Conclusions & inferences: P4R are present in colon epithelial cells and are overexpressed in females with STC. These cells have reduced SERT and high 5-HT levels and normal TPH-1. These 5-HT signaling abnormalities are related to overexpression of P4R since they are reproduced in human epithelial cells in vitro and in mice in vivo.

P4 raises 5ht and lowers SERT in cells :/
If it does this throughout the body be sure not to take other things that block SERT such as too much vitamin d or of course an ssri, personally I had problems of high serotonin with vitamin d (4000iu) with around 20mg p4 , constipation and worryingly testicular pain as well as a depressed defeated mood.

Melatonin inhibits SERT mediated 5‐HT uptake in human and mouse derived platelets and synaptosomes​

Melatonin also increases the action of progesterone/ is anti estrogenic and in my case taking high doese p4 and melatonin 3mg would block sert action even more so be careful with that combo.


Thread starter
May 2, 2020
P4 is a serotonin reuptake inhibitor it lowers sert in body and brain.


Apr 18, 2018
Progesterone is awesome.

Similar threads
Thread starter Title Forum Replies Date
maillol Progesterone, Progesterone Receptors and Prolactin Scientific Studies 1
K Cross Talk Between Progesterone And Estrogen Receptors In Breast Cancer Articles & Scientific Studies 1
A Progesterone increases libido? Progesterone 11
L Progesterone dosage for dog Animals 8
P Pregnenolone/Progesterone help? Ask For Help or Advice 1
Mito Progesterone suppresses the progression of colonic carcinoma Cancer 4
haidut Stress (cortisol) causes pre-term birth by blocking progesterone Scientific Studies 4
haidut DHT / Progesterone / aspirin treat, cortisol ineffective, PUFA promotes prostate cancer Scientific Studies 1
B 3 Meathead Chemists talk Progesterone Progesterone 28
Drareg Progesterone could reduce the severity of their COVID-19 Articles & Scientific Studies 11
B Progesterone injections for covid 19 Progesterone 0
bogbody Biolabs Pro Progesterone Cream Supplements, Pharmaceutical Drugs 11
A Finasteride vs Progesterone Hair & Nails 15
Sam321 Progesterone: starting dose for resolving insomnia Ask For Help or Advice 14
E Can Pregnenolone replace Progesterone temporarily during Pregnancy? Female Issues 1
V Pregnenolone, Progesterone, DHEA supplementation advice Supplements, Pharmaceutical Drugs 12
ddjd progesterone causing headaches + poor sleep. pregnenolone completely relieves symptoms Ask For Help or Advice 11
theantagonist Muscle gain log: androsterone + progesterone + pregnenelone + DHEA Exercise 24
poilochio is "visanette" With the active ingredient "2mg dienogest" a synthetic progesterone? Ask For Help or Advice 1
haidut’s labrat Your experience with progesterone, T3/T4 and pregnenolone Health 19
Mauritio 5aOHP -Progesterone and DHT mix ? Has anybody heard about this steroid? Supplements, Pharmaceutical Drugs 9
haidut Progesterone and allpregnanolone are protective against COVID-19 Scientific Studies 15
Insomnia Progesterone first time experiment, what is going on?!? Experiments 6
A Farmacautical Progesterone Ask For Help or Advice 2
GuiCHE DIY Topical Progesterone? Supplements, Pharmaceutical Drugs 2
GuiCHE New To "Peating" - Need Advice On Progesterone Supplements, Pharmaceutical Drugs 16
Julian Random Pains After Taking Progesterone Supplements, Pharmaceutical Drugs 2
GorillaHead Low Dose Progesterone And Side Effects? Thyroid and Hormones 24
B Progesterone To Stop Male Facial Hair Growth? Hormones 27
R J MCT In Progesterone Health Natura 17
noqcks Progesterone Causing Hair Loss? Ask For Help or Advice 7
R J Canadian Group Buy For Kenogen Progesterone Progesterone 4
noqcks Progesterone Constipation Ask For Help or Advice 2
Highserotonin90 Progesterone Cream On Scrotum Progesterone 46
Atman Progesterone Use During Pregnancy And The Effects On Child Intelligence Polls 9
haidut Progesterone - Not A Sex Hormone, But A Systemic Health Hormone Scientific Studies 60
S Does Vitamin C Increase Progesterone? Vitamins 12
U Anti-Peat Progesterone-positive Cancer? Debate - Anti-Peat 1
ecstatichamster Ray Peat Interview #35: CO2, Ketosis, And Mitochondria | PUFA, Sugar, Iron, And AGEs | Progesterone And Cell Stabil Danny Roddy 21
Opioidus A List Of Things That Lower Or Antagonize Progesterone Selectively? Male Issues 1
Z Low Progesterone Coming Off Birth Control Female Issues 0
F Can You Build Tolerance With Daily Usage Of 5-10mg Of Progesterone Cream? Progesterone 13
J Forefront Health - Molecular Progesterone Complex Female Issues 6
D Progesterone, No Appetite, Nausea, Hypoglycemia Progesterone 2
Mito Progesterone In The Brain: Hormone, Neurosteroid And Neuroprotectant Progesterone 0
C Vit E Or Progesterone Reducing Face Bloating Ask For Help or Advice 6
C Elevated Progesterone? Ask For Help or Advice 15
Cecilia L. High Pregnenolone, High Progesterone, High Estradiol, Low D Female Issues 13
H Is It Safe To Give A 4 Year Old Girl Progesterone? Email Advice 15
C Progesterone + DHEA Dosages In Young Person Ask For Help or Advice 15

Similar threads