murdoc
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- Oct 13, 2014
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Saturated free fatty acids, palmitic acid and stearic acid, induce apoptosis by stimulation of ceramide generation in rat testicular Leydig cell - ScienceDirect
In men, obesity has generally been associated with reduced plasma testosterone levels and with elevation of the plasma free fatty acids (FFAs). In this study, we investigated the effects of saturated FFAs including palmitic acid (PA) and stearic acid (SA), and polyunsaturatedFFA arachidonic acid (AA) on the survival of rat testicular Leydig cell cultured in vitro. PA and SA markedly suppressed Leydig cell survival in a time- and dose-dependent manner. In contrast, AA stimulated the cell proliferation at 5–10 times of physiological concentration. The suppressive effect of PA and SA on cell survival was caused by apoptosis evidenced by DNA ladder formation and Annexin V-EGFP/propidium iodide staining of the cells. The apoptotic effect of PA was possibly mediated by ceramide generation because it could be completely blocked by ceramide synthase inhibitor fumonisin B1 and exogenous ceramide itself could directly induce apoptosis in vitro. Surprisingly, the apoptosis induced by PA could be partly prevented by AA. These results indicate that PA and SA induce apoptosis in testicular Leydig cells by ceramide production and these apoptotic effects may be a possible mechanism for reproductive abnormalities in obese men, and AA can partly prevent the apoptotic effect induced by saturated FFA.
Obesity is associated with reduced plasma testosterone and elevation of the plasma free fatty acids.
That is in line with Peat.
Than they used rat testicular Leydig cell cultured in vitro and exposed them to palmitic acid, stearic acid arachidonic acid. Not surprisingly AA caused cell proliferation. I think under certain circumstances abnormal cell proliferation is carcinogenic and this forum had made me believe that AA is carcinogenic and SA quite the opposite.
My biochemical understanding is limited, so I have no explanation for the apoptotic effect induced by saturated fatty acid. But given the fact that stearic acid is discussed in this forum because of its positive health effects in terms of visceral fat, CVD and cancer, it seems odd to blame PA and SA for the reproductive abnormalities in obese men. I think the conclusion from this study are pretty far fetched.
Can someone clarify on this?
In men, obesity has generally been associated with reduced plasma testosterone levels and with elevation of the plasma free fatty acids (FFAs). In this study, we investigated the effects of saturated FFAs including palmitic acid (PA) and stearic acid (SA), and polyunsaturatedFFA arachidonic acid (AA) on the survival of rat testicular Leydig cell cultured in vitro. PA and SA markedly suppressed Leydig cell survival in a time- and dose-dependent manner. In contrast, AA stimulated the cell proliferation at 5–10 times of physiological concentration. The suppressive effect of PA and SA on cell survival was caused by apoptosis evidenced by DNA ladder formation and Annexin V-EGFP/propidium iodide staining of the cells. The apoptotic effect of PA was possibly mediated by ceramide generation because it could be completely blocked by ceramide synthase inhibitor fumonisin B1 and exogenous ceramide itself could directly induce apoptosis in vitro. Surprisingly, the apoptosis induced by PA could be partly prevented by AA. These results indicate that PA and SA induce apoptosis in testicular Leydig cells by ceramide production and these apoptotic effects may be a possible mechanism for reproductive abnormalities in obese men, and AA can partly prevent the apoptotic effect induced by saturated FFA.
Obesity is associated with reduced plasma testosterone and elevation of the plasma free fatty acids.
That is in line with Peat.
Than they used rat testicular Leydig cell cultured in vitro and exposed them to palmitic acid, stearic acid arachidonic acid. Not surprisingly AA caused cell proliferation. I think under certain circumstances abnormal cell proliferation is carcinogenic and this forum had made me believe that AA is carcinogenic and SA quite the opposite.
My biochemical understanding is limited, so I have no explanation for the apoptotic effect induced by saturated fatty acid. But given the fact that stearic acid is discussed in this forum because of its positive health effects in terms of visceral fat, CVD and cancer, it seems odd to blame PA and SA for the reproductive abnormalities in obese men. I think the conclusion from this study are pretty far fetched.
Can someone clarify on this?