Methionine Restriction - Ray homers again!

[Sullytex]

Science moves closer to diet that improves metabolic health without sharp cuts in calories

 

[Ideonaut]

url not available

 

[Apple]

We already have lactose free milk
But methionine free milk ... That's an awesome idea!

 

[Sefton10]

url not available

Science moves closer to diet that improves metabolic health without sharp cuts in calories

A diet that improves the biomarkers of metabolic health, and that could potentially slow the aging process, has moved a step closer to reality.

medicalxpress.com

 

[Sefton10]

We already have lactose free milk
But methionine free milk ... That's an awesome idea!

I think Ray's view is that the calcium in milk/dairy negates the impact of methionine. Maybe better to reduce the muscle meats etc. as he says.

 

[baccheion]

The best diet after the plates all fuse seems to be: potatoes, fruits, scallops, and coconut oil. Vegetarian thus far. Maybe some greens for other nutrients if organ meats aren't being used. And calcium to replace shell.

 

[OccamzRazer]

The best diet after the plates all fuse seems to be: potatoes, fruits, scallops, butter, and coconut oil. Vegetarian thus far. Maybe some greens for other nutrients if organ meats aren't being used. And any remaining calcium to replace shell.

Interesting...what nutrients are in potatoes that can't/shouldn't be gotten somewhere else?

 

[baccheion]

Interesting...what nutrients are in potatoes that can't/shouldn't be gotten somewhere else?

You can do a variety of things that work. Here I am specifically combining potatoes, scallops, and coconut oil. Scallops would be added at the end until there was at least 5g leucine (at 70 kg + 16% body fat). All major macros met, leaving greens/organs and shell calcium for remaining nutrients.

 

[Amazoniac]

⮤ Implementation of dietary methionine restriction using casein after selective, oxidative deletion of methionine

Spoiler

"It has been known for 70 years that methionine is readily degraded to methionine sulfoxide by hydrogen peroxide (Matsuo, 1953), and subsequent work showed that methionine and cysteine residues in intact proteins were also subject to oxidation by hydrogen peroxide (Slump and Schreuder, 1973). The authors determined the extent of methionine and cysteine oxidation chemically, followed by biological assay of the nutritive value of the oxidized proteins (Slump and Schreuder, 1973). They concluded that the methionine in casein and fish meal was oxidized almost completely under their conditions. Similarly, Anderson et al. (Anderson et al., 1975) evaluated the ability of rapeseed flour to support the growth of weanling rats after oxidation with hydrogen peroxide. They found that without methionine supplementation (e.g., 0.15%–0.30%), the oxidized protein would not support growth. Other studies showed that tryptophan and lysine were also reduced in casein by hydrogen peroxide (Nielsen et al., 1985), so our goal was to identify the minimal oxidative conditions sufficient to remove methionine and cysteine from casein while causing little additional damage to the protein. Through an iterative process using a range of reaction conditions (e.g., pH, H2O2 concentration, temperature), we identified conditions that were sufficient to reduce methionine and cysteine to near zero while producing a minimum of effects on all other amino acids. Like methionine, tryptophan is very susceptible to oxidation, so it too was totally degraded in the oxidized protein. The only other amino acid that was significantly affected was lysine, and it was reduced by 35% compared with untreated casein (Figure 5)."

"The pelleted casein was [..] divided into two lots. Lot 1 was lyophilized and retained for use in formulation of the casein control diet. Lot 2 was re-suspended in deionized water, adjusted to pH 2.5, and hydrogen peroxide was added to a final concentration of 10%. The suspension was incubated under a fume hood on a heated stir plate for 1 h at 90°C. Hydrogen peroxide was then removed by suction filtration through a sieve with three volumes of deionized water, and the casein was lyophilized and used to formulate the oxidized casein MR diet."

"Based on amino acid analysis of the control and oxidized casein (Figures S1 and S2), the oxidized casein was used to formulate the Ox Cas MR diet by adding back lysine and tryptophan to their original levels in control casein and adding sufficient methionine to bring the final concentration in the diet to 0.17%."

"Metabolites of the sulfur amino acid metabolism pathway previously shown to be affected by dietary MR (Ghosh et al., 2017) were compared, and hepatic levels of serine, ophthalmate, and threonine were comparably increased by the Ele MR and Ox Cas MR diets relative to their corresponding control groups (Figure 2E). In contrast, neither MR diet affected methionine, betaine, or cystathionine levels in the liver (Figure 2E). The two MR diets did produce the expected reductions of hepatic glutathione, glutathione disulfide, and taurine compared with the Ele Con and Cas Con groups, respectively (Figure 2E)."