How to test for PUFA in Tissues

[Velve921]

I am sure this has been discussed in other forums...but what is the best way to medically test for pufa count in bodies tissues? What is a high pufa score in person with lots of PUFAS? What's a low pufa score for someone whose been detoxing pufa or is close to pufa free?

Thanks in advance!!!

 

[Such_Saturation]

Iodine saturation

 

[haidut]

https://raypeatforum.com/forums/posts/97068/ Iodine saturation

How do you do that test? Is this the test where you ingest some iodine and then pee in a cup with starch?

 

[Such_Saturation]

Apparently you add some iodine water to the fat and measure how long it takes for the color to fade. I haven't found any kind of laboratory doing it. It would also be pretty dependent on what you ate the day before, I'm guessing. The easiest thing would probably be http://www.cellbiolabs.com/lipid-quantification-kit

 

[haidut]

https://raypeatforum.com/forums/posts/97134/ Apparently you add some iodine water to the fat and measure how long it takes for the color to fade. I haven't found any kind of laboratory doing it. It would also be pretty dependent on what you ate the day before, I'm guessing. The easiest thing would probably be http://www.cellbiolabs.com/lipid-quantification-kit

Hhm, what about tests like these?
https://www.directlabs.com/TestDetail.aspx?testid=757
https://www.directlabs.com/TestDetail.aspx?testid=545

 

[Such_Saturation]

https://raypeatforum.com/forums/posts/97137/

https://raypeatforum.com/forums/posts/97134/ Apparently you add some iodine water to the fat and measure how long it takes for the color to fade. I haven't found any kind of laboratory doing it. It would also be pretty dependent on what you ate the day before, I'm guessing. The easiest thing would probably be http://www.cellbiolabs.com/lipid-quantification-kit

Hhm, what about tests like these?
https://www.directlabs.com/TestDetail.aspx?testid=757
https://www.directlabs.com/TestDetail.aspx?testid=545

I think a laboratory I go to does something similar. I fear it could be just a few omegas that are tested, presumably because of this ratio craze that goes around. Perhaps a simple syringe in a couple different fat spots would be ideal if there was an actual demand for this data. With some resources it could be created a kit with custom extractors, and the whole thing could be mailed for chromatography or some simpler methods. Something like that biome thing.

 

[Westside PUFAs]

It's a waste of time and money. Those tests are based off of the idea that fatty acids are "vitamins" and if you agree with Ray Peat then you know that there is no such thing as an "essential" fatty acid.

But even if you don't care about "EFA's," it's still futile.

https://raypeatforum.com/forums/posts/97067/ What's a low pufa score for someone whose been detoxing pufa or is close to pufa free?

You can never be "PUFA free." Please read Ray's quotes here: viewtopic.php?f=6&t=7414

Your adipose tissue is mostly concentrated in your belly, thighs, and butt and if you're obese then your adipose tissue in other places besides the belly, thighs, and butt will also be enlarged.

The only way to truly test your fat tissue would be to actually cut it out of your body and have it tested. Are you willing to do that?

This is a real study done on human fat tissue: http://cfpub.epa.gov/ncea/cfm/recordisp ... deid=55204

They did that study on cadavers because well, it's really easy to test the fat tissue of dead people because they're dead.

There is always some amount of triglyceride in the blood.

Look at the three sources of fat:

PUFA (all oils besides coconut, nuts, seeds, soybean, shellfish and finfish, caviar, pork) - Keep low. No need to eat overt PUFA when you can use refined, tasteless coconut oil if you don't like the taste of virgin, or if virgin is allergenic. Many other foods have a mix of PUFA and other fats but should be noted due to having high enough PUFA by themselves.

MUFA (olive, avocado, macadamia nut, palm oil) - Keep low due to accompanied PUFA and simply fat calories. No reason to gorge on olives, avocado, and macadamia nut.

SAFA (cream, any milk/yogurt above skim, cheese, cacao, ruminant meat fat) - Protective/anti-stress and the body safely burns it at rest, mainly the heart muscle and other muscles at rest. It's what the majority of our adipose tissue should be made up of because we're warm-blooded animals. We convert dietary carbohydrate into butyric acid, a SAFA, in our gut. Overt SAFA can be used in context but it's over-consumption in a short amount of time can lead to fat gain and problems with FFA's and the Randle Cycle. The only exceptional SAFA in that reguard would be coconut fat due to it's special instant liver energy properties and metabolism.

If you eat different types of fish and chicken, you're also getting a dose of a mix of all three fats in a chicken or fish based meal.

"Just about everything that goes wrong involves FFA increase. If they are totally saturated fatty acids, such as from coconut oil and butter, those are less harmful, but they still tend to shift the mitochondrial cellular metabolism away from using glucose and fructose and turning on various stress related things; By lowering the carbon dioxide production I think is the main mechanism." - RP

Not many people completely avoid PUFA 100% due to social pressure or just simply from enjoying themselves once in a while. Those social PUFA events add up and with the natural diet you try to eat at home, you're always going to be consuming fat.

 

[sctb]

https://raypeatforum.com/forums/posts/97152/We convert dietary carbohydrate into butyric acid, a SAFA, in our gut.

I think it would be more accurate to say that some dietary fiber and
starch is fermented into butyrate by intestinal bacteria. And since
we're add it, eating butter and parmesan cheese been shown to
increase butyrate concentrations. Good food for colonocytes :)

- Scott

 

[Such_Saturation]

That's why i dismissed blood sampling and suggested syringes...

 

[pboy]

if you are PUFA free indeed, a certificate will come in the mail from ray peat, signed by him with your name on it...if you're lucky he'll show up at your door and shake your hand

this is the only way to tell

 

[Velve921]

Great stuff Westside! Thank you for all your info.

I have been low pufa for 1.5 years and so I guess I'm and to see peats ideals put into reality for myself; however I would be curious to hear more people's experiences who are further along.

Just anxious!