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Gonadin - Liquid Steroid Optimizer For Lab/R&D

Discussion in 'IdeaLabs' started by haidut, Feb 17, 2017.

  1. haidut

    haidut Member

    Joined:
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    As I mentioned in a few threads before, I have always been interested in the so-called "reproductive aging", both in males and females. There is solid evidence that gonadal function declines with age and as such the synthesis and circulating levels of protective steroids like testosterone (T), progesterone (P4), DHEA, and pregnenolone (P5) declines in both men and women. Initially, this decline is buffered to a degree by increased adrenal activity and DHEA synthesis. But with advancing age, the adrenal layers that synthesis DHEA tend to atrophy, leaving cortisol and estrogen to rule unopposed.
    Actually, total T levels in men do not decline with age but this appears to be due to decreased clearance, while de-novo synthesis in the Leydig cells does decline quite markedly with aging, in parallel to falling levels of pregnenolone, progesterone and DHEA. In women, after menopause progesterone, pregnenolone and DHEA levels decline just as sharply as they do in males.
    Interestingly, this decline in endogenous synthesis does not seem to be due to some kind of damage or atrophy of the gonads. To the contrary, cell extracted from "old" human gonads perform just as well as cells from "young" gonads when placed in optimal laboratory conditions with sufficient amount of precursors and enzyme co-factors. This shows that the decline in gonadal function is...well...functional and not structural. So, I have been searching for substances that can help restore such optimal endogenous environment to gonads. Thyroid hormone and high NAD/NADH ratio definitely seem to play a role, but there are additional pathways that appear to be involved and administering thyroid hormone is not always optimal or even desirable. One of the more interesting studies I stumbled upon (which has been posted on the forum) demonstrated that administration of vitamin K2 (MK-4) to old rats doubled their testosterone levels without any addition of other stimulating agents like thyroid hormone, or NAD, or even precursors like pregnenolone. More importantly, Vitamin K2 did not affect pituitary hormones like LH, which are typically involved in steroidogenesis. Furthermore, vitamin K1 did not have a testosterone boosting effect so this is yet another reason for chosing MK-4 over K1 (as this question keeps coming up on the forum).
    Menaquinone-4 enhances testosterone production in rats and testis-derived tumor cells

    That vitamin K2 study apparently got the authors thinking what could be the reason behind this effect of boosting testosterone synthesis. Given that the quinone structure is the same in both K1 and K2, the only difference is the side chain. Vitamin K2 contains geranylgeraniol (GGOH) as a side chain, while vitamin K1 does not. So, the same authors decided to do another study using just GGOH as well as a few other related substances and examine their effects on steroidogenesis.
    http://www.tandfonline.com/doi/abs/10.1080/09168451.2015.1123612?src=recsys&journalCode=tbbb20

    As expected, GGOH did boost testosterone levels and in a concentration about the same as the one used in the first study with MK-4 - i.e. 10μM/L - 30μM/L. It also increased the synthesis of progesterone, which is an important direct precursor to steroid synthesis in the gonads, including synthesis of T. So, the testosterone-boosting effects of vitamin K2 (MK-4) appear to be due mostly to that side chain and not the quinone structure, which matches the results from the first study. Moreover, another similar terpene known as Phytol (POH) was even more effective than GGOH in boosting synthesis of both testosterone and progesterone in Leydig cells when used in concentrations of 30μM/L (see attached screenshots).

    Furthermore, the same authors published a new study in which they not only reiterated their belief that phytol and GGOH would raise T levels but also did an in vivo study to confirm their results.
    A novel function of geranylgeraniol in regulating testosterone production. - PubMed - NCBI
    "...Testosterone and progesterone levels in I-10 cell culture mediums markedly increased in the presence of phytol and GGPP, but not in the presence of GOH. Meanwhile, FOH enhanced progesterone, but not testosterone levels. These results indicated that phytol and GGOH have similar effects on testosterone and progesterone production although, unexpectedly, GOH did not affect steroid production in I-10 cells. Recent researches have revealed the biological activity of compounds derived from the isoprenoid/cholesterol synthesis pathway. These isoprenoids, including FOH, GOH, and phytol, regulate various biological processes [47–49]. The results indicated that not only GGOH, but other isoprenoid derivatives, can enhance testosterone and progesterone levels, although the mechanisms by which they exert these effects are yet to be clarified."

    "...We also found that dietary supplementation of MK-4 enhanced plasma testosterone levels in rats, without any alternation of plasma LH levels [45]. Based on the results of our cell-based experiments [32], we conducted further experiments on the effects of GGOH on testosterone production in animals. Eight-week-old Wistar male rats were purchased from SLC Japan (Shizuoka, Japan) and fed either GGOH supplemented (48.3 mg/kg of diet) or control diet for 10 days. Growth performance did not differ between both diets, but plasma testosterone levels in GGOH supplemented group were found to be elevated compared to that of control group, indicating that dietary supplementation of GGOH significantly elevates plasma testosterone levels (unpublished data, Figure 4). These findings provide novel mechanistic insights into the process of testosterone production and may be useful for the development of therapeutic strategies to counter age-associated declines in testosterone levels in men. In summary, the novel role of GGOH in steroidogenesis may bring new possibilities and could be useful in the development of therapeutics for the treatment of men with LOH."

    Since the in vitro tests in the previous studies used the same concentration of phytol and GGOH (100 uM/L), we can use the new in vivo study to estimate the HED dose for phytol. The in vivo study used GGOH in a dose of 48.3 mg/kg of diet in rats, which is 4.83 mg/kg of bodyweight, which is an HED of 0.8 mg/kg. Since phytol has slightly higher molar mass than GGOH the HED for phytol would be 0.816 mg/kg and thus the 100mg a dose of Gonadin provides should be more than enough to replicate this study. As you can see from the attached image, T levels doubled and did so in just 10 days of supplementing!

    Phytol - Wikipedia
    Phytol is quite an interesting substances as it is a precursor to both vitamin E and K and is used as raw material by some organisms who can synthesize these vitamins endogenously. More importantly, both of these vitamins have been found to raise T levels in animal (and some human) studies, which seems to be due to the presence of that side chain (derived from phytol) attached to the quinone ring. In addition, it was also discovered that phytol is a potent aromatase inhibitor with an IC50 of just 1μM/L, which is quite easily achievable with the amounts present is our product. In fact, in this study phytol (code-named SA-20 in the study and attached screenshots) was as effective as formestane (abbreviated FOR in the study and attached screenshots) in inhibiting estradiol synthesis when used in concentration of 1μM/L (see attached image). Formestane is a steroidal aromatase inhibitor similar to but more potent than exemestane. Perhaps even more importantly, phytol reduced mRNA expression of aromatase itself for up to 24 hours, which suggests that it acts as a long lasting "suicide aromatase inhibitor" just like formestane. In concentrations of 10μM/L, phytol was again as effective as formestane in decreasing actual aromatase expression. As mentioned above, the 100mg dose of phytol per serving (8 drops) of Gonadin should achieve 30μM/L concentrations, so the methods of the study on aromatase should be quite easily replicated. Overall, the aromatase inhibition effects of phytol could very well be responsible at least partly for the observed increase in testosterone levels, in addition to the stimulation of StAR. I am not aware of any other substance that can both inhibit aromatase (and long-lasting at that) AND promote endogenous testosterone/progesterone synthesis.

    Two natural products, trans-phytol and (22E)-ergosta-6,9,22-triene-3β,5α,8α-triol, inhibit the biosynthesis of estrogen in human ovarian granulosa ... - PubMed - NCBI
    "...Two compounds trans-phytol (SA-20) and (22E)-ergosta-6,9,22-triene-3β,5α,8α-triol (SA-48)-were found to potently inhibit estrogen biosynthesis (IC50: 1μM and 0.5μM, respectively). Both compounds decreased aromatase mRNA and protein expression levels in KGN cells, but had no effect on the aromatase catalytic activity in aromatase-overexpressing HEK293A cells and recombinant expressed aromatase. The two compounds decreased the expression of aromatase promoter I.3/II. Neither compound affected intracellular cyclic AMP (cAMP) levels, but they inhibited the phosphorylation or protein expression of cAMP response element-binding protein (CREB). The effects of these two compounds on extracellular regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinases (MAPKs), and AKT/phosphoinositide 3-kinase (PI3K) pathway were examined."
    "...In the present study out of only 5 terpenoids and 6 steroids examined, one acyclic diterpenoid (SA-20), and one functionalized ergostane steroid, SA-48, potently inhibited estrogen biosynthesis in KGN cells. Currently, these two compounds are the most potent terpenoid and steroid reported to inhibit aromatase in a cell-based assay. Phytol (SA-20) is an acyclic diterpenoid categorized as a branched-chain fatty alcohol (3,7,11,15-tetramethyldexadec 2-en-1-ol). It is found abundantly in nature as part of the chlorophyll molecule, and a relatively high amount of free phytol is present in dairy products (Brown et al., 1993). The finding in this study that phytol is a potent aromatase inhibitor gives new insight on our understanding of the beneficial effects of vegetables and fruit on human health."
    "...We found that SA-20 (phytol) and SA-48 exhibited their inhbitory effect in KGN cells until 12-24 h, indicating that they modulate aromatase at the transcriptional level. This is further supported by the findings that they inhibited aromatase mRNA and protein expression."
    "...In normal breast cells, aromatase expression is primarily derived from the tissue-specific promoter I.4 for transcription, whereas in cells from patients with breast cancer, the expression is primarily derived from the utilization of promoter I.3/II. As a result, estrogen biosynthesis sqitches from regulation by a promoter controlled primarily by glucocorticoids and cytokines to regulation by a promoter controlled through cAMP-mediated pathways by prostaglandin E2 (PGE2), a powerful stimulator of adenylate cyclase (Zhao et al., 1996). Thus, the inhbition of promoter II-driven aromatase expression by means of anti inflammatory cyclooxygenase inhibitors to reduce PGE2 is attracting attention for the tissue specific treatment of breast cancer (Davies et al., 2012)."
    "...Aromatase transcription is primarily controlled by promoter 1.3/II in ovarian granulosa cells. Thus, we examined whether SA-20 and SA-48 exert their inhibitory effects on aromatase transcription through these two promoters. SA-20 and SA-48 at 50uM decreased 20-30% of the promoter I.3 (Fig. 7A). However, at the same concentration (50uM), SA-20 and SA-48 decreased 60%-70% of the promoter II (Fig. 7B). These results indicate that the inhibition of aromatase transcription by SA-20 and SA-48 is mediated through promoter I.3/II, with promoter II playing a more prominent role."

    In summary, phytol seems to be a very versatile substance with a wide array of beneficial effects. As one study aptly summarized these effects include:
    Phytol in a pharma-medico-stance. - PubMed - NCBI
    "...In the pharma-medico viewpoint, PYT and its derivatives have been evident to have antimicrobial, cytotoxic, antitumorous, antimutagenic, anti-teratogenic, antibiotic-chemotherapeutic, antidiabetic, lipid lowering, antispasmodic, anticonvulsant, antinociceptive, antioxidant, anti-inflammatory, anxiolytic, antidepressant, immunoadjuvancy, hair growth facilitator, hair fall defense and antidandruff activities. Otherwise, the important biometebolite of PYT is phytanic acid (PA). Evidence shows PA to have cytotoxic, anticancer, antidiabetic, lipid lowering and aniteratogenic activities. In addition, it may be considered as an important biomarker for some diseases such as Refsum's Disease (RD), Sjögren Larsson syndrome (SLS), rhizomelic chondrodysplasia punctata (RZCP), chronic polyneuropathy (CP), Zellweger's disease hyperpipecolic academia (ZDHA) and related diseases. Thus, phytol may be considered as a new drug candidate."

    Finally, phytol is one of the few known chemicals that raise NAD levels in vivo and thus improve the oxidative state of the organism.
    http://www.tandfonline.com/doi/pdf/10.1271/bbb.130029
    "...In conclusion, we found that phytol increased the blood NAD level via ACMSD protein suppression and mRNA expression in rat liver. It is possible that this mechanism resulted from the activation of PPAR as well of other transcription factors. We will carry out additional studies on the regulation of ACMSD gene expression by phytol to elucidate this issue."


    As the study on POH/GGOH observed, other related terpenes like farnesol or geranylgeranyl diphosphate (GGPP) were not effective in stimulating steroid synthesis, even though farnesol did boost progesterone synthesis but was much weaker compared to GGOH or POH.
    "...We previously reported that menaquinone-4, one of vitamin K2, stimulates testosterone production in I-10 cells via regulation of PKA activity13) and presumed that unsaturated side chain of menaquinone-4, geranylgeranyl group, may be important to express this activity. And we also found that both menaquinone-4 and GGOH have anti-inflammatory activity in lipopolysaccaride-induced inflammation model.5,15) Here, we firstly demonstrated that GGOH stimulates steroidogenesis via activation of cAMP/PKA pathway. Testosterone production is regulated by a complex signaling cascade, with cholesterol transported into mitochondria to initiate steroidogenesis in Leydig cells. Progesterone is an important hormone that is converted to several steroids–including testosterone–and is secreted by I-10 cells.16,17) GGOH stimulated both testosterone and progesterone productions (Fig. 1). Therefore, we measured progesterone levels as a precursor of testosterone in further experiments in Figs. 3(B), 5, and 6(C) to clarify detailed mechanism of GGOH in steroidogenesis."

    As the study describes, endogenous synthesis of steroids by the gonads (and actually peripheral cells as well) is rate-limited by the expression and activity of the so-called Steroid Acute Regulatory Protein (StAR).
    Steroidogenic acute regulatory protein - Wikipedia

    The higher the levels of this protein, the more steroids are synthesize from cholesterol and (maybe even more importantly) from other precursors as well. Cholesterol is not the only precursor to steroid synthesis and we are just beginning to understand the role of these other precursors. But as far as the levels of StAR, GGOH (and POH) increased those level more than 6-fold. I am not aware of any other substance with such dramatic effects on upregulating StAR. Finally, contrary to what other studies have suggested and what some forum members have asked for, PDE levels did not correlate with steroid synthesis. So, a PDE inhibitor will probably not be as effective as a steroid booster compared to GGOH or POH.
    "...On the other hand, we did not find any evidence of a role for PDE in steroidogenesis. StAR functions in the rate-limiting step of steroid production in Leydig cells and is required for the delivery of cholesterol to the inner mitochondrial membrane.26) We showed that GGOH treatment increased the RNA and protein levels of StAR, which acts downstream in the cAMP/PKA pathway. Taken together, our results demonstrate that GGOH enhances testosterone and progesterone production in I-10 cells via induction of cAMP/PKA signaling. These findings provide novel mechanistic insight into the process of steroidogenesis and may be useful for the development of therapeutic strategies to counter age-associated declines in testosterone levels in men."

    As you can see, the authors feel confident that the effects of POH and GGOH may be useful as therapy for declining levels of endogenous steroidogenesis in males. While the study was focused on males, there is evidence that the same steroidogenic process and rate-limiting steps are present in females. Thus, POH or GGOH should have similar beneficial effects in females which is supported by the fact that POH/GGOH boosted progesterone synthesis as much as testosterone. So, I am hoping that GGOH/POH could help both andropause and menopause.

    Inspired by the study above, I searched for other terpenes known to be involved in steroidogenesis and stumbled upon squalene.
    Squalene - Wikipedia
    Squalene is also a very interesting substances as it is actually a precursor to all steroids, including lanosterol and vitamin D. As it seems, squalene (in doses matching the required dose to achieve the optimal concentrations of POH and GGOH in the above study) boosted testosterone levels and dramatically improved the reproductive performance (fertility) in boars. While the study did not delve into the mechanism of action of squalene, it likely involves both its role as steroid precursors and its ability to boost mitochondrial function.
    Feeding with supplemental squalene enhances the productive performance in boars. - PubMed - NCBI
    "...Feeding with squalene at 10 mg/kg/day neither significantly increase the reproductive performance and serum testosterone levels, nor reduced the levels of leptin in boars. Surprisingly, feeding with supplemental squalene at 20 or 40 mg/kg/day significantly improved the reproductive performance as evidenced by dramatically reduced the time for mating, increased semens' volume and motility, and increased the size of litter as compared with that in controls. Furthermore, feeding with a higher dose of squalene significantly reduced the levels of serum leptin, accompanied by elevated levels of testosterone, as compared with that in controls. These data clearly indicate that feeding with squalene can improve the reproductive performance in boars. Therefore, dietary supplementation with squalene may increase the productivity of pig industry and potentially benefit for other animal production."

    And here is another in vivo study (in rats) also showing testosterone increaseing, blood pressure lowering, and leptin lowering effects of squalene.
    Influence of squalene feeding on plasma leptin, testosterone & blood pressure in rats. - PubMed - NCBI
    "...RESULTS: Following squalene feeding, BP and body weight gain were lower in the squalene group. BP was significantly lower from 47 days of age in squalene fed group compared to controls. The levels of plasma leptin, glucose, cholesterol and triglycerides were significantly lower in squalene fed rats than those from the control group at 51 and 75 days of age. However, testicular weights (only 75 days) and testosterone levels were significantly higher in rats from the squalene group than those from the control group at days 51 and 75."

    Squalene is also known to protect from lipophilic xenobiotics like BPA and other aromatic hydrocarbons produced by modern industries, protect from radiation toxicity, reduce cholesterol levels, improve skin health, protect from certain cancers, reduce lipid and muscular lipid accumulation, prevent CVD, and others.

    In addition to the phytol and squalene, I have been researching the steroidal effects of various fatty acids and their esters. There is considerable evidence that saturated fats increase binding of androgens to their "receptors" and also increase androgen synthesis. Most of the studies on androgenic effects of saturated fat were done using the unmodified versions of such fats like palmitic and butyric acids. However, a recent study found that the methyl esters of some fatty acids have an even more potent androgenic effects and in even raise testosterone levels in castrated rats. While the effects of the fatty acid esters were not quite as potent as testosterone (T), the effects were not far behind those of dose of T administration. Also, the doses of fatty acid esters used was quite low and there is likely to be a dose-dependent effect, so higher doses would be more potent and may reach the effectiveness of T. Thus, using specific esters of fatty acids like palmitate and oleate may provide another non-steroid method of increasing gonadal activity and raising serum levels of androgens.
    Androgenic effect of honeybee drone milk in castrated rats: roles of methyl palmitate and methyl oleate. - PubMed - NCBI
    "..."...NMR and MS measurements after the second fractionation revealed MP and MO in the last active fraction (II/E) of the raw DM. Although MO alone had no effect on androgen-sensitive organs, MP (similarly to raw DM) increased the weights of the androgen sensitive organs (except the prostate) and these effects were flutamide-sensitive. Palmitate is known to play a role in steroidogenesis: it is able to increase the DHEA level through its CYP17 activity (Bellanger et al., 2012). A fatty acid infusion has been reported to elevate human androgen production in both sexes (Mai et al., 2006, 2008). MP was recently proved to inhibit carrageenan-induced paw oedema by reducing the prostaglandin E2 level (Saeed et al., 2012), an effect which might indicate a steroidogenesis-inducing property. Since DHEA alone has a weak androgenic effect, the putative DHEA-elevating effect of MP may explain in part the response of androgen-sensitive organs. The androgenic dose (25 μg/kg) of MP alone did not alter the plasma testosterone level, but its combination with MO in high dose exhibited plasma testosterone-increasing effect, similarly to the action of raw DM. It is known that oleic acid has a weak 5-α- reductase inhibitory effect, preventing testosterone conversion to dihydrotestosterone, whereas the esterified analogues of oleic acid (like MO) are ineffective in this respect (Liu et al., 2009). As yet we have no explanation as to why the combination of MP and MO increases the plasma testosterone level in rat. Nevertheless, we have clearly shown that these two compounds have a major role in the main androgenic action of DM. Further studies are required to clarify the androgenic mechanisms of action of MO and MP.""

    Interestingly, it is worth noting that when the palmitate ester (an SFA) was used on its own it only had androgenic effects. However, combined with an oleate ester (a MUFA) it also raised T levels in the castrated rats. This synergistic effect of saturated and mildly unsaturated substances has been seen in many other studies with steroids where the effects of combining a saturated steroids like androsterone are much more potent when it is combined with an unsaturated steroid like DHEA. The same effects have been seen with combinations of T/DHT and DHT/DHEA. So, I doubt the findings of this study are a coincidence, and this is what led me to add both the palmitate and oleate esters to the product.

    In addition to methyl palmitate and methyl oleate, another SFA which has been found to have an androgenic effect is caprylic acid. I have posted other studies about caprylic (octanoic) acid in regards to its anti-cancer effects and anti-cortisol effects, which would be quite expected if it is indeed androgenic.
    Novel phytoandrogens and lipidic augmenters from Eucommia ulmoides
    "...Subsequent 1H NMR and GC analyses of active fraction CB showed the major presence of the 8-carbon polysaturated fatty acid, caprylic acid, along with other lipids (figure (figure1313 and table table1).1). Bioassays using pure caprylic acid and other polysaturated fatty acids (PFAs) correlated with the augmenting effect of E. ulmoides on the AR (figure (figure14)14) in varying degrees. Ethanolic extract of coconut (Cocos nucifera) flesh, rich in C-8 caprylic acid and other polysaturated fatty acids [11], replicated the hormone potentiating effect of both E. ulmoides extract and pure caprylic acid in AR bioassays (data not shown)."

    Thus, in light of the evidence for beneficial effects of squalene, phytol, and the fatty acid esters I decided to release the product Gonadin. Its primary purpose is endogenous steroid optimization, however, it may be able to do much more judging from the studies in the "References" section below. The doses of squalene and phytol in Gonadin are based on the study of squalene in boars (40mg/kg of diet) and the optimal phytol concentration (30 μM/L) from the in vitro study and the vitamin K2 study. In both cases, about 100mg of each is required to replicate the design of both studies. Like any unsaturated compounds, phytol and squalene have a potential for side effects. Animal studies have shown that at very high doses (10-15 times higher than doses in Gonadin), squalene and phytol can cause liver enlargement and their toxicity profile resembles that of vitamin A. So, it is probably best to stick to the 100mg amounts daily of each substance unless there is a very good reason for going with higher doses. To further lower the potential risk of such side effects related to the unsaturatedness, Gonadin contains vitamin E similar to the common practice to add vitamin E to vitamin A supplements to both prevent peroxidation of the vitamin A and protect from its possible toxicities.

    *******************************************************************************
    Gonadin is a chemical for optimizing endogenous steroid synthesis. Its ingredients phytol and squalene have been shown in scientific studied to both promote the synthesis of steroids from endogenous precursors like cholesterol, as well as potentially serve as steroid precursors themselves. In addition, squalene and phytol have been shown to possess a variety of beneficial effects in animal (and some human) studies including restoring mitochondrial function in aged organisms, lowering cholesterol levels and triglycerides, protection from radiation-induced injury and immunosuppression, improvement of bile acid synthesis, antimicrobial, cytotoxic, antitumorous, antimutagenic, anti-teratogenic, antibiotic-chemotherapeutic, antidiabetic, lipid lowering, antispasmodic, anticonvulsant, antinociceptive, antioxidant, anti-inflammatory, anxiolytic, antidepressant, immunoadjuvancy, hair growth facilitator, hair fall defense and antidandruff activities, etc.


    Units per container: about 30
    Unit size: 8 drops
    Each unit contains the following ingredients:

    Squalene: 100mg
    Phytol: 100mg
    Methyl caprylate: 20mg
    Methyl palmitate: 3.3mg
    Methyl oleate: 3.3mg

    Other ingredients: tocopherols, MCT
    *******************************************************************************

    References:

    1. Miscellaneous
    Absorption and metabolic fate of dietary 3H-squalene in the rat. - PubMed - NCBI (squalene)
    Dietary squalene increases tissue sterols and fecal bile acids in the rat. - PubMed - NCBI (squalene)
    Metabolism of squalene in human fat cells. Demonstration of a two-pool system. - PubMed - NCBI (squalene)
    Fate of intravenously administered squalene in the rat. - PubMed - NCBI (squalene)
    Effects of prolactin, progesterone, and 17beta-hydroxy-5alpha-androstan-3-one on squalene production by the preputial gland of the immature female ... - PubMed - NCBI (squalene)
    Squalene inhibits sodium arsenite-induced sister chromatid exchanges and micronuclei in Chinese hamster ovary-K1 cells. - PubMed - NCBI (squalene)
    Biological importance and applications of squalene and squalane. - PubMed - NCBI (squalene)
    Squalene as novel food factor. - PubMed - NCBI (squalene)
    Squalene: A natural triterpene for use in disease management and therapy. - PubMed - NCBI (squalene)
    The protective role of squalene in alcohol damage in the chick embryo retina. - PubMed - NCBI (squalene)
    The inhibitory effects of squalene-derived triterpenes on protein phosphatase PP2A. - PubMed - NCBI (squalene)
    Fate of intravenously administered squalene and plant sterols in human subjects. - PubMed - NCBI (squalene)
    Feeding with supplemental squalene enhances the productive performance in boars. - PubMed - NCBI (squalene)
    Squalene promotes the formation of non-bilayer structures in phospholipid model membranes. - PubMed - NCBI (squalene)
    Postabsorptive metabolism of dietary squalene. - PubMed - NCBI (squalene)
    Studies on the conversion of squalene to sterol with rat liver enzymes. - PubMed - NCBI (squalene)
    Testicular sterols. VI. Incorporation of mevalonate into squalene and sterols by cell-free preparations of testicular tissue. - PubMed - NCBI (squalene)
    Evaluation of Antioxidant Activity of Phytol Using Non- and Pre-Clinical Models. - PubMed - NCBI (phytol)
    Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa. - PubMed - NCBI (phytol)
    In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium. - PubMed - NCBI (phytol)
    Phytol in a pharma-medico-stance. - PubMed - NCBI (phytol) (hair-growth effects)
    In vitro anti-quorum sensing activity of phytol. - PubMed - NCBI (phytol)
    Phytol derivatives as drug resistance reversal agents. - PubMed - NCBI (phytol)
    Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical disease Schistosomiasis mansoni. - PubMed - NCBI (phytol)
    Phytol metabolites are circulating dietary factors that activate the nuclear receptor RXR. - PubMed - NCBI (phytol)
    Effects of dietary phytol and phytanic acid in animals. - PubMed - NCBI (phytol)
    Effects of phytol, a branched, long-chain aliphatic alcohol, on biochemical values and on hepatic peroxisomal enzymes of rats. - PubMed - NCBI (phytol)


    2. Metabolism/Mitochondria/Diabetes
    Protective effect of dietary squalene supplementation on mitochondrial function in liver of aged rats. - PubMed - NCBI (squalene)
    [SQUALENE: PHYSIOLOGICAL AND PHARMACOLOGICAL PROPERTIES]. - PubMed - NCBI (squalene)
    Phytol/Phytanic acid and insulin resistance: potential role of phytanic acid proven by docking simulation and modulation of biochemical alterations. - PubMed - NCBI (phytol)
    Phytanic acid, but not pristanic acid, mediates the positive effects of phytol derivatives on brown adipocyte differentiation. - PubMed - NCBI (phytol)
    Effects of phytol, a branched, long-chain aliphatic alcohol, on biochemical values and on hepatic peroxisomal enzymes of rats. - PubMed - NCBI (phytol)
    Phytol increases adipocyte number and glucose tolerance through activation of PI3K/Akt signaling pathway in mice fed high-fat and high-fructose diet. - PubMed - NCBI (phytol)

    2. CVD/Cholesterol
    Metabolic variables of cholesterol during squalene feeding in humans: comparison with cholestyramine treatment. - PubMed - NCBI (squalene)
    Sterol synthesis from biliary squalene in the jejunal mucosa of the rat in vivo. - PubMed - NCBI (squalene)
    The effect of the administration of squalene and other hydrocarbons on cholesterol metabolism in the rat (squalene)
    Lipidaemic effects of tocotrienols, tocopherols and squalene: studies in the hamster. - PubMed - NCBI (squalene)
    Effectiveness and safety of low-dose pravastatin and squalene, alone and in combination, in elderly patients with hypercholesterolemia. - PubMed - NCBI (squalene)
    Dietary squalene increases high density lipoprotein-cholesterol and paraoxonase 1 and decreases oxidative stress in mice. - PubMed - NCBI (squalene)
    Squalene ameliorates atherosclerotic lesions through the reduction of CD36 scavenger receptor expression in macrophages. - PubMed - NCBI (squalene)
    Squalene in a sex-dependent manner modulates atherosclerotic lesion which correlates with hepatic fat content in apoE-knockout male mice. - PubMed - NCBI (squalene)
    Cardioprotective effect of squalene on lipid profile in isoprenaline-induced myocardial infarction in rats. - PubMed - NCBI (squalene)
    Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats. - PubMed - NCBI (squalene)
    Amaranth squalene reduces serum and liver lipid levels in rats fed a cholesterol diet. - PubMed - NCBI (squalene)
    Further in vitro evaluation of antiradical and antimicrobial activities of phytol. - PubMed - NCBI (phytol)


    3. Inflammation
    Dietary squalene supplementation improves DSS-induced acute colitis by downregulating p38 MAPK and NFkB signaling pathways. - PubMed - NCBI (squalene)
    Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. - PubMed - NCBI (phytol)
    Antinociceptive and Antioxidant Activities of Phytol In Vivo and In Vitro Models. - PubMed - NCBI (phytol)
    Anti-scratching behavioral effect of the essential oil and phytol isolated from Artemisia princeps Pamp. in mice. - PubMed - NCBI (phytol)


    4. Chemoprevention
    Potentiation by squalene of the cytotoxicity of anticancer agents against cultured mammalian cells and murine tumor. - PubMed - NCBI (squalene)
    Antitumor activity of squalene-treated cell-wall skeleton of Nocardia rubra in mice. - PubMed - NCBI (squalene)
    Chemopreventive effect of squalene on colon cancer. - PubMed - NCBI (squalene)
    The preventive and therapeutic potential of the squalene-containing compound, Roidex, on tumor promotion and regression. - PubMed - NCBI (squalene)
    Inhibition by squalene of the tumor-promoting activity of 12-O-tetradecanoylphorbol-13-acetate in mouse-skin carcinogenesis. - PubMed - NCBI (squalene)
    Modulation of doxorubicin-induced genotoxicity by squalene in Balb/c mice. - PubMed - NCBI (squalene)
    Squalene: potential chemopreventive agent. - PubMed - NCBI (squalene)
    The possible role of squalene as a protective agent in sebum. - PubMed - NCBI (squalene)
    An insight into the cytotoxic activity of phytol at in vitro conditions. - PubMed - NCBI (phytol)
    Diol- and triol-types of phytol induce apoptosis in lymphoid leukemia Molt 4B cells. - PubMed - NCBI (phytol)
    Phytol induces programmed cell death in human lymphoid leukemia Molt 4B cells. - PubMed - NCBI (phytol)


    5. Skin health
    The possible role of squalene as a protective agent in sebum. - PubMed - NCBI (squalene)
    High-dose squalene ingestion increases type I procollagen and decreases ultraviolet-induced DNA damage in human skin in vivo but is associated with... - PubMed - NCBI (squalene)
    Squalene as a target molecule in skin hyperpigmentation caused by singlet oxygen. - PubMed - NCBI (squalene)
    Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. - PubMed - NCBI (squalene)


    6. Radiation Protection
    Radioprotection of mice by dietary squalene. - PubMed - NCBI (squalene)


    7. Neurotransmitters/Neuroprotection
    Effects of squalene/squalane on dopamine levels, antioxidant enzyme activity, and fatty acid composition in the striatum of Parkinson's disease mou... - PubMed - NCBI (squalene)
    Phytol a Natural Diterpenoid with Pharmacological Applications on Central Nervous System: A Review. - PubMed - NCBI (phytol)
    Anxiolytic-like effects of phytol: possible involvement of GABAergic transmission. - PubMed - NCBI (phytol)
    Anticonvulsant effect of phytol in a pilocarpine model in mice. - PubMed - NCBI (phytol)
     

    Attached Files:

  2. tca300

    tca300 Member

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    This looks fantastic! Thank you very much! My lab rats are dancing with excitement.
     
  3. Constatine

    Constatine Member

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    Impressive as ever.
     
  4. Dan Wich

    Dan Wich Member

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    Aww yeah, I never catch these right when they launch. Intriguing stuff.

    Here's the "title-ified" references:

    1. Miscellaneous

    2. Metabolism/Mitochondria/Diabetes

    3. CVD/Cholesterol

    4. Inflammation

    5. Chemoprevention

    6. Skin health

    7. Radiation Protection

    8. Neurotransmitters/Neuroprotection
     
  5. grenade

    grenade Member

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    Wow, you're on a roll! Good stuff, Haidut.
     
  6. OP
    haidut

    haidut Member

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    Yeah, I am pretty excited too. If it turns out that this can indeed help age-related hypogonadism without any shutdown or change in LH levels it would be pretty big deal. I suspect adding some pregnenolone and/or DHEA may make it even more powerful.
     
  7. OP
    haidut

    haidut Member

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    Thanks! Btw, two more supplements coming next week so should be pretty productive month :):
     
  8. grenade

    grenade Member

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    Can't wait to see them!
     
  9. OP
    haidut

    haidut Member

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    Thanks Dan! I wonder why does the forum sometimes fetch the titles itself and why sometimes it seem to give up mid-way through pulling them. Is there a limit on how many titles it would pull automatically? Maybe @charlie would know?
     
  10. Dan Wich

    Dan Wich Member

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    I can't remember the exact details, but while there was a couple things Charlie could tweak, it never seemed to make it through big lists of links.
     
  11. Koveras

    Koveras Member

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    @haidut

    When you first mentioned squalene as a possible component of TocoVit, I recalled a caution from Andrew Kim:

    "Free fatty acids and squalene are major lipids that make up sebum. Squalene is highly unsaturated in structure and highly susceptible to peroxidation and photodegradation. The byproducts, squalene peroxides, promote acne, roughening of skin, and wrinkling.5,6 The free fatty acids, when polyunsaturated, degenerate to promote the peroxidation of nearby lipids, including squalene, whereas saturated fats do not."

    Not sure on the current state of the research though
     
  12. PUTFOT

    PUTFOT Member

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    If I'm not mistaken these are all natural chemicals?
     
  13. acrylic

    acrylic Member

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    @haidut any information on the safety of these compounds in humans?
     
  14. TubZy

    TubZy Member

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    dude, I love you, no homo:)...but seriously I'm extremely excited! I respond very well to K2 (it shoots my libido up big time) so this is very interesting to me and not only that but it can increase the function of the gonads and provide skin and hair benefits? No brainer...ordered.

    I'll log it when I get it.

    And no DMSO :D
     
  15. LukeL

    LukeL Member

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    @haidut do you think this would have an effect on a rat with high FSH?
     
  16. Dante

    Dante Member

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    Are they also going to be directly androgenic ?
     
  17. Constatine

    Constatine Member

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    Would it be better to apply this on the testicles (of a male rat) or will any area work just as well?
     
  18. Agent207

    Agent207 Member

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    But is it actually squalene or squalane containing? Some of the studies linked here point out to both indistinctly. From what I read it seems to be a strong generalized ethic concern with shark derived squalene, while the squalane being the better approach for skin hydration.


    "Squalane is derived by hydrogenation of squalene. It is naturally present in the skin lipid barrier of plants, animals and humans, preventing moisture loss while restoring skin’s suppleness and flexibility.
    Due to the complete saturation of squalane, it is not subject to auto-oxidation. This coupled with lower costs make it desirable for cosmetics where it is used as an emollient and moisturizer.
    The EU took steps to ban targeted deep‐sea shark fisheries back in 2010. However, consumers willing to buy ethical products cannot choose specific plant based squalane ones because differentiation between the substance of origin is not required in labeling requirements."

    Squalane versus squalene, are you aware of what you may be paying for?
     
  19. Wagner83

    Wagner83 Member

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    Do we have any idea on how much progesterone would be boosted from this product?
    Do you know how much squalene there is in tocovit yet?
     
  20. Agent207

    Agent207 Member

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    Well, some comments
    In fact, it should be stated that it was observed to be more effective at 30μM. Otherwise, at 10μM the outcome was worse than GGOH and close to control.
    What lead me to the question, about how much is the dose required to reach 30μM/L concentration?


    So for testosterone levels to increase it took higher dose than 40mg/kg/day. For average 80kg male this would mean a dose higher than 3gr. of squalene.
     
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