Glycine and endotoxin

Edward

Member
Joined
Apr 20, 2013
Messages
134
Age
37
A diet containing glycine improves survival in endotoxin shock in the rat
http://ajpgi.physiology.org/content/271/1/G97.short

In this study, we investigated the effects of a glycine-containing diet (5%) on mortality and liver injury due to intravenous injection of endotoxin [Escherichia coli lipopolysaccharide (LPS)] in Sprague-Dawley rats in vivo. Fifty percent of the rats fed control diet died within 24 h after an intravenous injection of LPS (10 mg/kg), whereas feeding the rats glycine totally prevented mortality and markedly reduced an LPS-induced elevation of serum transaminase levels, hepatic necrosis, and lung injury. The elevation in serum tumor necrosis factor-alpha (TNF-alpha) due to LPS was also blunted and delayed significantly by glycine feeding. In a two-hit model (hepatic ischemia-reperfusion and injection of sublethal LPS), all rats fed control diet died, whereas 83% of glycine-fed animals survived with a significant reduction in transaminases and improved liver and lung histology. LPS elevated intracellular Ca2+ concentration ([Ca2+]i) in cultured Kupffer cells, an effect blocked almost completely by glycine. Glycine most likely reduces injury and mortality by preventing the LPS-induced elevation of [Ca2+]i in Kupffer cells, thereby minimizing toxic eicosanoid and cytokine production.


Background and aim: There is substantial experimental evidence that the amino acid glycine may have a role in protecting tissues against insults such as ischemia, hypoxia and reperfusion. Our aim was to investigate the ability of the amino acid glycine to prevent hepatic damage induced by injection of lipopolysaccharide and d-galactosamine (d-Gal), to modulate pro- and anti-inflammatory cytokine levels, and to improve survival.

Methods: Mice were challenged with an intraperitoneal injection of d-Gal (16 mg/mouse) and lipopolysaccharide (LPS, 1 μg/mouse). The intervention group also received an intraperitoneal injection of glycine (150 mg/kg) in two doses: 24 h before and just after LPS challenge. Serum cytokine levels were measured 2 h after challenge, and liver enzymes and histology were determined 16 h after LPS. Separate groups of mice received the same treatment and the survival rate was determined 24 h and ten days after endotoxin administration. In in vitro experiments, cultured mononuclear cells were stimulated by LPS, and TNF-α and IL-10 secretion were measured, in the presence or absence of glycine.

Results: In the glycine-treated mice, the serum levels of liver enzymes and TNF-α, the histologic necroinflammation score and the mortality rate were significantly reduced compared to control mice (P<0.001). Serum IL-10 levels in the glycine-treated mice were increased (P<0.01). In vitro studies in cultured lymphocytes isolated from either normal or glycine pretreated mice, demonstrated a significant and dose-dependent inhibition of LPS-induced TNF-α secretion and increase in IL-10 response after treatment with glycine (P<0.01). In conclusion, glycine reduces hepatic damage and improves survival rate in this mouse model of endotoxemia. The protective effect of glycine is associated with modulation of TNF-α and IL-10 secretion.
 
Similar threads
Thread starter Title Forum Replies Date
M A Choline-rich Diet (and Glycine) Improves Survival In A Rat Model Of Endotoxin Shock Scientific Studies 0
zarrin77 Glycine (and Serine) Have Potent “anti-depressive” Effects In Rodent Endotoxin Model Scientific Studies 0
haidut Glycine Is An Endotoxin (TLR4) Antagonist Scientific Studies 36
haidut Glycine (and leucine) can treat fatty liver (NAFLD and NASH) Scientific Studies 3
haidut Glycine has a universal anti-viral effect Scientific Studies 38
Lokzo Glycine Reduces NAFLD by modulating fatty acid oxidation, glutathione synthesis, and the gut microbiome - 2020 Supplements, Pharmaceutical Drugs 20
Ras Glycine Ameliorates NAFLD By Modulating FAO, Glutathione Synthesis, And The Gut Microbiome Amino Acids 0
johnwester130 Lamb Lungs Surprisingly High In Glycine Meat, Organ Meat, Gelatin, Seafood 5
johnwester130 The Ultiamte Simple Mouthwash With Glycine Powder Oral Health 25
Amazigh Astronomers Report They’ve Detected The Amino Acid Glycine In The Atmosphere Of Venus Miscellaneous Health Discussions 12
M Percentage Of Glycine In Chelated Calcium Ask For Help or Advice 1
M Bad Experience With Glycine Ask For Help or Advice 19
Velve921 What Does Everyone Thing Of Sooma For Sleep? It Has Glycine Ask For Help or Advice 0
explosionlord Aspirin + K2 + Glycine Poor Reaction; Anybody Else? Supplements 8
A Gelatin/Glycine In Ground Beef Meat, Organ Meat, Gelatin, Seafood 7
V Foods With A Good Glycine / Methionine Ratio Diet 1
M Your Glycine Experiences? Ask For Help or Advice 35
Hans The Peat Ratio Of Foods (Glycine Vs Methionine, Cysteine And Tryptophan) Discussing Dietary Models 29
M Do You Feel A Difference From Taking Glycine Or Gelatin Or Aspirin? Ask For Help or Advice 5
Hgreen56 Why Are Gelatin And Glycine Recommended If Glycine Increases Serotonin Levels? Supplements, Pharmaceutical Drugs 10
H Coffee Without Glycine Allergy Diet 2
Ravenslore Why Does My Hair Fall Out If I Don't Take Glycine Daily? Ask For Help or Advice 24
berk Someone Experienced Energy From Glycine? Metabolism 8
michael94 Brain Glycine Levels Not Always Limited To Glycine Intake Meat, Organ Meat, Gelatin, Seafood 2
Jib High-Dose Glycine For OCD And BDD Scientific Studies 4
zarrin77 Glycine Protects Liver Cells From Mercury Toxicity Scientific Studies 1
zarrin77 Glycine Completely Protects Liver Cells From Apoptosis Due To Iron Scientific Studies 1
JoshuaD Glycine And Stress Cortisol, Serotonin, Histamine 44
S Mineral Glycine Chelates Taken In The Morning? Supplements 0
L How To Increase Endogenous Glycine Synthesis? Metabolism 8
GorillaHead Once Again Mitochondrial Dysfunction At The Center Of AGING. Glycine Supplementation Huge Scientific Studies 5
I Glycine Digestion 13
_lppaiva Glycine As A Temporary Alternative To Gelatin, Since No Good Brands Are Available? Supplements, Pharmaceutical Drugs 29
M Taurine Vs. Glycine Regarding Gallstones? Supplements 6
olive Gelatin Enhances Non-gene Iron Uptake, Glycine Does Not Scientific Studies 3
C Pregnenolone + Taurine + Glycine Pregnenolone 4
Gadsie Glycine Inhibits Formation Of Blood Vessels In (colon) Tumors Scientific Studies 1
L Oral Exposure To The Free Amino Acid Glycine Inhibits The Acute Allergic Response In A Model Of Cow' Scientific Studies 13
magnesiumania Glycine, Brands, Dosage Amino Acids 25
R What Am I Making (magnesium, Glycine) Supplements, Pharmaceutical Drugs 2
S Getting Up In The Morning And Glycine Insomnia, Sleep Issues 8
J Glycine And Libido Male Issues 17
cyclops Glycine Vs Powdered Gelatin Diet 45
Lokzo Glyphosate Pretending To Be Glycine: Devastating Consequences Articles & Scientific Studies 46
N During A Fast, Glycine/Niacinamide/Asprin Post Workout? Health 22
RayPeatFan777 Simply Eating Oxtails - Enough Glycine? Meat, Organ Meat, Gelatin, Seafood 27
L Glycine Wake-up After 4 Hours Amino Acids 6
Mito Balancing Methionine And Glycine Amino Acids 6
Captain_Coconut Chamomile Boosts Biosynthesis Of Glycine Amino Acids 12
Captain_Coconut Transdermal Glycine Ask For Help or Advice 5

Similar threads

Top