• Due to excessive bot signups along with nefarious actors we are limiting forum registration. Keep checking back for the register link to appear. Please do not send emails or have someone post to the forum asking for a signup link. Until the current climate changes we do not see a change of this policy. To join the forum you must have a compelling reason. Letting us know what skills/knowledge you will bring to the community along with the intent of your stay here will help in getting you approved.

Estradiol Inhibits Hyaluronic Acid Synthase 1 Expression In Human Vascular Smooth Muscle Cells


Jan 6, 2015
Estradiol inhibits hyaluronic acid synthase 1 expression in human vascular smooth muscle cells. - PubMed - NCBI

Epidemiological and clinical data suggest that estrogen retards the progression of atherosclerosis. This study aims to elucidate whether the phenotypic regulation of human vascular smooth muscle cells (VSMC) by estrogen may involve effects on the hyaluronan matrix. VSMC were synchronized by serum withdrawal and subsequently stimulated with 0.001, 0.01, 0.1 and 1 μM estradiol (E(2)) in the presence or absence of platelet-derived growth factor BB (PDGF-BB) for 24 h. E(2) reduced mRNA-expression of hyaluronic acid synthase (HAS) 1 in the presence and absence of PDGF-BB. In contrast, HAS2- and HAS3-mRNA-expression were not affected. This E(2)-mediated effect on HAS1 mRNA-expression was accompanied by reduced hyaluronan secretion and a shift of HA toward lower molecular weight as evidenced by molecular sieve chromatography. The downregulation of HAS1 was abrogated by the estrogen receptor (ER) α and β antagonist ICI182780 and could be mimicked by the ERα-agonist propyl-pyrazole triol (PPT). On the contrary, the ERβ-agonist diarylpropionitrile (DPN) had no effect on HAS1 mRNA-expression. To investigate whether the downregulation of HAS1 was causally involved in the phenotypic regulation of human VSMC by E(2), lentiviral overexpression of HAS1 was conducted. Overexpression of HAS1 abrogated the inhibition of sustained ERK1/2 phosphorylation and in turn inhibition of DNA-synthesis by E(2). For the first time this study provides strong evidence that HAS1-driven HA-synthesis is a target of E(2) in human VSMC and that E(2) mediates part of its anti-proliferative effects through an ERα-dependent inhibition of HA-synthesis.
Similar threads
Thread starter Title Forum Replies Date
Julian Low Estradiol and Low DHEA-S (Joint Pain, Night Sweats, Paresthesia) Blood Work, Labs 24
IVILA What explains high estrone but low estradiol? Ask For Help or Advice 5
Mito The association between testosterone, estradiol and their ratio and mortality among US men Scientific Studies 1
F T-Gel on my stomach to drastically boost Estradiol when deficient was recreational/euphoric for me Thyroid and Hormones 9
F How to increase blood clotting or estradiol (prothrombin factors)? Ask For Help or Advice 9
F How to get the most E2 Estradiol out of DHEA supplementation? Hormones 12
shine Social isolation stimulates hippocampal estradiol synthesis Hormones 0
GreekDemiGod Peaty supplements / diet increased my Prolactin and Estradiol. Why? Ask For Help or Advice 25
Lokzo Elevated Serum Estradiol Levels May Impair Erectile Function Scientific Studies 2
V Low Estradiol: Serum, Saliva, Urine Experiments 11
Cecilia L. High Pregnenolone, High Progesterone, High Estradiol, Low D Female Issues 13
C Obese, Low Estradiol And High Progesterone? Blood Work, Labs 7
md_a Both, Female And Male COVID-19 Patients May Present Elevated Estradiol Levels Articles & Scientific Studies 0
I Hack My Labs: Lowish T, Very Low Estradiol, High Evening Cortisol Blood Work, Labs 15
haidut Elevated Maternal Estrogen (estradiol) Again Linked To Autism Scientific Studies 5
haidut While Estradiol Falls, Estrone Rises With Age And Is Much More Relevant For Estrogen-driven Diseases Scientific Studies 6
RealNeat Cat's Claw Binds Estradiol By 47.2% Supplements 10
A A New Randomized Control Trial Finds That Estradiol Improves The Symptoms Of Schizophrenia In Women Scientific Studies 15
B How To Balance Lower Estradiol? Ask For Help or Advice 1
K How To Properly Reduce Estradiol? Hormones 44
G Low Estradiol Male 22YO Blood Work, Labs 16
A Bloodwork Help-Total T, Free T, DHT, Estradiol All Low Male Issues 0
S Maternal Nest-building Is Regulated By The Hormonal Actions Of Estradiol, Progesterone, And Prolacti Thyroid and Hormones 0
ddjd Estradiol E2 Is 90 Pg/ml. What To Do? Male Issues 1
P Potential PSSD Treatment - Estradiol And DHT - Dosage Questions Male Issues 1
R DHT Gel Shuts Down LH, FSH, Estradiol And T But Sustained Sexual Function Ask For Help or Advice 3
Let Go Rapid Effects Of Estradiol On Aggression In Birds And Mice: The Fast And The Furious Scientific Studies 2
R Estrogen/Estradiol & Masculinity Thyroid and Hormones 3
P Estradiol Decreases Taurine Level By Reducing Cysteine Sulfinic Acid Decarboxylase Via The Estrogen Articles & Scientific Studies 2
haidut Estradiol In Birth Control Pills Causes Seizures Scientific Studies 2
P The Role Of Dopamine In Methylene Blue-mediated Inhibition Of Estradiol Benzoate-induced Anterior Pi Scientific Studies 4
haidut Estrogen (estradiol) Promotes Autoimmunity Scientific Studies 6
Ulla Hormonal combination of estradiol and norgestrel- pcos theraphy? Female Issues 29
S Estradiol And Testosterone Have Opposite Effects On Microtubule Polymerization Articles & Scientific Studies 3
haidut Estradiol / estrone strongly correlated with breast cancer risk Scientific Studies 1
sweetpeat Progesterone To Estradiol Ratio (P:E2) Labs 8
haidut Estradiol promotes sudden cardiac death; progesterone is protective Scientific Studies 0
haidut Estradiol prevents benefits of resistance exercise Scientific Studies 0
haidut Estrogen (estradiol) Is The Cause Of Varicose Veins Scientific Studies 16
F Test estrogen or estradiol? Blood Work, Labs 6
S Estradiol masculinizes the brain through Prostaglandins Articles & Scientific Studies 3
S Estrogen dominance / estradiol -- need advice Male Issues 5
S High Estradiol, low Estrone? also viruses Ask For Help or Advice 7
Stunning4keke Estradiol Transdermal Patches & Prometrium Female Issues 4
R Calcium in milk products precipitates intestinal fatty acids and secondary bile acids and thus inhibits colonic cytotoxicity in humans Diet 6
L SARS-CoV-2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro Scientific Studies 4
sibyloftherhine New Study Reveals that Lactoferrin Inhibits SARS-CoV-2 Infection Breaking News 1
C Grape Seed Extract inhibits aromatase Supplements 4
Braveheart Bromelain Inhibits SARS-CoV-2 Infection in VeroE6 Cells Viruses, Parasites, Fungus, Vaccines 0
meatbag Stop recommending Ivermectin, it inhibits Importin α/β, an esential protein for cellular processes Scientific Studies 88

Similar threads