Mito
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- Joined
- Dec 10, 2016
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Conclusions
The argument that the spike proteins synthesized by codon optimized mRNAs are identical to spike protein from the virus should be cautiously examined. There are several arguments that challenge this dogma. First, the biodistribution of non-specific LNP transfection of mRNAs does not discriminate towards ACE2 or CD147 expressing cell lines as seen with the virus. Second, the mRNA that encodes spike protein is known to be different in several regards. The mRNAs are known to have a 2 Proline substitution (K986P and V987P) (Department of Health and Human Services Patent US 10,960,070B2) altering the proteins conformation. The mRNAs are known to be codon optimized thus altering their secondary structure and their quadruplex G density in the spike protein mRNA. The mRNAs are known to have N1-methylpseudourine substitutions that alter translation fidelity and Toll Like Receptor recognition. Additionally, the expression levels and duration of these mRNAs may be longer and of higher copy number in many tissues that never experience natural virus infection. Finally, the pharmacokinetics of injection are different than infection. 60ug-200ug of Spike mRNA equates to 26 Trillion to 80 Trillion mRNA molecules injected in a few seconds. The pharmacokinetics of this bolus injection differs from that of viral replication that occurs over the course of a few days. If each of these mRNAs can produce 10-100 spike proteins and you have 30-40 Trillion cells, there may be a far greater systemic quantity and a much longer duration of spike protein exposure through the vaccination route than natural infection. Boosters given more frequently than a year will lead to total body accumulation of spike protein and further heighten the risk of disease in organs such as the brain, heart, bone marrow, and immune cells and tissues. This false equivalency may lead to an under appreciation of the symptomatology of vaccine based adverse events.
It should be emphasized that these results are an in-silico hypothesis supported by the peer- reviewed literature but further work is required to better characterize the homogeneity of spike protein expression in-vivo. This work has not considered post translational modifications or the impact of the degenerate base pairing from N1-methylpseudouridine.
More than 20 months into this pandemic and we have millions of SARs-CoV-2 genomes sequenced. Lot to lot sequencing of the vaccines is non-existent. To this date, no raw reads for these vaccines exist in NCBI despite over a billion liability-free vaccinations. To fully understand RNA synthesis substitution errors, fragmentation errors or strandedness errors in the mRNA synthesis process, robust lot to lot sequencing should be performed and published. Given these mRNAs are prodrugs which code for a desired protein, where is the evidence that the conversion of this prodrug into a drug is of high fidelity? This seems to have been assumed as opposed to documented. This work suggests this assumption should be questioned. Public and transparent quality control of these often-mandated injections are required. This should include sequence verification and quality control of the various lots and evidence of the proteins these mRNA express in patients.
The argument that the spike proteins synthesized by codon optimized mRNAs are identical to spike protein from the virus should be cautiously examined. There are several arguments that challenge this dogma. First, the biodistribution of non-specific LNP transfection of mRNAs does not discriminate towards ACE2 or CD147 expressing cell lines as seen with the virus. Second, the mRNA that encodes spike protein is known to be different in several regards. The mRNAs are known to have a 2 Proline substitution (K986P and V987P) (Department of Health and Human Services Patent US 10,960,070B2) altering the proteins conformation. The mRNAs are known to be codon optimized thus altering their secondary structure and their quadruplex G density in the spike protein mRNA. The mRNAs are known to have N1-methylpseudourine substitutions that alter translation fidelity and Toll Like Receptor recognition. Additionally, the expression levels and duration of these mRNAs may be longer and of higher copy number in many tissues that never experience natural virus infection. Finally, the pharmacokinetics of injection are different than infection. 60ug-200ug of Spike mRNA equates to 26 Trillion to 80 Trillion mRNA molecules injected in a few seconds. The pharmacokinetics of this bolus injection differs from that of viral replication that occurs over the course of a few days. If each of these mRNAs can produce 10-100 spike proteins and you have 30-40 Trillion cells, there may be a far greater systemic quantity and a much longer duration of spike protein exposure through the vaccination route than natural infection. Boosters given more frequently than a year will lead to total body accumulation of spike protein and further heighten the risk of disease in organs such as the brain, heart, bone marrow, and immune cells and tissues. This false equivalency may lead to an under appreciation of the symptomatology of vaccine based adverse events.
It should be emphasized that these results are an in-silico hypothesis supported by the peer- reviewed literature but further work is required to better characterize the homogeneity of spike protein expression in-vivo. This work has not considered post translational modifications or the impact of the degenerate base pairing from N1-methylpseudouridine.
More than 20 months into this pandemic and we have millions of SARs-CoV-2 genomes sequenced. Lot to lot sequencing of the vaccines is non-existent. To this date, no raw reads for these vaccines exist in NCBI despite over a billion liability-free vaccinations. To fully understand RNA synthesis substitution errors, fragmentation errors or strandedness errors in the mRNA synthesis process, robust lot to lot sequencing should be performed and published. Given these mRNAs are prodrugs which code for a desired protein, where is the evidence that the conversion of this prodrug into a drug is of high fidelity? This seems to have been assumed as opposed to documented. This work suggests this assumption should be questioned. Public and transparent quality control of these often-mandated injections are required. This should include sequence verification and quality control of the various lots and evidence of the proteins these mRNA express in patients.
