http://www.jappl.org/content/95/4/1584.full
A novel aspect of this study was to conduct the thyroid ablation after cold acclimation had been achieved, resulting in a hypothyroid state associated with normal body temperature and survival. This approach allowed us to assess the effects of cold-induced NST in tissues free of thyroid-induced thermogenesis. In contrast, room temperature-adapted hypothyroid rats are unsuitable for these studies, because the animals rapidly develop hypothermia and die within hours of cold exposure.