Cells From Dead Bodies Stay Alive And Functional For More Than 2 Weeks

[Orion]

Thanks! Not sure if you saw it, but lucenzo01 is also a former user Accutane and has a thread where he shared his great results.

Thanks, will take a look.

 

[Tarmander]

I was on Danny's podcast last month (January 2018) and should be on again this coming Friday. I thought he announced it on his page a few times??

I guess you have to be a patreon to get the podcast

 

[Wagner83]

I guess you have to be a patreon to get the podcast

1 dollar a month. I don't remember if one can unsuscribe easily.

 

[paymanz]

At least one of those Indian man spent 15 days in a hospital ward under observation of doctors and he did just fine with no food or water. I am not sure why this is not bigger news as if true it would be groundbreaking.
Docs Baffled by Man Who Doesn't Eat or Drink
"...Jani spent more than two weeks in April and May under the observation of doctors at Sterling Hospital in the western Indian city of Ahmedabad. "We studied him for 15 days with him taking no water or food," Dr. Sudhir Shah told ABC News. Shah said that Jani gargled water and took baths, but consumed nothing. While thin, Jani is healthy, doctors said. "Somebody doesn't take water for seven or eight days he surely dies," Shah said. Perhaps as equally interesting for the doctors was the fact that Jani passed no urine or stool during the time period. Shah said the normally when someone has no stool or urine, they need dialysis. Jani has confounded the scientists. "We are studying the phenomenon," Shah said."

They monitored him for 15 day simply because he cant go longer.

 

[haidut]

They monitored him for 15 day simply because he cant go longer.

I don't think anybody "normal" can survive without water for 15 days. Food - yes, but not water. The maximum recorded survival without water is about 6 days and for most people is 3-4 days. Even if this guy turns out to not be able to tolerate more than 2 weeks without water that's still pretty impressive and I would like to know how he does it.
The 15 days maximum was probably for medical ethics reasons. Since it is considered possibly life threatening experiment, the hospital which admitted him probably had to cap the experiment out of fear he will die in their care and they will get sued or attacked. I am pretty sure there are laws on that in India as there are in the US. The guy apparently has a huge following in India and as a hospital you don't want to be known as the place that got the best of him

 

[paymanz]

I don't think anybody "normal" can survive without water for 15 days. Food - yes, but not water. The maximum recorded survival without water is about 6 days and for most people is 3-4 days. Even if this guy turns out to not be able to tolerate more than 2 weeks without water that's still pretty impressive and I would like to know how he does it.
The 15 days maximum was probably for medical ethics reasons. Since it is considered possibly life threatening experiment, the hospital which admitted him probably had to cap the experiment out of fear he will die in their care and they will get sued or attacked. I am pretty sure there are laws on that in India as there are in the US. The guy apparently has a huge following in India and as a hospital you don't want to be known as the place that got the best of him

Yes thats impressive to not drink water for more than a week, that man certainly has adapted himself to it over years of training.

Just like that story once ray mentioned in one of his emails ,that a man adapted himself to drink sea water and stay alive.


Btw not eating food in same period probably helps to stay longer without water.

 

[paymanz]

And from what i read on his wikipedia page in oe of experiments his weight slightly decreased after fasting! So another sign that says his claim are not true.

 

[haidut]

And from what i read on his wikipedia page in oe of experiments his weight slightly decreased after fasting! So another sign that says his claim are not true.

Yeah, I don't buy the claim (yet) he can subsist on "ether" or electricity like these guys:
Biologists discover electric bacteria that eat pure electrons rather than sugar, redefining the tenacity of life - ExtremeTech

But then again, if bacteria can do it then maybe higher life forms can do it as well through some form of adaptation/training?? Maybe at least partially?? Certainly thought provoking.

 

[paymanz]

Yeah, I don't buy the claim (yet) he can subsist on "ether" or electricity like these guys:
Biologists discover electric bacteria that eat pure electrons rather than sugar, redefining the tenacity of life - ExtremeTech

But then again, if bacteria can do it then maybe higher life forms can do it as well through some form of adaptation/training?? Maybe at least partially?? Certainly thought provoking.

Interesting.

 

[paymanz]

But we are highest form of life, at least on earth , we have such a amazing brain that our system is desinated to serve it.

We have much much more limitations in shifting directions biological wise,compared to a bacteria or other primal types of life forms . I dont think thats possible.


But is the same time maybe our brain has abilities that we dont know maybe it can do magical stuff.

So maybe im wrong!

 

[paymanz]

Entombed animal - Wikipedia

 

[TreasureVibe]

Don't they say that if you die your brain cells get damaged beyond repair due to lack of oxygen?

 

[cedric]

Docs Baffled by Man Who Doesn't Eat or Drink

Melanins to the rescue?

 

[area51puy]

I mentioned on a few of Danny Roddy's shows about the current experiments with so-called "suspended animation".
Suspended animation - Wikipedia

Those experiments proved that an organism can be made completely dead (legally speaking) with no heartbeat, no brain activity and no organ function, and it can be kept in that state for days. After that, upon reintroduction of blood and resuscitation, the organism comes back to life and appears completely normal. Up until last year, the experiments had been conducted only with animals, but news leaked that a few US military sites successfully applied the technique to critically injured solders, and it worked just as well.
Aside from the thorny questions this raises about what "dead" really means, it also raises the uncomfortable possibility that "dead" people are being buried while still in a state where they can possibly be revived. In support of this "death as a process as opposed to an event" idea, the study below found that some cells from "dead" humans remain alive and viable for more than 2 weeks, and are completely functional when transplanted in a "living" body. The cells enter a state not unlike the one of suspended animation (or hibernation) and dramatically slow down their metabolism in order to conserve energy.
This reminds me of Peat's article on a toad that had been "buried" accidentally in concrete when a new house was being built and spending several decades in this state of hibernation. When the house was being demolished and the toad was found by workers, it quickly revived when they poured some water on it. A similar process of truly suspended animation is employed by the tardigrade (water bear), and it is thought that the mechanism responsible for this amazing feat of survival is the animal's usage or the sugar trehalose in its cells.
Tardigrade - Wikipedia
"...Tardigrades are one of the few groups of species that are capable of suspending their metabolism (see cryptobiosis). Many species of tardigrade can survive in a dehydrated state up to five years, or in exceptional cases longer.[42][43] Depending on the environment, they may enter this state via anhydrobiosis, cryobiosis, osmobiosis, or anoxybiosis. While in this state, their metabolism lowers to less than 0.01% of normal and their water content can drop to 1% of normal.[34] Their ability to remain desiccated for such long periods was thought to be largely dependent on the high levels of the nonreducing sugar trehalose, which protects their membranes, although recent research suggests that tardigrades have a unique type of disordered protein that serves a similar purpose: It replaces water in the cells and adopts a glassy, vitrified state when the animals dry out.[44] Their DNA is further protected from radiation by a protein called "dsup" (short for damage suppressor).[45][46] In this cryptobiotic state, the tardigrade is known as a tun.[47]"

Peat wrote about trehalose, which is a non-reducing sugar, and its ability to stabilize cells and protect them from radiation, poison and other assaults. Another non-reducing sugar is sucrose. I don't know if these sugars can perform the same feats in humans, but it certainly seems likely. It remains to be seen if trehalose/sucrose will be used in those suspended animation experiments with humans when they become more widespread.

Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity
After death, some stem cells remain alive for days | FierceBiotech

"...By becoming dormant, skeletal muscle stem cells can survive in a human body after a person dies, for a good 17 days after the fact. During that time, it turns out, they're still viable enough to be revived and then subdivide into workable cells, Fabrice Chretien of the Pasteur Institute in Paris and his colleagues have discovered. Their finding, published in the journal Nature Communications, could someday allow for patients to donate their stem cells, post-mortem, for use as a possible tissue rebuilding treatment.
Previously, these kind of stem cells were thought to survive for no more than a day or two, but the research team's data on the survival skills of human skeletal stem cells in a dead body explodes that notion. The same thing goes for mice: The researchers determined that these same kind of stem cells remain alive in rodents for about 16 days, the Daily Mail, Agence France Press, and others reported. Separately, they learned that bone marrow stem cells in mice survived for about four days after death and could still rebuild tissue in the wake of a bone marrow transplant.
The key to remaining viable: The skeletal stem cells slowed their metabolism until they became dormant, allowing them to conserve vital energy even as the body in which they are housed dies around them."

@haidut


View: https://youtu.be/0vc0TTQZEcU?si=2QuS9OWyBdGtWN67


Have you come across the studies discussing how tardigrades survive in extreme conditions by transforming their cells into a gel-like substance instead of a vitrified state? So is this why or one of the reasons glycine and gelatin are beneficial because they help maintain our cells in a gel-like state, as opposed to a state with a cell membrane that is more water-like inside. If the cell is in a gel like state, could it potentially protect the internal processes and overall function of the cell?

Tardigrades use gel for survival

Tardigrades have lived on our planet for millions of years. They excel at surviving in all kinds of extreme conditions by draining all the water from their bodies. But don't cells need water to survive? Researchers from the University of Tokyo discovered that water bears survive in this dried...

www.micropia.nl

. This protein is called cytoplasmic-abundant heat soluble (CAHS) and is found only in tardigrades. Once the cell dries out, these proteins start to form gel-like threads, which strengthen the cell and keep it in shape. When water then re-enters the cell, the threads retract.

Stress-dependent cell stiffening by tardigrade tolerance proteins that reversibly form a filamentous network and gel

Tardigrades are able to tolerate almost complete dehydration by entering a reversible state called anhydrobiosis and resuming their animation upon rehydration. A study of the stress-dependent condensing properties of tardigrade tolerance proteins reveals how they work to enhance the physical...

journals.plos.org

In the previous study, CAHS proteins were suggested to act as a vitrifying agent like trehalose during dehydration based on the shift in DSC [18], but this hypothesis was recently counter-argued with data demonstrating that the shift in DSC can be explained by water retention of CAHS proteins [21]. Because hydrogel generally has high water retention properties, our observation of gel transition by CAHS proteins supports the water retention in the counterargument rather than vitrification. In vitro gel transition was observed when using a relatively high concentration (approximately 4 mg/mL) of CAHS protein solution (Figs 3E, S22C and S22D), and the filament-impaired CAHS mutants failed in transition to gel (Fig 3F), suggesting that a dense filament formation is the structural basis for the gel transition of CAHS proteins. To confirm the protein concentration used in gel transition in vitro is physiologically relevant, we estimated the amount of endogenous CAHS3 proteins in R. varieornatus by immunoblotting analysis, indicating that the amount of CAHS3 proteins is about 3.8 ng per individual (S24 Fig; see Materials and methods). The wet weight of a single individual of R. varieornatus was reported to be 1.84 μg [36], which roughly corresponds 1.84 nL, and thus our rough estimate of the concentration of endogenous CAHS3 protein is 2 mg/mL. Considering that CAHS3 proteins are present only in the cytosol and not in the nucleus or extracellular space, the physiological concentration of CAHS3 proteins would be much higher than our estimate, and we assumed it is in a similar range of the concentration used in the gel transition experiments in vitro. Considering the cell volume reduction during dehydration that leads to a significant increase in both the protein concentration and ion strength that might be one of the gel-inducing factors as shown in S23A Fig, the intracellularly abundant CAHS proteins could undergo gel transition in tardigrade cells and provide mechanical stabilization of cell integrity during dehydration (Fig 4C). This gel transition could partly account for the exceptional stability of dehydrated tardigrades.

 

[sunny]

One caution regarding trehalose

Dietary sugar linked to increasing bacterial epidemics

"The researchers investigated what sources of food RT027 and RT078 preferred. They discovered that these lineages can grow on levels of sugar trehalose that are about 1,000 times lower than those needed by other lineages of these bacteria, giving RT027 and RT078 a major advantage. Each lineage is highly efficient at using trehalose and evolved independent mechanisms to utilize this sugar. To connect the ability to metabolize low levels of trehalose with increased disease severity, the researchers worked with a mouse model of C. difficile infection."

"Further experiments showed that increased disease severity in the presence of trehalose could not be explained by the mice having higher numbers of bacteria, instead what made the disease more severe was that RT027 produced higher levels of toxins."

"These and other experiments provide evidence that dietary trehalose has contributed to the predominance of epidemic C. difficile lineages and to their virulence. Because the genetic factors that allow these bacteria to metabolize trehalose and increase the production of toxins were present well before the outbreaks started, the researchers investigated what could have triggered the epidemics."

"In 2000, trehalose was approved as a food additive in the United States for a number of foods from sushi and vegetables to ice cream, and about three years later the reports of outbreaks with these lineages started to increase," Britton said. "Other factors may also contribute, but we think that trehalose is a key trigger.""

The link is no longer available.
I wonder if the trehalose resulting in c. Difficile infections were from food additive trehalose and not naturally occurring such as that in mushrooms.

TREHALOSE

TREHALOSE

Trehalose: Benefits, Uses, Food Sources & Dangers - SelfDecode Supplements

Trehalose may support eye & heart health, but it comes with a downside. Should you swap it out for table sugar? Find out here.

supplements.selfdecode.com

"

C. Difficile Outbreaks​

A study recently published in “Nature” suggests the use of trehalose as a food additive may have triggered the uncontrolled growth of aggressive, antibiotic-resistant superbugs [52].

Trehalose may stimulate the growth of Clostridium difficile (C. diff.), a germ that causes dangerous, hard-to-treat gut infections. In one study on mice infected with certain strains of C. diff., trehalose increased the death rate 3 times. These strains thrived on trehalose and produced much more toxins [52, 53].

The authors brought up additional facts that support the potential role of trehalose in recent C. diff. Outbreaks [52]:

• The two most virulent C. diff. Strains thrived on trehalose
• The usually consumed amounts of trehalose are enough to boost their growth
• A sharp rise in C. diff. outbreaks has occurred shortly after the food industry started using trehalose on a large scale

 

[Bozidar]

Just when I thought this forum could not get any crazier, this thread pops up.
But its great

 

[area51puy]

Tardigrade proteins can protect human cells

Tardigrades are remarkable little creatures. These tiny, eight-legged omnivores can survive in almost any environment. They can be dried out, frozen, boiled, exposed to high radiation levels, and even survive…

boingboing.net

The proteins responsible for the tardigrade's near-indestructibility have long been studied for potential application to human biology. Scientists from the University of Wyoming have published a study in the journal Protein Science, which found that these proteins can also protect human cells. The lead author of the paper explains:

"Amazingly, when we introduce these proteins into human cells, they gel and slow down metabolism, just like in tardigrades," Sanchez-Martinez says. "Furthermore, just like tardigrades, when you put human cells that have these proteins into biostasis, they become more resistant to stresses, conferring some of the tardigrades' abilities to the human cells."

 

[Oleg]

Dr. Peat also mentioned that hydrogen can be a direct source of energy. Kind of like sugar but without any enzymatic activities involved. If it’s true and if someone could figure out how to use hydrogen as a food source, that would be revolutionary. I think.