Riesensackteil
Member
- Joined
- Apr 4, 2021
- Messages
- 499
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Cell culture, limiting dilution, and isolation
Vero CCL-81 cells were used for isolation and initial passage. Vero E6, Vero CCL-81, HUH 7.0, 293T, A549, and EFKB3 cells were cultured in Dulbecco’s minimal essential medium (DMEM) supplemented with heat inactivated fetal bovine serum(5 or 10%) and antibiotic/antimyotic (GIBCO). Both NP an OP swabs were used for virus isolation. For the isolation, limiting dilution, and passage 1 of the virus, 50 μl serum free DMEM was pipetted into columns 2–12 of a 96-well tissue culture plate. One-hundred μl clinical specimens were pipetted into column 1, and then serially diluted 2-fold across the plate. Vero cells were trypsinized and resuspended in DMEM + 10% FBS + 2X Penicillin-Streptomycin + 2X antibiotic − antimycotic + 2 X amphotericin B at 2.5 × 105 cells / ml. One hundred μl of cell suspension were added directly to the clinical specimen dilutions and mixed gently by pipetting. The inoculated cultures were grown in a humidified 37°C incubator with 5% CO2 and observed for cytopathic effect (CPE) daily. Standard plaque assays were used for SARS-CoV-2 based on both SARS-CoV and MERS-CoV protocols (19, 20).
isolate
[ verb ahy-suh-leyt; noun, adjective ahy-suh-lit, -leyt ]SHOW IPA
verb (used with object), i·so·lat·ed, i·so·lat·ing.
to set or place apart; detach or separate so as to be alone.
Medicine/Medical. to keep (an infected person) from contact with noninfected persons; quarantine.
Chemistry, Bacteriology. to obtain (a substance or microorganism) in an uncombined or pure state.
You distilled the essence of the deception very well.Excellent explanation. In other words, the so called "isolates" are an abstraction of reality, the "genome sequencing" is an abstraction of that abstraction, and the PCR test is an abstraction of the genome abstraction based on the "isolate" abstraction.
Gary Null made a documentary challenging a lot of the Myths of AIDS, and HIV. Many of the researchers in the documentary bring up some of the same points that Kaufman is bringing up. The problems with the "discovery" of HIV and it's "causal role" in AIDS is discussed starting around 16 minutes, and the problems with AIDS/HIV testing is discussed starting at 32 minutes (and look at some of the personalities who would later have big roles in COVID)
View: https://www.youtube.com/watch?v=FRCyk0zdBxw
Same Fraud, Different "Pandemic."
Right?I am always surprised that when the experts (in this case Bob Gallo and Anthony Fauci), say that the virus has been studied and it causes AIDS why no reporter actually challenges them on the statement and asks to review their data or studies to back up their statements (at least on camera) perhaps they do but they are dismissed or fired.
Here is Andrew Kaufman detailing the even further lunacy of the "variant discoveries," which don't even have a bogus isolation to claim that they exist, or a bogus test to detect them-
View: https://www.bitchute.com/video/3afWsXJUybnh/
This experiment got me thinking...... how did they manage to isolate and purify the "Spike Protein" from SARS-Cov-2, when they never even managed to isolate and purify this "Novel Corona Virus" from the other cellular material when they allegedly "discovered" it in the first place?
The short answer is..... they didn't. The study itself doesn't even make such a claim (contrary to how the title is written). The whole story is told in right here, in the Abstract-
Did you gloss over that word "recombinant?" You shouldn't. It means this is a synthetic protein.
What are Recombinant Proteins?
Recombinant proteins are formed by transfecting foreign genes into a host cell. They are commonly used in the pharmaceutical industry.www.news-medical.net
Recombinant DNA - Wikipedia
en.wikipedia.org
Definition of RECOMBINANT
relating to or exhibiting genetic recombination; relating to or containing genetically engineered DNA; produced by genetic engineering… See the full definitionwww.merriam-webster.com
As always, the "Materials and Methods" section gives further detail-
Here is the listing from ProSci-
SARS-CoV-2 (COVID-19) S1 Recombinant Protein
SARS-CoV-2 (COVID-19) S1 Recombinant protein - Cat. No. 97-092 | ProSciwww.prosci-inc.com
They can claim this synthetic protein is the same as the "Spike Protein" from "SARS-Cov-2, but since neither the alleged virus, nor the alleged spike protein has been isolated and purified, how would anyone possibly know?
Although, since ProSci charges over $500 for 0.1 mg (!!!!), I'm guessing they don't care. And probably prefer no one ask that question.