Anabolic ,DHT Supporting Effect Of Doxycycline In Osteoblasts

Discussion in 'Articles & Scientific Studies' started by paymanz, Feb 16, 2018.

  1. paymanz

    paymanz Member

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    Lol sorry , couldn't find better tittle for this.

    Probably low, nonantibacterial dose of doxycycline is what we need for these effects. Like 20-40mg a day.

    Antioxidant Response of Osteoblasts to Doxycycline in an Inflammatory Model Induced by C-reactive Protein and Interleukin-6


    Objectives: Investigation of osteoblastic responses to oxidative stress, induced by C-reactive protein (CRP) and IL-6 and ameliorating effects of doxycycline (Dox); using assays for 5-alpha dihydrotestosterone (DHT) as an antioxidant marker of healing. IL-6 and CRP are risk markers of periodontitis and prevalent comorbidities in periodontitis subjects. Methods: Confluent monolayer cultures of osteoblasts were incubated with radiolabelled testosterone (14C-T) as substrate, in the presence or absence (Control) of pre-determined optimal concentrations of CRP, IL-6, Dox; alone and in combination (n=8) for 24h in MEM. The eluent was solvent-extracted for steroid metabolites. They were separated using TLC in a benzene/ acetone solvent system 4:1 v/v; and quantified using radioisotope scanning. The identity of formed metabolites was confirmed using the mobility of cold standards added to the samples and disclosed in iodine. Further confirmation of the authenticity of DHT was carried out by combined gas chromatrography-mass spectrometry, after derivatization to pentafluorobenzyloxime trimethyl silyl ether. Results: The yields of DHT from 14C-testosterone showed 2-fold and 1.8-fold- inhibition in response to IL-6 and CRP respectively and 28% stimulation in response to Dox, via the 5-alpha reductase pathway. The combination of IL-6 + CRP showed a 2-fold reduction in the yields of DHT, elevated to control values when combined with Dox (n=8; p<0.001). Yields of 4-androstenedione showed an inverse relationship to those of DHT, in response to the agents tested, in keeping with the 17-beta hydroxysteroid dehydrogenase pathway. Conclusions: Inhibition of DHT synthesis in osteoblasts by IL-6 and CRP was overcome by doxycycline. Oxidative actions of IL-6 and CRP; and antioxidant actions of Dox are reinforced by the metabolic yields of DHT in response to agents tested. Using a novel metabolically active model allows closer extrapolation to in vivo conditions; in the context of adjunctive therapeutic applications for periodontitis and prevalent comorbidities.

     
  2. Vinero

    Vinero Member

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    One way of thinking about this is that doxycycline normalizes DHT production in people where DHT is suppressed due to high inflammation.
    Aspirin is another thing which lowers CRP. CRP is elevated due to infections in the body.
     
  3. Alex Jaramillo

    Alex Jaramillo Member

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    Have either of you tried doxy?
     
  4. methylenewhite

    methylenewhite Member

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    My personal anecdotal experience says doxy is anabolic.
     
  5. Alex Jaramillo

    Alex Jaramillo Member

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    Just while using or do you still feel residual effects
     
  6. methylenewhite

    methylenewhite Member

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    @Alex Jaramillo

    I did 2 weeks x 200mg per day. Lost some fat and gained some muscles. I was 3 months ago and I still feel difference but I would say this post-treatment anabolic affect is a result from improved metabolic activity rather then direct anabolic affect of doxy. Any way my grow rate is much much much better then before doxy, but Im doing lots of thing, I mean supps and regimes, and I could not attribute this improvment to doxy only.
     
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